Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2469954..2470425 Replicon chromosome
Accession NZ_CP038405
Organism Escherichia coli O157:H7 strain ATCC 35150

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag E3153_RS12235 Protein ID WP_001303511.1
Coordinates 2469954..2470232 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag E3153_RS12240 Protein ID WP_001302048.1
Coordinates 2470234..2470425 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3153_RS12200 2465179..2465430 - 252 WP_000005552.1 excisionase family protein -
E3153_RS12205 2465503..2467974 - 2472 WP_000048458.1 exonuclease -
E3153_RS12210 2468067..2468258 - 192 WP_001090200.1 DUF1482 family protein -
E3153_RS12215 2468255..2468443 - 189 WP_000449192.1 cell division inhibition protein DicB -
E3153_RS12220 2468844..2469008 + 165 WP_001331716.1 hypothetical protein -
E3153_RS12225 2469012..2469230 - 219 WP_001171930.1 hypothetical protein -
E3153_RS12230 2469302..2469601 - 300 WP_001240334.1 hypothetical protein -
E3153_RS12235 2469954..2470232 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
E3153_RS12240 2470234..2470425 - 192 WP_001302048.1 hypothetical protein Antitoxin
E3153_RS12245 2470446..2470817 - 372 WP_001169686.1 hypothetical protein -
E3153_RS12250 2470915..2471217 + 303 WP_000172738.1 transcriptional regulator -
E3153_RS12255 2471214..2471639 + 426 WP_000693943.1 Rha family transcriptional regulator -
E3153_RS12260 2471662..2472624 + 963 WP_000095669.1 helix-turn-helix domain-containing protein -
E3153_RS12265 2472631..2473371 + 741 WP_000788938.1 ATP-binding protein -
E3153_RS12270 2473397..2474166 + 770 Protein_2401 DUF1627 domain-containing protein -
E3153_RS12275 2474182..2474577 + 396 WP_001118159.1 DUF977 family protein -
E3153_RS12280 2474634..2475218 + 585 WP_000206793.1 DUF551 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - nleG7' 2462793..2559222 96429


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T122443 WP_001303511.1 NZ_CP038405:c2470232-2469954 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T122443 NZ_CP038405:c2470232-2469954 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT122443 WP_001302048.1 NZ_CP038405:c2470425-2470234 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT122443 NZ_CP038405:c2470425-2470234 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References