Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2569434..2569905 Replicon chromosome
Accession NZ_CP039834
Organism Escherichia coli O157:H7 strain MB41-1

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag FCV42_RS12915 Protein ID WP_001303511.1
Coordinates 2569434..2569712 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag FCV42_RS12920 Protein ID WP_001302048.1
Coordinates 2569714..2569905 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FCV42_RS12875 2564440..2564624 - 185 Protein_2517 DUF3596 domain-containing protein -
FCV42_RS12880 2564659..2564910 - 252 WP_000005552.1 excisionase family protein -
FCV42_RS12885 2564983..2567454 - 2472 WP_000048458.1 exonuclease -
FCV42_RS12890 2567547..2567738 - 192 WP_001090200.1 DUF1482 family protein -
FCV42_RS12895 2567735..2567923 - 189 WP_000449192.1 cell division inhibition protein DicB -
FCV42_RS12900 2568324..2568488 + 165 WP_001331716.1 hypothetical protein -
FCV42_RS12905 2568492..2568710 - 219 WP_001171930.1 hypothetical protein -
FCV42_RS12910 2568782..2569081 - 300 WP_001240334.1 hypothetical protein -
FCV42_RS12915 2569434..2569712 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FCV42_RS12920 2569714..2569905 - 192 WP_001302048.1 hypothetical protein Antitoxin
FCV42_RS12925 2569926..2570297 - 372 WP_001169686.1 hypothetical protein -
FCV42_RS12930 2570395..2570697 + 303 WP_000172738.1 transcriptional regulator -
FCV42_RS12935 2570694..2571118 + 425 Protein_2529 Rha family transcriptional regulator -
FCV42_RS12940 2571141..2572103 + 963 WP_000095669.1 helix-turn-helix domain-containing protein -
FCV42_RS12945 2572110..2572850 + 741 WP_000788938.1 ATP-binding protein -
FCV42_RS12950 2572876..2573645 + 770 Protein_2532 DUF1627 domain-containing protein -
FCV42_RS12955 2573661..2574056 + 396 WP_001118159.1 DUF977 family protein -
FCV42_RS12960 2574113..2574697 + 585 WP_000206793.1 DUF551 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - nleG7' 2562273..2659381 97108


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T124321 WP_001303511.1 NZ_CP039834:c2569712-2569434 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T124321 NZ_CP039834:c2569712-2569434 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT124321 WP_001302048.1 NZ_CP039834:c2569905-2569714 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT124321 NZ_CP039834:c2569905-2569714 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References