121309

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2618418..2618889 Replicon chromosome
Accession NZ_CP038309
Organism Escherichia coli O157:H7 strain Show KS 470-1

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag E4U84_RS13300 Protein ID WP_001303511.1
Coordinates 2618418..2618696 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag E4U84_RS13305 Protein ID WP_001302048.1
Coordinates 2618698..2618889 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4U84_RS13265 2613613..2613864 - 252 WP_000005552.1 excisionase family protein -
E4U84_RS13270 2613937..2616438 - 2502 WP_171878210.1 3'-5' exoribonuclease -
E4U84_RS13275 2616531..2616722 - 192 WP_001090200.1 DUF1482 family protein -
E4U84_RS13280 2616719..2616907 - 189 WP_000449192.1 cell division inhibition protein DicB -
E4U84_RS13285 2617308..2617472 + 165 WP_001331716.1 hypothetical protein -
E4U84_RS13290 2617476..2617694 - 219 WP_001171930.1 hypothetical protein -
E4U84_RS13295 2617766..2618065 - 300 WP_001240334.1 hypothetical protein -
E4U84_RS13300 2618418..2618696 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
E4U84_RS13305 2618698..2618889 - 192 WP_001302048.1 hypothetical protein Antitoxin
E4U84_RS13310 2618910..2619281 - 372 WP_001169686.1 hypothetical protein -
E4U84_RS13315 2619379..2619681 + 303 WP_000172738.1 transcriptional regulator -
E4U84_RS13320 2619678..2620103 + 426 WP_000693943.1 Rha family transcriptional regulator -
E4U84_RS13325 2620126..2621088 + 963 WP_000095670.1 helix-turn-helix domain-containing protein -
E4U84_RS13330 2621095..2621835 + 741 WP_000788936.1 ATP-binding protein -
E4U84_RS13335 2621861..2622630 + 770 Protein_2605 DUF1627 domain-containing protein -
E4U84_RS13340 2622646..2623041 + 396 WP_001118159.1 DUF977 family protein -
E4U84_RS13345 2623098..2623682 + 585 WP_000206793.1 DUF551 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - nleG7' 2611227..2709194 97967


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T121309 WP_001303511.1 NZ_CP038309:c2618696-2618418 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T121309 NZ_CP038309:c2618696-2618418 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT121309 WP_001302048.1 NZ_CP038309:c2618889-2618698 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT121309 NZ_CP038309:c2618889-2618698 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References