227476

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2566526..2566997 Replicon chromosome
Accession NZ_CP089032
Organism Escherichia coli O157:H7 strain 6535WT

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag LSI69_RS12895 Protein ID WP_001303511.1
Coordinates 2566526..2566804 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag LSI69_RS12900 Protein ID WP_001302048.1
Coordinates 2566806..2566997 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LSI69_RS12865 (2561751) 2561751..2562002 - 252 WP_000005552.1 excisionase family protein -
LSI69_RS12870 (2562075) 2562075..2564546 - 2472 WP_000048458.1 exonuclease -
LSI69_RS12875 (2564639) 2564639..2564830 - 192 WP_001090200.1 DUF1482 family protein -
LSI69_RS12880 (2564827) 2564827..2565015 - 189 WP_000449192.1 cell division inhibition protein DicB -
LSI69_RS12885 (2565584) 2565584..2565802 - 219 WP_001171930.1 protein YdfC -
LSI69_RS12890 (2565874) 2565874..2566173 - 300 WP_001240334.1 hypothetical protein -
LSI69_RS12895 (2566526) 2566526..2566804 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LSI69_RS12900 (2566806) 2566806..2566997 - 192 WP_001302048.1 hypothetical protein Antitoxin
LSI69_RS12905 (2567018) 2567018..2567389 - 372 WP_001169686.1 hypothetical protein -
LSI69_RS12910 (2567487) 2567487..2567789 + 303 WP_000172738.1 transcriptional regulator -
LSI69_RS12915 (2567786) 2567786..2568211 + 426 WP_000693943.1 toxin YdaT family protein -
LSI69_RS12920 (2568234) 2568234..2569196 + 963 WP_000095669.1 helix-turn-helix domain-containing protein -
LSI69_RS12925 (2569203) 2569203..2569943 + 741 WP_000788938.1 ATP-binding protein -
LSI69_RS12930 (2569969) 2569969..2570738 + 770 Protein_2541 DUF1627 domain-containing protein -
LSI69_RS12935 (2570754) 2570754..2571149 + 396 WP_001118159.1 DUF977 family protein -
LSI69_RS12940 (2571206) 2571206..2571790 + 585 WP_000206793.1 DUF551 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - nleG7' 2558239..2659014 100775


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T227476 WP_001303511.1 NZ_CP089032:c2566804-2566526 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T227476 NZ_CP122880:4012306-4012584 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT227476 WP_001302048.1 NZ_CP089032:c2566997-2566806 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT227476 NZ_CP122880:4012043-4012303 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References