Detailed information of TA system TA000326

Experimentally validated

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH-RelE
• Location: 1156713..1157271
• Replicon: chromosome
• Accession: NC_003028
• Organism: Streptococcus pneumoniae TIGR4

Toxin (Protein)

• Gene name: relE
• Uniprot ID: B1IC19
• Locus tag: SP_RS05995
• Protein ID: WP_001809704.1
• Coordinates: 1156713..1156967 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: B1IC20
• Locus tag: SP_RS06000
• Protein ID: WP_000208080.1
• Coordinates: 1156969..1157211 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SP_RS05990 (SP_1222)	1154767..1156638	-	1872	WP_000150986.1	Eco57I restriction-modification methylase domain-containing protein	-
SP_RS05995 (SP_1223)	1156713..1156967	-	255	WP_001809704.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SP_RS06000 (SP_1224)	1156969..1157211	-	243	WP_000208080.1	hypothetical protein	Antitoxin
SP_RS06005 (SP_1225)	1157302..1158111	-	810	WP_001289493.1	MBL fold metallo-hydrolase	-
SP_RS06010 (SP_1226)	1158113..1159462	-	1350	WP_000886219.1	cell wall metabolism sensor histidine kinase VicK	-
SP_RS06015 (SP_1227)	1159455..1160159	-	705	WP_000722076.1	response regulator transcription factor	-
SP_RS06020 (SP_1228)	1160215..1161390	-	1176	WP_000886146.1	A/G-specific adenine glycosylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (7-84)

Antitoxin

• Protein of unknown function DUF6290 (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10098.79 Da
• Isoelectric Point: 10.7632

>T326 WP_001809704.1 NC_003028:c1156967-1156713 [Streptococcus pneumoniae TIGR4]
MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEDPRRHGKALVGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHR
RDIY

Nucleotide

• Length: 255 bp

>T326 NC_003028:c1156967-1156713 [Streptococcus pneumoniae TIGR4]
TTGTATAAATTAGTTCCAACAAGACGTTTTATCAAGCAATTGAAAAAATTGGACCGTTATACGCAGAAGCTAATTACAAA
CTATTTACAAACCAATGTTTTGGAAGACCCAAGACGACACGGAAAGGCTTTGGTTGGTAATCGCGTTGGTCAATGGCGCT
ATAGAATTGGTAATTATCGAGTTATCGTACAAATTGTAGATGATGAATTAGTCGTTGCTACTCTAGAAGTTGGTCATCGG
AGAGATATTTATTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9205.46 Da
• Isoelectric Point: 4.0527

>AT326 WP_000208080.1 NC_003028:c1157211-1156969 [Streptococcus pneumoniae TIGR4]
MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYDARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE

Nucleotide

• Length: 243 bp

>AT326 NC_003028:c1157211-1156969 [Streptococcus pneumoniae TIGR4]
ATGACTACTATTACATTAAAAGTTTCTGAAGCTGATAAAACATTTATGAAAGCAATGGCTAAGTTTGAAGGAGTTTCCCT
TTCGGAACTTATTCGCACCAAAACTCTTGAAGCCCTAGAAGATGAATACGATGCTCGTGTGGCAGATTTAGCCTATCAAG
AGTATTTAGAAGACTTGGAAAAAGGAGTTGAACCCATTACTTGGGAAGAAATGATGCATGATTTAGGCTTGAAGGATGAA
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Toxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
T727	Streptococcus pneumoniae R6	100	100	1
T10157	Streptococcus suis SC84	50.588	100	0.512
T10158	Streptococcus suis SC84	45.349	100	0.464
T1729	Acidithiobacillus caldus	40.789	90.476	0.369
T10123	Paracoccus phage vB_PkoS_Pkon1	34.483	100	0.361

Multiple sequence alignment

Antitoxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
AT727	Streptococcus pneumoniae R6	100	100	1
AT10158	Streptococcus suis SC84	33.766	100	0.356

Multiple sequence alignment

Structures

Toxin

• AlphaFold DB: A0A853PEW4

Antitoxin

• AlphaFold DB: A0A064C2J7

References

(1) Concha Nieto et al. (2010) The relBE2Spn toxin-antitoxin system of Streptococcus pneumoniae: role in antibiotic tolerance and functional conservation in clinical isolates. PloS One 5(6):e11289. [PubMed:20585658]


 experimental literature