Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/- |
| Location | 23508..24113 | Replicon | chromosome |
| Accession | MK291442 | ||
| Organism | Paracoccus phage vB_PkoS_Pkon1 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | - |
| Locus tag | pkon1_p44 | Protein ID | AZV00273.1 |
| Coordinates | 23862..24113 (-) | Length | 84 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | - |
| Locus tag | pkon1_p43 | Protein ID | AZV00272.1 |
| Coordinates | 23508..23849 (-) | Length | 114 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| pkon1_p36 | 18611..19357 | + | 747 | AZV00265.1 | structural protein | - |
| pkon1_p37 | 19454..20443 | + | 990 | AZV00266.1 | major capsid protein | - |
| pkon1_p38 | 20436..21302 | + | 867 | AZV00267.1 | hypothetical protein | - |
| pkon1_p39 | 21302..21502 | + | 201 | AZV00268.1 | hypothetical protein | - |
| pkon1_p40 | 21502..21918 | + | 417 | AZV00269.1 | hypothetical protein | - |
| pkon1_p41 | 21918..22268 | + | 351 | AZV00270.1 | hypothetical protein | - |
| pkon1_p42 | 22476..23507 | + | 1032 | AZV00271.1 | head morphogenesis protein | - |
| pkon1_p43 | 23508..23849 | - | 342 | AZV00272.1 | type II toxin-antitoxin HipB-like antitoxin | Antitoxin |
| pkon1_p44 | 23862..24113 | - | 252 | AZV00273.1 | type II toxin-antitoxin RelE-family toxin | Toxin |
| pkon1_p45 | 24188..24604 | + | 417 | AZV00274.1 | tail protein | - |
| pkon1_p46 | 24601..25029 | + | 429 | AZV00275.1 | tail terminator protein | - |
| pkon1_p47 | 25051..25503 | + | 453 | AZV00276.1 | hypothetical protein | - |
| pkon1_p48 | 25506..26042 | + | 537 | AZV00277.1 | hypothetical protein | - |
| pkon1_p49 | 26093..26311 | + | 219 | AZV00278.1 | hypothetical protein | - |
| pkon1_p50 | 26390..27367 | + | 978 | AZV00279.1 | hypothetical protein | - |
| pkon1_p51 | 27471..27728 | - | 258 | AZV00280.1 | Arc-like DNA-binding protein | - |
| pkon1_p52 | 27825..28016 | + | 192 | AZV00281.1 | Arc-family transcriptional repressor | - |
| pkon1_p53 | 28060..28845 | + | 786 | AZV00282.1 | DNA-binding domain (KilA-N) containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Prophage | - | - | 63..46842 | 46779 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 84 a.a. Molecular weight: 9071.50 Da Isoelectric Point: 10.6628
>T10123 AZV00273.1 MK291442:c24113-23862 [Paracoccus phage vB_PkoS_Pkon1]
MKQIAYTKAARKTLSRMPANTSALIRSKIEAYAADPASQANNVKALKGREGIRLRVGDWRVIMDDQGNVLAVLEIGPRGG
IYD
MKQIAYTKAARKTLSRMPANTSALIRSKIEAYAADPASQANNVKALKGREGIRLRVGDWRVIMDDQGNVLAVLEIGPRGG
IYD
Download Length: 252 bp
>T10123 MK291442:c24113-23862 [Paracoccus phage vB_PkoS_Pkon1]
ATGAAGCAGATCGCCTACACCAAAGCCGCACGCAAAACGCTCTCCCGGATGCCTGCCAACACGTCCGCGCTGATCCGGTC
TAAGATCGAAGCTTATGCTGCCGACCCGGCTTCGCAGGCGAACAACGTCAAGGCGCTCAAGGGTCGCGAGGGTATCAGGC
TCCGGGTGGGCGATTGGCGCGTGATTATGGACGACCAAGGCAATGTGCTGGCCGTTCTCGAAATTGGGCCGCGTGGCGGC
ATTTACGACTGA
ATGAAGCAGATCGCCTACACCAAAGCCGCACGCAAAACGCTCTCCCGGATGCCTGCCAACACGTCCGCGCTGATCCGGTC
TAAGATCGAAGCTTATGCTGCCGACCCGGCTTCGCAGGCGAACAACGTCAAGGCGCTCAAGGGTCGCGAGGGTATCAGGC
TCCGGGTGGGCGATTGGCGCGTGATTATGGACGACCAAGGCAATGTGCTGGCCGTTCTCGAAATTGGGCCGCGTGGCGGC
ATTTACGACTGA
Antitoxin
Download Length: 114 a.a. Molecular weight: 12203.85 Da Isoelectric Point: 4.3865
>AT10123 AZV00272.1 MK291442:c23849-23508 [Paracoccus phage vB_PkoS_Pkon1]
MNQMVTIPRDEYDRLLAAAEDLADLQAYDRAMADLASGKEELIPAEYVNRILGGESPVRAFRDLRGMTQAALAEASGVNR
VYIAEIEGGKKAGSAATLKKLAEALHVDLDDLV
MNQMVTIPRDEYDRLLAAAEDLADLQAYDRAMADLASGKEELIPAEYVNRILGGESPVRAFRDLRGMTQAALAEASGVNR
VYIAEIEGGKKAGSAATLKKLAEALHVDLDDLV
Download Length: 342 bp
>AT10123 MK291442:c23849-23508 [Paracoccus phage vB_PkoS_Pkon1]
ATGAACCAGATGGTCACTATCCCCCGTGATGAATACGACCGCCTTCTGGCGGCGGCAGAAGATCTTGCCGACCTGCAAGC
ATATGACCGCGCCATGGCCGATCTGGCGTCCGGCAAGGAAGAGCTGATCCCCGCCGAATATGTGAACCGTATTCTGGGCG
GCGAAAGCCCGGTACGGGCGTTCCGCGATCTTCGCGGCATGACCCAAGCGGCTCTGGCCGAAGCATCCGGCGTAAACCGC
GTGTACATCGCCGAAATCGAAGGTGGCAAGAAAGCTGGTTCTGCTGCTACGCTGAAGAAGCTGGCCGAAGCCCTTCATGT
CGATCTGGACGATCTGGTGTAA
ATGAACCAGATGGTCACTATCCCCCGTGATGAATACGACCGCCTTCTGGCGGCGGCAGAAGATCTTGCCGACCTGCAAGC
ATATGACCGCGCCATGGCCGATCTGGCGTCCGGCAAGGAAGAGCTGATCCCCGCCGAATATGTGAACCGTATTCTGGGCG
GCGAAAGCCCGGTACGGGCGTTCCGCGATCTTCGCGGCATGACCCAAGCGGCTCTGGCCGAAGCATCCGGCGTAAACCGC
GTGTACATCGCCGAAATCGAAGGTGGCAAGAAAGCTGGTTCTGCTGCTACGCTGAAGAAGCTGGCCGAAGCCCTTCATGT
CGATCTGGACGATCTGGTGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T326 | Streptococcus pneumoniae TIGR4 |
34.483 |
100 |
0.361 |
| T727 | Streptococcus pneumoniae R6 |
34.483 |
100 |
0.361 |
| T10191 | Synechococcus sp. CB0101 |
32.911 |
95.181 |
0.313 |
| T10158 | Streptococcus suis SC84 |
29.07 |
100 |
0.301 |
| T1729 | Acidithiobacillus caldus |
29.07 |
100 |
0.301 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|
Antitoxin
| Source | ID | Structure |
|---|
References
(1) Przemyslaw Decewicz et al. (2019) Characterization of the virome of Paracoccus spp. (Alphaproteobacteria) by combined in silico and in vivo approaches. Scientific Reports 9(1):7899. [PubMed:31133656]