121088

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 3178252..3178723 Replicon chromosome
Accession NZ_CP038290
Organism Escherichia coli O157:H7 strain TX 265-1

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag E4U08_RS16410 Protein ID WP_001303511.1
Coordinates 3178445..3178723 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag E4U08_RS16405 Protein ID WP_001302048.1
Coordinates 3178252..3178443 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4U08_RS16365 3173459..3174043 - 585 WP_000206793.1 DUF551 domain-containing protein -
E4U08_RS16370 3174100..3174495 - 396 WP_001118159.1 DUF977 family protein -
E4U08_RS16375 3174511..3175280 - 770 Protein_3210 DUF1627 domain-containing protein -
E4U08_RS16380 3175306..3176046 - 741 WP_000788936.1 ATP-binding protein -
E4U08_RS16385 3176053..3177015 - 963 WP_000095670.1 helix-turn-helix domain-containing protein -
E4U08_RS16390 3177038..3177463 - 426 WP_000693943.1 Rha family transcriptional regulator -
E4U08_RS16395 3177460..3177762 - 303 WP_000172738.1 transcriptional regulator -
E4U08_RS16400 3177860..3178231 + 372 WP_001169686.1 hypothetical protein -
E4U08_RS16405 3178252..3178443 + 192 WP_001302048.1 hypothetical protein Antitoxin
E4U08_RS16410 3178445..3178723 + 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
E4U08_RS16415 3179076..3179375 + 300 WP_001240334.1 hypothetical protein -
E4U08_RS16420 3179447..3179665 + 219 WP_001171930.1 hypothetical protein -
E4U08_RS16425 3179669..3179833 - 165 WP_001331716.1 hypothetical protein -
E4U08_RS16430 3180234..3180422 + 189 WP_000449192.1 cell division inhibition protein DicB -
E4U08_RS16435 3180419..3180610 + 192 WP_001090200.1 DUF1482 family protein -
E4U08_RS16440 3180703..3183174 + 2472 WP_000048458.1 exonuclease -
E4U08_RS16445 3183247..3183498 + 252 WP_000005552.1 excisionase family protein -
E4U08_RS16450 3183533..3183720 + 188 Protein_3225 DUF3596 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - nleG7' 3087896..3187010 99114


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T121088 WP_001303511.1 NZ_CP038290:3178445-3178723 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T121088 NZ_CP038290:3178445-3178723 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT121088 WP_001302048.1 NZ_CP038290:3178252-3178443 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT121088 NZ_CP038290:3178252-3178443 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References