Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/- |
| Location | 1668947..1669430 | Replicon | chromosome |
| Accession | NZ_CP039373 | ||
| Organism | Synechococcus sp. CB0101 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | A0A4P7ZVY3 |
| Locus tag | CB0101_RS08920 | Protein ID | WP_010312477.1 |
| Coordinates | 1669176..1669430 (+) | Length | 85 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | - |
| Locus tag | CB0101_RS08915 | Protein ID | WP_210409950.1 |
| Coordinates | 1668947..1669198 (+) | Length | 84 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| CB0101_RS08900 (CB0101_08885) | 1666257..1667003 | - | 747 | WP_010312480.1 | peptidylprolyl isomerase | - |
| CB0101_RS08910 (CB0101_08895) | 1667213..1668895 | + | 1683 | WP_043718336.1 | glycosyltransferase family 4 protein | - |
| CB0101_RS08915 (CB0101_08900) | 1668947..1669198 | + | 252 | WP_210409950.1 | ribbon-helix-helix protein, CopG family | Antitoxin |
| CB0101_RS08920 (CB0101_08905) | 1669176..1669430 | + | 255 | WP_010312477.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| CB0101_RS08925 (CB0101_08910) | 1669438..1670601 | - | 1164 | WP_071778172.1 | ATP-binding protein | - |
| CB0101_RS08930 (CB0101_08915) | 1670668..1670880 | + | 213 | WP_010312474.1 | UPF0175 family protein | - |
| CB0101_RS08935 (CB0101_08920) | 1670962..1671942 | + | 981 | WP_136644065.1 | IS5 family transposase | - |
| CB0101_RS08940 (CB0101_08925) | 1672282..1673260 | - | 979 | Protein_1808 | IS5 family transposase | - |
| CB0101_RS08945 (CB0101_08930) | 1673562..1674002 | - | 441 | WP_136644066.1 | GNAT family N-acetyltransferase | - |
| CB0101_RS08950 (CB0101_08935) | 1673999..1674364 | - | 366 | WP_043718315.1 | type II toxin-antitoxin system VapC family toxin | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 85 a.a. Molecular weight: 9533.32 Da Isoelectric Point: 10.4534
>T10191 WP_010312477.1 NZ_CP039373:1669176-1669430 [Synechococcus sp. CB0101]
MCCSLRIKRSAAKALAELPNADRLRLVAAIDKLCEVPAAGSALKGEFEGLRRLRVGRYRIVCEWQQQELVVLVVRVGHRK
EVYR
MCCSLRIKRSAAKALAELPNADRLRLVAAIDKLCEVPAAGSALKGEFEGLRRLRVGRYRIVCEWQQQELVVLVVRVGHRK
EVYR
Download Length: 255 bp
>T10191 NZ_CP039373:1669176-1669430 [Synechococcus sp. CB0101]
ATGTGCTGCTCGCTGCGGATTAAACGCAGCGCTGCCAAGGCGCTGGCTGAGCTTCCCAACGCCGACCGCCTCCGCCTCGT
GGCTGCCATCGACAAGCTTTGCGAGGTTCCGGCAGCTGGCTCAGCGCTCAAGGGCGAATTCGAGGGACTGCGTCGCCTGC
GCGTGGGTCGCTATCGGATCGTGTGCGAGTGGCAGCAGCAGGAGCTGGTGGTTCTCGTGGTGCGTGTTGGGCATCGCAAG
GAGGTGTATCGCTAA
ATGTGCTGCTCGCTGCGGATTAAACGCAGCGCTGCCAAGGCGCTGGCTGAGCTTCCCAACGCCGACCGCCTCCGCCTCGT
GGCTGCCATCGACAAGCTTTGCGAGGTTCCGGCAGCTGGCTCAGCGCTCAAGGGCGAATTCGAGGGACTGCGTCGCCTGC
GCGTGGGTCGCTATCGGATCGTGTGCGAGTGGCAGCAGCAGGAGCTGGTGGTTCTCGTGGTGCGTGTTGGGCATCGCAAG
GAGGTGTATCGCTAA
Antitoxin
Download Length: 84 a.a. Molecular weight: 9241.45 Da Isoelectric Point: 3.9011
>AT10191 WP_210409950.1 NZ_CP039373:1668947-1669198 [Synechococcus sp. CB0101]
MTLLECREALMAQVTARLPDDLTAELDAVAQQLNRCRADVIRQAIEYYLDDIEDLRAGAASLRDPADPVLDWAEVRDVLL
AAD
MTLLECREALMAQVTARLPDDLTAELDAVAQQLNRCRADVIRQAIEYYLDDIEDLRAGAASLRDPADPVLDWAEVRDVLL
AAD
Download Length: 252 bp
>AT10191 NZ_CP039373:1668947-1669198 [Synechococcus sp. CB0101]
TTGACTTTGCTTGAATGTCGGGAGGCGTTGATGGCTCAGGTGACGGCCCGACTGCCCGATGACCTGACGGCCGAACTCGA
TGCGGTCGCGCAGCAGCTGAATCGATGCCGAGCTGACGTGATTCGCCAGGCCATCGAGTACTACCTCGATGACATCGAAG
ATCTACGAGCAGGAGCCGCTTCCCTGAGGGATCCCGCCGATCCTGTTCTGGATTGGGCTGAGGTGCGCGATGTGCTGCTC
GCTGCGGATTAA
TTGACTTTGCTTGAATGTCGGGAGGCGTTGATGGCTCAGGTGACGGCCCGACTGCCCGATGACCTGACGGCCGAACTCGA
TGCGGTCGCGCAGCAGCTGAATCGATGCCGAGCTGACGTGATTCGCCAGGCCATCGAGTACTACCTCGATGACATCGAAG
ATCTACGAGCAGGAGCCGCTTCCCTGAGGGATCCCGCCGATCCTGTTCTGGATTGGGCTGAGGTGCGCGATGTGCTGCTC
GCTGCGGATTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T6285 | Streptomyces cattleya NRRL 8057 = DSM 46488 |
40 |
95.238 |
0.381 |
| T1729 | Acidithiobacillus caldus |
34.831 |
100 |
0.369 |
| T10158 | Streptococcus suis SC84 |
35.714 |
100 |
0.357 |
| T10123 | Paracoccus phage vB_PkoS_Pkon1 |
32.911 |
95.181 |
0.313 |
| T10190 | Pseudomonas sp. strain ANT_J3 |
23.009 |
100 |
0.31 |
| T173 | Vibrio cholerae O1 biovar El Tor str. N16961 |
29.885 |
100 |
0.31 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT6134 | Paracoccus aminophilus |
36 |
90.361 |
0.325 |
Multiple sequence alignment
References
(1) David Marsan et al. (2017) Toxin-Antitoxin Systems in Estuarine Synechococcus Strain CB0101 and Their Transcriptomic Responses to Environmental Stressors. Frontiers in Microbiology 8:1213. [PubMed:28729858]