102723

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2466061..2466532 Replicon chromosome
Accession NZ_CP028685
Organism Escherichia coli strain 111

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag C9E40_RS12480 Protein ID WP_001303511.1
Coordinates 2466061..2466339 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag C9E40_RS12485 Protein ID WP_001302048.1
Coordinates 2466341..2466532 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C9E40_RS12445 2461286..2461537 - 252 WP_000005552.1 excisionase family protein -
C9E40_RS12450 2461610..2464081 - 2472 WP_000048458.1 exonuclease -
C9E40_RS12455 2464174..2464365 - 192 WP_001090200.1 DUF1482 family protein -
C9E40_RS12460 2464362..2464550 - 189 WP_000449192.1 cell division inhibition protein DicB -
C9E40_RS12470 2465119..2465337 - 219 WP_001171930.1 hypothetical protein -
C9E40_RS12475 2465409..2465708 - 300 WP_001240334.1 hypothetical protein -
C9E40_RS12480 2466061..2466339 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C9E40_RS12485 2466341..2466532 - 192 WP_001302048.1 hypothetical protein Antitoxin
C9E40_RS12490 2466553..2466924 - 372 WP_001169686.1 hypothetical protein -
C9E40_RS12495 2467022..2467324 + 303 WP_000172738.1 transcriptional regulator -
C9E40_RS12500 2467321..2467746 + 426 WP_000693943.1 Rha family transcriptional regulator -
C9E40_RS12505 2467769..2468731 + 963 WP_000095669.1 helix-turn-helix domain-containing protein -
C9E40_RS12510 2468738..2469478 + 741 WP_000788938.1 ATP-binding protein -
C9E40_RS12515 2469504..2470273 + 770 Protein_2401 DUF1627 domain-containing protein -
C9E40_RS12520 2470289..2470684 + 396 WP_001118159.1 DUF977 family protein -
C9E40_RS12525 2470741..2471325 + 585 WP_000206793.1 DUF551 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - nleA/espI / nleH2 / nleF 2451181..2559552 108371


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T102723 WP_001303511.1 NZ_CP028685:c2466339-2466061 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T102723 NZ_CP028685:c2466339-2466061 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT102723 WP_001302048.1 NZ_CP028685:c2466532-2466341 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT102723 NZ_CP028685:c2466532-2466341 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References