Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) pasABC/-
Location 5234..5714 Replicon plasmid pTC-F14
Accession NC_004734
Organism Acidithiobacillus caldus

Toxin (Protein)


Gene name pasB Uniprot ID Q93TU1
Locus tag HKK01_RS00040 Protein ID WP_011110519.1
Coordinates 5442..5714 (+) Length 91 a.a.

Antitoxin (Protein)


Gene name pasA Uniprot ID Q93TU2
Locus tag HKK01_RS00035 Protein ID WP_011110518.1
Coordinates 5234..5458 (+) Length 75 a.a.

Genomic Context

HKK01_RS00025HKK01_RS00030HKK01_RS00035HKK01_RS00040HKK01_RS00045HKK01_RS00050HKK01_RS00055HKK01_RS00015HKK01_RS00020HKK01_RS00060HKK01_RS00090HKK01_RS00065
Location: 2209..2520 (312 bp)
Type: Others
Protein ID: WP_011110525.1
Location: 2510..5179 (2670 bp)
Type: Others
Protein ID: WP_011110526.1
Location: 5234..5458 (225 bp)
Type: Antitoxin
Protein ID: WP_011110518.1
Location: 5442..5714 (273 bp)
Type: Toxin
Protein ID: WP_011110519.1
Location: 5831..6706 (876 bp)
Type: Others
Protein ID: WP_011110520.1
Location: 6788..7681 (894 bp)
Type: Others
Protein ID: WP_168940533.1
Location: 8100..8474 (375 bp)
Type: Others
Protein ID: WP_011110527.1
Location: 852..1532 (681 bp)
Type: Others
Protein ID: WP_011110523.1
Location: 1529..1924 (396 bp)
Type: Others
Protein ID: WP_011110524.1
Location: 8489..9058 (570 bp)
Type: Others
Protein ID: WP_011110528.1
Location: 9156..9341 (186 bp)
Type: Others
Protein ID: Protein_12
Location: 9342..9800 (459 bp)
Type: Others
Protein ID: WP_168940534.1
ToxinAntitoxinOther genes
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Locus tag
Coordinates
Strand
Size (bp)
Protein ID
Product
Description
HKK01_RS00015 (pTC-F14_p02)852..1532-681WP_011110523.1hypothetical protein-
HKK01_RS00020 (pTC-F14_p03)1529..1924-396WP_011110524.1histone-like mobilization protein-
HKK01_RS00025 (pTC-F14_p04)2209..2520+312WP_011110525.1ribbon-helix-helix protein, CopG family-
HKK01_RS00030 (pTC-F14_p05)2510..5179+2670WP_011110526.1TraI/MobA(P) family conjugative relaxase-
HKK01_RS00035 (pTC-F14_p07)5234..5458+225WP_011110518.1DUF6290 family proteinAntitoxin
HKK01_RS00040 (pTC-F14_p08)5442..5714+273WP_011110519.1type II toxin-antitoxin system RelE/ParE family toxinToxin
HKK01_RS00045 (pTC-F14_p09)5831..6706+876WP_011110520.1helicase RepA family protein-
HKK01_RS00050 (pTC-F14_p10)6788..7681+894WP_168940533.1replication protein C, IncQ-type-
HKK01_RS00055 (pTC-F14_p11)8100..8474+375WP_011110527.1DNA-binding protein-
HKK01_RS00060 (pTC-F14_p12)8489..9058-570WP_011110528.1recombinase family protein-
HKK01_RS000909156..9341-186Protein_12base excision DNA repair protein-
HKK01_RS00065 (pTC-F14_p13)9342..9800-459WP_168940534.1carboxymuconolactone decarboxylase family protein-

Associated MGEs

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MGE
detail
Similar
MGEs
Relative
position
MGE Type
Cargo ARG
Virulence gene
Coordinates
Length (bp)
-insideMobilizable plasmid--1..1415514155


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-88)

Antitoxin

Relaxosome protein TraY

(4-49)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10513.21 Da        Isoelectric Point: 10.5753

>T1729 WP_011110519.1 NC_004734:5442-5714 [Acidithiobacillus caldus]
MAWRIEFDDKAKKDLAALDKSVAKRITAFLRERVAHLDDPRSIGEALKGSKLGDFWKYRVGDWRIIASIEDEALRILVVR
IGNRREVYRK

Download         Length: 273 bp

>T1729 NC_004734:5442-5714 [Acidithiobacillus caldus]
TTGGCTTGGCGGATTGAGTTTGATGACAAGGCCAAGAAAGACTTGGCTGCGCTGGATAAGAGCGTTGCCAAACGCATCAC
GGCTTTTCTGCGAGAGCGCGTCGCCCATCTAGACGACCCGCGCAGCATTGGCGAAGCCCTCAAAGGCTCCAAGCTGGGAG
ACTTCTGGAAGTACCGCGTAGGGGATTGGCGAATCATTGCCAGCATTGAGGATGAAGCCTTGCGTATTCTTGTCGTGCGT
ATTGGTAACCGCCGAGAGGTATACCGAAAGTAA

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8532.63 Da        Isoelectric Point: 4.3885

>AT1729 WP_011110518.1 NC_004734:5234-5458 [Acidithiobacillus caldus]
MLAIRLPTEVENRLEALAQATGRTKTFYAREAILEHLDDLEDLYLAEQRLIDIRAGRSRTYTLEEVERDLGLAD

Download         Length: 225 bp

>AT1729 NC_004734:5234-5458 [Acidithiobacillus caldus]
ATGCTTGCCATCCGATTACCCACCGAAGTGGAAAATCGCCTTGAGGCCTTGGCGCAGGCCACAGGACGCACGAAAACCTT
TTACGCCCGCGAAGCGATCCTTGAGCACCTGGACGACCTCGAAGATTTGTACCTTGCAGAGCAACGCCTGATCGACATTC
GCGCAGGCCGAAGCCGTACCTACACGCTAGAGGAAGTGGAGCGCGATCTTGGCTTGGCGGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

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Protein
Organism
Identities (%)
Coverage (%)
Ha-value
T10157Streptococcus suis SC84

38.889

100

0.393

T326Streptococcus pneumoniae TIGR4

40.789

90.476

0.369

T727Streptococcus pneumoniae R6

40.789

90.476

0.369

T10191Synechococcus sp. CB0101

34.831

100

0.369

T10158Streptococcus suis SC84

33.721

100

0.337

T6285Streptomyces cattleya NRRL 8057 = DSM 46488

32.558

98.851

0.322

T10123Paracoccus phage vB_PkoS_Pkon1

29.07

100

0.301
Showing 1 to 7 of 7 rows

Multiple sequence alignment    


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Protein
Organism
Identities (%)
Coverage (%)
Ha-value
No matching records found

Structures

Toxin

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Source
ID
Structure
AlphaFold DBQ93TU1

Antitoxin

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Source
ID
Structure
AlphaFold DBQ93TU2

References

(1) Shelly M Deane et al. (2004) Plasmid evolution and interaction between the plasmid addiction stability systems of two related broad-host-range IncQ-like plasmids. Journal of Bacteriology 186(7):2123-33. [PubMed:15028698] experimental


experimental experimental literature