Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | pasABC/- |
| Location | 5234..5714 | Replicon | plasmid pTC-F14 |
| Accession | NC_004734 | ||
| Organism | Acidithiobacillus caldus | ||
Toxin (Protein)
| Gene name | pasB | Uniprot ID | Q93TU1 |
| Locus tag | HKK01_RS00040 | Protein ID | WP_011110519.1 |
| Coordinates | 5442..5714 (+) | Length | 91 a.a. |
Antitoxin (Protein)
| Gene name | pasA | Uniprot ID | Q93TU2 |
| Locus tag | HKK01_RS00035 | Protein ID | WP_011110518.1 |
| Coordinates | 5234..5458 (+) | Length | 75 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HKK01_RS00015 (pTC-F14_p02) | 852..1532 | - | 681 | WP_011110523.1 | hypothetical protein | - |
| HKK01_RS00020 (pTC-F14_p03) | 1529..1924 | - | 396 | WP_011110524.1 | histone-like mobilization protein | - |
| HKK01_RS00025 (pTC-F14_p04) | 2209..2520 | + | 312 | WP_011110525.1 | ribbon-helix-helix protein, CopG family | - |
| HKK01_RS00030 (pTC-F14_p05) | 2510..5179 | + | 2670 | WP_011110526.1 | TraI/MobA(P) family conjugative relaxase | - |
| HKK01_RS00035 (pTC-F14_p07) | 5234..5458 | + | 225 | WP_011110518.1 | DUF6290 family protein | Antitoxin |
| HKK01_RS00040 (pTC-F14_p08) | 5442..5714 | + | 273 | WP_011110519.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| HKK01_RS00045 (pTC-F14_p09) | 5831..6706 | + | 876 | WP_011110520.1 | helicase RepA family protein | - |
| HKK01_RS00050 (pTC-F14_p10) | 6788..7681 | + | 894 | WP_168940533.1 | replication protein C, IncQ-type | - |
| HKK01_RS00055 (pTC-F14_p11) | 8100..8474 | + | 375 | WP_011110527.1 | DNA-binding protein | - |
| HKK01_RS00060 (pTC-F14_p12) | 8489..9058 | - | 570 | WP_011110528.1 | recombinase family protein | - |
| HKK01_RS00090 | 9156..9341 | - | 186 | Protein_12 | base excision DNA repair protein | - |
| HKK01_RS00065 (pTC-F14_p13) | 9342..9800 | - | 459 | WP_168940534.1 | carboxymuconolactone decarboxylase family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | - | - | 1..14155 | 14155 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 91 a.a. Molecular weight: 10513.21 Da Isoelectric Point: 10.5753
>T1729 WP_011110519.1 NC_004734:5442-5714 [Acidithiobacillus caldus]
MAWRIEFDDKAKKDLAALDKSVAKRITAFLRERVAHLDDPRSIGEALKGSKLGDFWKYRVGDWRIIASIEDEALRILVVR
IGNRREVYRK
MAWRIEFDDKAKKDLAALDKSVAKRITAFLRERVAHLDDPRSIGEALKGSKLGDFWKYRVGDWRIIASIEDEALRILVVR
IGNRREVYRK
Download Length: 273 bp
>T1729 NC_004734:5442-5714 [Acidithiobacillus caldus]
TTGGCTTGGCGGATTGAGTTTGATGACAAGGCCAAGAAAGACTTGGCTGCGCTGGATAAGAGCGTTGCCAAACGCATCAC
GGCTTTTCTGCGAGAGCGCGTCGCCCATCTAGACGACCCGCGCAGCATTGGCGAAGCCCTCAAAGGCTCCAAGCTGGGAG
ACTTCTGGAAGTACCGCGTAGGGGATTGGCGAATCATTGCCAGCATTGAGGATGAAGCCTTGCGTATTCTTGTCGTGCGT
ATTGGTAACCGCCGAGAGGTATACCGAAAGTAA
TTGGCTTGGCGGATTGAGTTTGATGACAAGGCCAAGAAAGACTTGGCTGCGCTGGATAAGAGCGTTGCCAAACGCATCAC
GGCTTTTCTGCGAGAGCGCGTCGCCCATCTAGACGACCCGCGCAGCATTGGCGAAGCCCTCAAAGGCTCCAAGCTGGGAG
ACTTCTGGAAGTACCGCGTAGGGGATTGGCGAATCATTGCCAGCATTGAGGATGAAGCCTTGCGTATTCTTGTCGTGCGT
ATTGGTAACCGCCGAGAGGTATACCGAAAGTAA
Antitoxin
Download Length: 75 a.a. Molecular weight: 8532.63 Da Isoelectric Point: 4.3885
>AT1729 WP_011110518.1 NC_004734:5234-5458 [Acidithiobacillus caldus]
MLAIRLPTEVENRLEALAQATGRTKTFYAREAILEHLDDLEDLYLAEQRLIDIRAGRSRTYTLEEVERDLGLAD
MLAIRLPTEVENRLEALAQATGRTKTFYAREAILEHLDDLEDLYLAEQRLIDIRAGRSRTYTLEEVERDLGLAD
Download Length: 225 bp
>AT1729 NC_004734:5234-5458 [Acidithiobacillus caldus]
ATGCTTGCCATCCGATTACCCACCGAAGTGGAAAATCGCCTTGAGGCCTTGGCGCAGGCCACAGGACGCACGAAAACCTT
TTACGCCCGCGAAGCGATCCTTGAGCACCTGGACGACCTCGAAGATTTGTACCTTGCAGAGCAACGCCTGATCGACATTC
GCGCAGGCCGAAGCCGTACCTACACGCTAGAGGAAGTGGAGCGCGATCTTGGCTTGGCGGATTGA
ATGCTTGCCATCCGATTACCCACCGAAGTGGAAAATCGCCTTGAGGCCTTGGCGCAGGCCACAGGACGCACGAAAACCTT
TTACGCCCGCGAAGCGATCCTTGAGCACCTGGACGACCTCGAAGATTTGTACCTTGCAGAGCAACGCCTGATCGACATTC
GCGCAGGCCGAAGCCGTACCTACACGCTAGAGGAAGTGGAGCGCGATCTTGGCTTGGCGGATTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T10157 | Streptococcus suis SC84 |
38.889 |
100 |
0.393 |
| T326 | Streptococcus pneumoniae TIGR4 |
40.789 |
90.476 |
0.369 |
| T727 | Streptococcus pneumoniae R6 |
40.789 |
90.476 |
0.369 |
| T10191 | Synechococcus sp. CB0101 |
34.831 |
100 |
0.369 |
| T10158 | Streptococcus suis SC84 |
33.721 |
100 |
0.337 |
| T6285 | Streptomyces cattleya NRRL 8057 = DSM 46488 |
32.558 |
98.851 |
0.322 |
| T10123 | Paracoccus phage vB_PkoS_Pkon1 |
29.07 |
100 |
0.301 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
References
(1) Shelly M Deane et al. (2004) Plasmid evolution and interaction between the plasmid addiction stability systems of two related broad-host-range IncQ-like plasmids. Journal of Bacteriology 186(7):2123-33. [PubMed:15028698]