143196

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2561320..2561791 Replicon chromosome
Accession NZ_CP045975
Organism Escherichia coli strain AUSMDU00002545

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag GJD97_RS12885 Protein ID WP_001303511.1
Coordinates 2561320..2561598 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag GJD97_RS12890 Protein ID WP_001302048.1
Coordinates 2561600..2561791 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJD97_RS12845 2556327..2556511 - 185 Protein_2511 DUF3596 domain-containing protein -
GJD97_RS12850 2556546..2556797 - 252 WP_000005552.1 excisionase family protein -
GJD97_RS12855 2556870..2559341 - 2472 WP_000048458.1 exonuclease -
GJD97_RS12860 2559434..2559625 - 192 WP_001090200.1 DUF1482 family protein -
GJD97_RS12865 2559622..2559810 - 189 WP_000449192.1 cell division inhibition protein DicB -
GJD97_RS12875 2560378..2560596 - 219 WP_001171930.1 hypothetical protein -
GJD97_RS12880 2560668..2560967 - 300 WP_001240334.1 hypothetical protein -
GJD97_RS12885 2561320..2561598 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GJD97_RS12890 2561600..2561791 - 192 WP_001302048.1 hypothetical protein Antitoxin
GJD97_RS12895 2561812..2562183 - 372 WP_001169686.1 hypothetical protein -
GJD97_RS12900 2562350..2563563 + 1214 WP_162829202.1 IS3 family transposase -
GJD97_RS12905 2563612..2563896 + 285 WP_072617017.1 transcriptional regulator -
GJD97_RS12910 2563893..2564318 + 426 WP_000693943.1 Rha family transcriptional regulator -
GJD97_RS12915 2564341..2565303 + 963 WP_000095669.1 helix-turn-helix domain-containing protein -
GJD97_RS12920 2565310..2566050 + 741 WP_000788938.1 ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - nleG7' 2556356..2659009 102653


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T143196 WP_001303511.1 NZ_CP045975:c2561598-2561320 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T143196 NZ_CP060969:688114-688428 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT143196 WP_001302048.1 NZ_CP045975:c2561791-2561600 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT143196 NZ_CP060969:688425-688841 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References