Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2511149..2511620 Replicon chromosome
Accession NZ_CP038372
Organism Escherichia coli O157:H7 strain F6294

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag E3161_RS12515 Protein ID WP_001303511.1
Coordinates 2511149..2511427 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag E3161_RS12520 Protein ID WP_001302048.1
Coordinates 2511429..2511620 (-) Length 64 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3161_RS12480 2506374..2506625 - 252 WP_000005552.1 excisionase family protein -
E3161_RS12485 2506698..2509169 - 2472 WP_000048458.1 exonuclease -
E3161_RS12490 2509262..2509453 - 192 WP_001090200.1 DUF1482 family protein -
E3161_RS12495 2509450..2509638 - 189 WP_000449192.1 cell division inhibition protein DicB -
E3161_RS12500 2510039..2510203 + 165 WP_001331716.1 hypothetical protein -
E3161_RS12505 2510207..2510425 - 219 WP_001171930.1 hypothetical protein -
E3161_RS12510 2510497..2510796 - 300 WP_001240334.1 hypothetical protein -
E3161_RS12515 2511149..2511427 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
E3161_RS12520 2511429..2511620 - 192 WP_001302048.1 hypothetical protein Antitoxin
E3161_RS12525 2511641..2512012 - 372 WP_001169686.1 hypothetical protein -
E3161_RS12530 2512110..2512412 + 303 WP_000172738.1 transcriptional regulator -
E3161_RS12535 2512409..2512834 + 426 WP_000693943.1 Rha family transcriptional regulator -
E3161_RS12540 2512857..2513819 + 963 WP_000095669.1 helix-turn-helix domain-containing protein -
E3161_RS12545 2513826..2514566 + 741 WP_000788938.1 ATP-binding protein -
E3161_RS12550 2514592..2515361 + 770 Protein_2454 DUF1627 domain-containing protein -
E3161_RS12555 2515377..2515772 + 396 WP_001118159.1 DUF977 family protein -
E3161_RS12560 2515829..2516413 + 585 WP_000206793.1 DUF551 domain-containing protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - nleG7' 2502862..2603504 100642


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(4-91)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T122097 WP_001303511.1 NZ_CP038372:c2511427-2511149 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T122097 NZ_CP038372:c2511427-2511149 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT122097 WP_001302048.1 NZ_CP038372:c2511620-2511429 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT122097 NZ_CP038372:c2511620-2511429 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE


Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References