Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | paaR-paaA-parE/- |
| Location | 2542718..2543189 | Replicon | chromosome |
| Accession | NZ_CP089270 | ||
| Organism | Escherichia coli O157:H7 strain M1300706002 | ||
Toxin (Protein)
| Gene name | parE_1 | Uniprot ID | A0A0D7C2L1 |
| Locus tag | LSI72_RS12765 | Protein ID | WP_001303511.1 |
| Coordinates | 2542718..2542996 (-) | Length | 93 a.a. |
Antitoxin (Protein)
| Gene name | paaA | Uniprot ID | Q8XAD5 |
| Locus tag | LSI72_RS12770 | Protein ID | WP_001302048.1 |
| Coordinates | 2542998..2543189 (-) | Length | 64 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LSI72_RS12735 (2537943) | 2537943..2538194 | - | 252 | WP_000005552.1 | excisionase family protein | - |
| LSI72_RS12740 (2538267) | 2538267..2540738 | - | 2472 | WP_000048458.1 | exonuclease | - |
| LSI72_RS12745 (2540831) | 2540831..2541022 | - | 192 | WP_001090200.1 | DUF1482 family protein | - |
| LSI72_RS12750 (2541019) | 2541019..2541207 | - | 189 | WP_000449192.1 | cell division inhibition protein DicB | - |
| LSI72_RS12755 (2541776) | 2541776..2541994 | - | 219 | WP_001171930.1 | protein YdfC | - |
| LSI72_RS12760 (2542066) | 2542066..2542365 | - | 300 | WP_001240334.1 | hypothetical protein | - |
| LSI72_RS12765 (2542718) | 2542718..2542996 | - | 279 | WP_001303511.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| LSI72_RS12770 (2542998) | 2542998..2543189 | - | 192 | WP_001302048.1 | hypothetical protein | Antitoxin |
| LSI72_RS12775 (2543210) | 2543210..2543581 | - | 372 | WP_001169686.1 | hypothetical protein | - |
| LSI72_RS12780 (2543679) | 2543679..2543981 | + | 303 | WP_000172738.1 | transcriptional regulator | - |
| LSI72_RS12785 (2543978) | 2543978..2544403 | + | 426 | WP_000693943.1 | toxin YdaT family protein | - |
| LSI72_RS12790 (2544426) | 2544426..2545388 | + | 963 | WP_000095669.1 | helix-turn-helix domain-containing protein | - |
| LSI72_RS12795 (2545395) | 2545395..2546135 | + | 741 | WP_000788938.1 | ATP-binding protein | - |
| LSI72_RS12800 (2546161) | 2546161..2546930 | + | 770 | Protein_2509 | DUF1627 domain-containing protein | - |
| LSI72_RS12805 (2546946) | 2546946..2547341 | + | 396 | WP_001118159.1 | DUF977 family protein | - |
| LSI72_RS12810 (2547398) | 2547398..2547982 | + | 585 | WP_000206793.1 | DUF551 domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| inside | Prophage | - | nleG7' | 2527838..2635206 | 107368 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 93 a.a. Molecular weight: 10661.25 Da Isoelectric Point: 5.5647
>T227926 WP_001303511.1 NZ_CP089270:c2542996-2542718 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP
Download Length: 279 bp
>T227926 NZ_CP123237:713603-713899 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA
Antitoxin
Download Length: 64 a.a. Molecular weight: 7463.47 Da Isoelectric Point: 8.6828
>AT227926 WP_001302048.1 NZ_CP089270:c2543189-2542998 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ
Download Length: 192 bp
>AT227926 NZ_CP123237:713901-714296 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|