115818

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2098457..2098928 Replicon chromosome
Accession NZ_CP034384
Organism Escherichia coli O157:H7 strain FRIK804

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag EJB16_RS10420 Protein ID WP_001303511.1
Coordinates 2098457..2098735 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag EJB16_RS10425 Protein ID WP_001302048.1
Coordinates 2098737..2098928 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EJB16_RS10385 2093682..2093933 - 252 WP_000005552.1 excisionase family protein -
EJB16_RS10390 2094006..2096477 - 2472 WP_000048458.1 exonuclease -
EJB16_RS10395 2096570..2096761 - 192 WP_001090200.1 DUF1482 family protein -
EJB16_RS10400 2096758..2096946 - 189 WP_000449192.1 cell division inhibition protein DicB -
EJB16_RS10410 2097515..2097733 - 219 WP_001171930.1 hypothetical protein -
EJB16_RS10415 2097805..2098104 - 300 WP_001240334.1 hypothetical protein -
EJB16_RS10420 2098457..2098735 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EJB16_RS10425 2098737..2098928 - 192 WP_001302048.1 hypothetical protein Antitoxin
EJB16_RS10430 2098949..2099320 - 372 WP_001169686.1 hypothetical protein -
EJB16_RS10435 2099418..2099720 + 303 WP_000172738.1 transcriptional regulator -
EJB16_RS10440 2099717..2100142 + 426 WP_000693943.1 Rha family transcriptional regulator -
EJB16_RS10445 2100165..2101127 + 963 WP_000095669.1 helix-turn-helix domain-containing protein -
EJB16_RS10450 2101134..2101874 + 741 WP_000788938.1 ATP-binding protein -
EJB16_RS10455 2101900..2102669 + 770 Protein_2049 DUF1627 domain-containing protein -
EJB16_RS10460 2102685..2103080 + 396 WP_001118159.1 DUF977 family protein -
EJB16_RS10465 2103137..2103721 + 585 WP_000206793.1 DUF551 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - nleG7' 2091296..2189034 97738


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T115818 WP_001303511.1 NZ_CP034384:c2098735-2098457 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T115818 NZ_CP034384:c2098735-2098457 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT115818 WP_001302048.1 NZ_CP034384:c2098928-2098737 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT115818 NZ_CP034384:c2098928-2098737 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References