Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2467092..2467563 Replicon chromosome
Accession NZ_CP038313
Organism Escherichia coli O157:H7 strain OK1

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag E3173_RS12185 Protein ID WP_001303511.1
Coordinates 2467092..2467370 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag E3173_RS12190 Protein ID WP_001302048.1
Coordinates 2467372..2467563 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3173_RS12150 2462317..2462568 - 252 WP_000005552.1 excisionase family protein -
E3173_RS12155 2462641..2465112 - 2472 WP_000048458.1 exonuclease -
E3173_RS12160 2465205..2465396 - 192 WP_001090200.1 DUF1482 family protein -
E3173_RS12165 2465393..2465581 - 189 WP_000449192.1 cell division inhibition protein DicB -
E3173_RS12170 2465982..2466146 + 165 WP_001331716.1 hypothetical protein -
E3173_RS12175 2466150..2466368 - 219 WP_001171930.1 hypothetical protein -
E3173_RS12180 2466440..2466739 - 300 WP_001240334.1 hypothetical protein -
E3173_RS12185 2467092..2467370 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
E3173_RS12190 2467372..2467563 - 192 WP_001302048.1 hypothetical protein Antitoxin
E3173_RS12195 2467584..2467955 - 372 WP_001169686.1 hypothetical protein -
E3173_RS12200 2468053..2468355 + 303 WP_000172738.1 transcriptional regulator -
E3173_RS12205 2468352..2468777 + 426 WP_000693943.1 Rha family transcriptional regulator -
E3173_RS12210 2468800..2469762 + 963 WP_000095669.1 helix-turn-helix domain-containing protein -
E3173_RS12215 2469769..2470509 + 741 WP_000788938.1 ATP-binding protein -
E3173_RS12220 2470535..2471304 + 770 Protein_2392 DUF1627 domain-containing protein -
E3173_RS12225 2471320..2471715 + 396 WP_001118159.1 DUF977 family protein -
E3173_RS12230 2471772..2472356 + 585 WP_000206793.1 DUF551 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - nleA/espI / nleH2 / nleF 2452212..2559879 107667


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T121352 WP_001303511.1 NZ_CP038313:c2467370-2467092 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T121352 NZ_CP038313:c2467370-2467092 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT121352 WP_001302048.1 NZ_CP038313:c2467563-2467372 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT121352 NZ_CP038313:c2467563-2467372 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References