Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/RHH-RelE |
| Location | 1104862..1105420 | Replicon | chromosome |
| Accession | NC_003098 | ||
| Organism | Streptococcus pneumoniae R6 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | B1IC19 |
| Locus tag | SPR_RS05505 | Protein ID | WP_001809704.1 |
| Coordinates | 1104862..1105116 (-) | Length | 85 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | B1IC20 |
| Locus tag | SPR_RS05510 | Protein ID | WP_000208080.1 |
| Coordinates | 1105118..1105360 (-) | Length | 81 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| SPR_RS05500 (spr1102) | 1102916..1104787 | - | 1872 | WP_000150986.1 | Eco57I restriction-modification methylase domain-containing protein | - |
| SPR_RS05505 (spr1103) | 1104862..1105116 | - | 255 | WP_001809704.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| SPR_RS05510 (spr1104) | 1105118..1105360 | - | 243 | WP_000208080.1 | hypothetical protein | Antitoxin |
| SPR_RS05515 (spr1105) | 1105451..1106260 | - | 810 | WP_001289493.1 | MBL fold metallo-hydrolase | - |
| SPR_RS05520 (spr1106) | 1106262..1107611 | - | 1350 | WP_000886210.1 | cell wall metabolism sensor histidine kinase VicK | - |
| SPR_RS05525 (spr1107) | 1107604..1108308 | - | 705 | WP_000722076.1 | response regulator transcription factor | - |
| SPR_RS05530 (spr1108) | 1108363..1109538 | - | 1176 | WP_000886145.1 | A/G-specific adenine glycosylase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 85 a.a. Molecular weight: 10098.79 Da Isoelectric Point: 10.7632
>T727 WP_001809704.1 NC_003098:c1105116-1104862 [Streptococcus pneumoniae R6]
MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEDPRRHGKALVGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHR
RDIY
MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEDPRRHGKALVGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHR
RDIY
Download Length: 255 bp
>T727 NC_003098:c1105116-1104862 [Streptococcus pneumoniae R6]
TTGTATAAATTAGTTCCAACAAGACGTTTCATCAAGCAATTGAAAAAATTGGACCGTTATACGCAGAAGCTAATTACAAA
CTATTTACAAACCAATGTTTTGGAAGACCCAAGACGACACGGAAAGGCTTTGGTTGGTAATCGCGTTGGTCAATGGCGCT
ATAGAATTGGTAATTATCGAGTTATCGTACAAATTGTAGATGATGAATTAGTCGTTGCTACTCTAGAAGTTGGTCATCGG
AGAGATATTTATTGA
TTGTATAAATTAGTTCCAACAAGACGTTTCATCAAGCAATTGAAAAAATTGGACCGTTATACGCAGAAGCTAATTACAAA
CTATTTACAAACCAATGTTTTGGAAGACCCAAGACGACACGGAAAGGCTTTGGTTGGTAATCGCGTTGGTCAATGGCGCT
ATAGAATTGGTAATTATCGAGTTATCGTACAAATTGTAGATGATGAATTAGTCGTTGCTACTCTAGAAGTTGGTCATCGG
AGAGATATTTATTGA
Antitoxin
Download Length: 81 a.a. Molecular weight: 9205.46 Da Isoelectric Point: 4.0527
>AT727 WP_000208080.1 NC_003098:c1105360-1105118 [Streptococcus pneumoniae R6]
MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYDARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE
MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYDARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE
Download Length: 243 bp
>AT727 NC_003098:c1105360-1105118 [Streptococcus pneumoniae R6]
ATGACTACTATTACATTAAAAGTTTCTGAAGCTGATAAAACATTTATGAAAGCAATGGCTAAGTTTGAAGGAGTTTCCCT
TTCGGAACTTATTCGCACCAAAACTCTTGAAGCCCTAGAAGATGAATACGATGCTCGTGTGGCAGATTTAGCCTATCAAG
AGTATTTAGAAGACTTGGAAAAAGGAGTTGAACCCATTACTTGGGAAGAAATGATGCATGATTTAGGCTTGAAGGATGAA
TAA
ATGACTACTATTACATTAAAAGTTTCTGAAGCTGATAAAACATTTATGAAAGCAATGGCTAAGTTTGAAGGAGTTTCCCT
TTCGGAACTTATTCGCACCAAAACTCTTGAAGCCCTAGAAGATGAATACGATGCTCGTGTGGCAGATTTAGCCTATCAAG
AGTATTTAGAAGACTTGGAAAAAGGAGTTGAACCCATTACTTGGGAAGAAATGATGCATGATTTAGGCTTGAAGGATGAA
TAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T326 | Streptococcus pneumoniae TIGR4 |
100 |
100 |
1 |
| T10157 | Streptococcus suis SC84 |
50.588 |
100 |
0.512 |
| T10158 | Streptococcus suis SC84 |
45.349 |
100 |
0.464 |
| T1729 | Acidithiobacillus caldus |
40.789 |
90.476 |
0.369 |
| T10123 | Paracoccus phage vB_PkoS_Pkon1 |
34.483 |
100 |
0.361 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT326 | Streptococcus pneumoniae TIGR4 |
100 |
100 |
1 |
| AT10158 | Streptococcus suis SC84 |
33.766 |
100 |
0.356 |
Multiple sequence alignment
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A853PEW4 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A064C2J7 |
References
(1) Inma Moreno-Córdoba et al. (2012) The toxin-antitoxin proteins relBE2Spn of Streptococcus pneumoniae: characterization and association to their DNA target. Proteins 80(7):1834-46. [PubMed:22488579]
(2) Wai Ting Chan et al. (2018) The Streptococcus pneumoniaeyefM-yoeB and relBE Toxin-Antitoxin Operons Participate in Oxidative Stress and Biofilm Formation. Toxins 10(9):378. [PubMed:30231554]
(3) Inmaculada Moreno-Córdoba et al. (2021) Interactions of the Streptococcus pneumoniae Toxin-Antitoxin RelBE Proteins with Their Target DNA. Microorganisms 9(4):851. [PubMed:33921033]