Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/RHH-RelE |
| Location | 2443..3011 | Replicon | plasmid pAMI7 |
| Accession | NC_014832 | ||
| Organism | Paracoccus aminophilus | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | E7BLD5 |
| Locus tag | G7C87_RS00025 | Protein ID | WP_013496751.1 |
| Coordinates | 2712..3011 (+) | Length | 100 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | - |
| Locus tag | G7C87_RS00020 | Protein ID | WP_168550534.1 |
| Coordinates | 2452..2715 (+) | Length | 88 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| G7C87_RS00005 (pAMI7_p01) | 258..1325 | + | 1068 | WP_228443693.1 | replication initiator protein A | - |
| G7C87_RS00010 (pAMI7_p02) | 1328..1621 | - | 294 | WP_013496748.1 | hypothetical protein | - |
| G7C87_RS00015 (pAMI7_p03) | 1621..2283 | - | 663 | WP_013496749.1 | ParA family protein | - |
| G7C87_RS00020 (pAMI7_p04) | 2452..2715 | + | 264 | WP_168550534.1 | ribbon-helix-helix protein, CopG family | Antitoxin |
| G7C87_RS00025 (pAMI7_p05) | 2712..3011 | + | 300 | WP_013496751.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| G7C87_RS00030 (pAMI7_p07) | 3737..4213 | - | 477 | WP_013496753.1 | hypothetical protein | - |
| G7C87_RS00035 (pAMI7_p08) | 4216..4977 | - | 762 | WP_013496754.1 | hypothetical protein | - |
| G7C87_RS00040 (pAMI7_p09) | 5340..7442 | + | 2103 | WP_013496755.1 | AAA family ATPase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | - | - | 1..20542 | 20542 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 100 a.a. Molecular weight: 11247.96 Da Isoelectric Point: 4.9050
>T6134 WP_013496751.1 NC_014832:2712-3011 [Paracoccus aminophilus JCM 7686]
MKQLVIAEPAGRDLEEIVDYIAVNNPVAAEKVYWEIVRAAEKLSDFPALGRPGRHPETRELSIADLPYLLVYEVRPEEVT
ILAVFHTSRDLAKVLHDRI
MKQLVIAEPAGRDLEEIVDYIAVNNPVAAEKVYWEIVRAAEKLSDFPALGRPGRHPETRELSIADLPYLLVYEVRPEEVT
ILAVFHTSRDLAKVLHDRI
Download Length: 300 bp
>T6134 NC_014832:2712-3011 [Paracoccus aminophilus JCM 7686]
TTGAAGCAGCTGGTCATTGCCGAACCTGCAGGCCGAGATCTTGAAGAGATCGTGGATTACATCGCTGTGAATAATCCGGT
CGCCGCAGAGAAAGTATATTGGGAAATTGTGCGCGCGGCAGAGAAGTTGTCTGACTTTCCGGCACTAGGTCGCCCGGGTC
GTCATCCCGAGACACGGGAGCTCAGTATCGCGGATTTACCCTACTTGCTCGTCTATGAAGTTAGGCCCGAAGAAGTGACG
ATATTAGCAGTATTCCACACCTCCCGCGATTTGGCAAAAGTGCTGCATGACAGGATATAG
TTGAAGCAGCTGGTCATTGCCGAACCTGCAGGCCGAGATCTTGAAGAGATCGTGGATTACATCGCTGTGAATAATCCGGT
CGCCGCAGAGAAAGTATATTGGGAAATTGTGCGCGCGGCAGAGAAGTTGTCTGACTTTCCGGCACTAGGTCGCCCGGGTC
GTCATCCCGAGACACGGGAGCTCAGTATCGCGGATTTACCCTACTTGCTCGTCTATGAAGTTAGGCCCGAAGAAGTGACG
ATATTAGCAGTATTCCACACCTCCCGCGATTTGGCAAAAGTGCTGCATGACAGGATATAG
Antitoxin
Download Length: 88 a.a. Molecular weight: 9754.12 Da Isoelectric Point: 9.7317
>AT6134 WP_168550534.1 NC_014832:2452-2715 [Paracoccus aminophilus JCM 7686]
MEHTMPQRTASEPITIRTAKVAEIDALAGAMDRSRNYIVNQAIEQYLEANTWQMERIRDGIAATREGKVAPAGEVFAKIA
KKHGWSR
MEHTMPQRTASEPITIRTAKVAEIDALAGAMDRSRNYIVNQAIEQYLEANTWQMERIRDGIAATREGKVAPAGEVFAKIA
KKHGWSR
Download Length: 264 bp
>AT6134 NC_014832:2452-2715 [Paracoccus aminophilus JCM 7686]
ATGGAGCACACCATGCCGCAGCGCACCGCGTCCGAACCAATCACCATCCGAACGGCCAAGGTGGCTGAGATTGACGCTCT
TGCGGGCGCGATGGACCGTTCACGTAACTACATCGTCAATCAGGCGATTGAGCAGTACCTTGAAGCCAATACTTGGCAGA
TGGAACGGATTAGAGATGGTATCGCTGCCACCCGCGAAGGCAAGGTCGCGCCTGCGGGCGAGGTCTTTGCTAAGATCGCC
AAAAAACATGGCTGGTCACGTTGA
ATGGAGCACACCATGCCGCAGCGCACCGCGTCCGAACCAATCACCATCCGAACGGCCAAGGTGGCTGAGATTGACGCTCT
TGCGGGCGCGATGGACCGTTCACGTAACTACATCGTCAATCAGGCGATTGAGCAGTACCTTGAAGCCAATACTTGGCAGA
TGGAACGGATTAGAGATGGTATCGCTGCCACCCGCGAAGGCAAGGTCGCGCCTGCGGGCGAGGTCTTTGCTAAGATCGCC
AAAAAACATGGCTGGTCACGTTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T6097 | Sinorhizobium meliloti 1021 |
43.158 |
97.938 |
0.423 |
| T10139 | Pseudomonas savastanoi |
39.759 |
91.209 |
0.363 |
| T10119 | Escherichia coli |
40.244 |
89.13 |
0.359 |
| T10143 | Pseudomonas savastanoi |
35.227 |
95.652 |
0.337 |
| T178 | Vibrio cholerae O1 biovar El Tor str. N16961 |
32.653 |
98.99 |
0.323 |
| T659 | Shewanella oneidensis MR-1 |
34.066 |
93.814 |
0.32 |
| T1008 | Escherichia coli O157:H7 str. Sakai |
31.522 |
100 |
0.315 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT10191 | Synechococcus sp. CB0101 |
36 |
90.361 |
0.325 |
| AT180 | Pseudomonas aeruginosa PAO1 |
40.351 |
76 |
0.307 |
Multiple sequence alignment
References
(1) Lukasz Dziewit et al. (2011) Functional characterization of the type II PamI restriction-modification system derived from plasmid pAMI7 of Paracoccus aminophilus JCM 7686. FEMS Microbiology Letters 324(1):56-63. [PubMed:22092764]