Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/RelE(toxin) |
| Location | 4216503..4217028 | Replicon | chromosome |
| Accession | NC_017586 | ||
| Organism | Streptomyces cattleya NRRL 8057 = DSM 46488 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | F8K3H9 |
| Locus tag | SCATT_RS18610 | Protein ID | WP_014144656.1 |
| Coordinates | 4216503..4216766 (-) | Length | 88 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | G8WSR4 |
| Locus tag | SCATT_RS18615 | Protein ID | WP_014628308.1 |
| Coordinates | 4216759..4217028 (-) | Length | 90 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| SCATT_RS18590 (SCATT_39240) | 4211682..4213037 | + | 1356 | WP_014144652.1 | hypothetical protein | - |
| SCATT_RS18595 (SCATT_39250) | 4213039..4213569 | + | 531 | WP_014144653.1 | NUDIX domain-containing protein | - |
| SCATT_RS18600 | 4213700..4214527 | + | 828 | WP_014144654.1 | TIGR04255 family protein | - |
| SCATT_RS18605 (SCATT_39260) | 4215233..4216195 | + | 963 | WP_157894840.1 | hypothetical protein | - |
| SCATT_RS18610 (SCATT_39270) | 4216503..4216766 | - | 264 | WP_014144656.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| SCATT_RS18615 (SCATT_39280) | 4216759..4217028 | - | 270 | WP_014628308.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | Antitoxin |
| SCATT_RS18620 (SCATT_39290) | 4217210..4218628 | + | 1419 | WP_014144658.1 | ATP-binding protein | - |
| SCATT_RS18625 (SCATT_39310) | 4218749..4220461 | + | 1713 | WP_014142185.1 | IS1182 family transposase | - |
| SCATT_RS38640 (SCATT_39320) | 4220586..4220939 | + | 354 | WP_014144659.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| inside | Integrative and Conjugative Element | - | - | 4200177..4241727 | 41550 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 88 a.a. Molecular weight: 9968.42 Da Isoelectric Point: 9.5565
>T6285 WP_014144656.1 NC_017586:c4216766-4216503 [Streptomyces cattleya NRRL 8057 = DSM 46488]
VSEYRTVFRPEAQAELRKVPRDMALRILAKLTELESDPLGFNTTALVSQPDRRRLRVGDYRVIYTIDNGELVVWVVHVGH
RSTVYGA
VSEYRTVFRPEAQAELRKVPRDMALRILAKLTELESDPLGFNTTALVSQPDRRRLRVGDYRVIYTIDNGELVVWVVHVGH
RSTVYGA
Download Length: 264 bp
>T6285 NC_017586:c4216766-4216503 [Streptomyces cattleya NRRL 8057 = DSM 46488]
GTGAGTGAGTACCGCACCGTCTTCCGACCCGAGGCTCAGGCCGAACTCCGCAAGGTCCCGCGCGACATGGCTCTCCGCAT
TCTGGCCAAGCTGACCGAGCTGGAGAGCGACCCGCTGGGATTCAACACCACTGCTCTCGTCTCCCAGCCCGACCGGCGCC
GCCTACGGGTCGGCGACTACCGCGTGATCTACACGATCGACAATGGTGAGCTGGTGGTGTGGGTCGTGCATGTCGGGCAT
CGCTCAACCGTCTACGGCGCCTGA
GTGAGTGAGTACCGCACCGTCTTCCGACCCGAGGCTCAGGCCGAACTCCGCAAGGTCCCGCGCGACATGGCTCTCCGCAT
TCTGGCCAAGCTGACCGAGCTGGAGAGCGACCCGCTGGGATTCAACACCACTGCTCTCGTCTCCCAGCCCGACCGGCGCC
GCCTACGGGTCGGCGACTACCGCGTGATCTACACGATCGACAATGGTGAGCTGGTGGTGTGGGTCGTGCATGTCGGGCAT
CGCTCAACCGTCTACGGCGCCTGA
Antitoxin
Download Length: 90 a.a. Molecular weight: 10322.79 Da Isoelectric Point: 4.7473
>AT6285 WP_014628308.1 NC_017586:c4217028-4216759 [Streptomyces cattleya NRRL 8057 = DSM 46488]
MTQPLPMESIRDVRAHLAEVVERADRDDVPTVITRRGKQVAAVVSIEVLRKYQEWEEREINRIIDERMANPTPGVPIEDI
MRETLARSE
MTQPLPMESIRDVRAHLAEVVERADRDDVPTVITRRGKQVAAVVSIEVLRKYQEWEEREINRIIDERMANPTPGVPIEDI
MRETLARSE
Download Length: 270 bp
>AT6285 NC_017586:c4217028-4216759 [Streptomyces cattleya NRRL 8057 = DSM 46488]
ATGACACAACCGCTGCCCATGGAGTCCATCCGCGATGTCCGGGCACACCTCGCCGAGGTCGTGGAGCGGGCTGACCGCGA
CGACGTGCCCACGGTGATCACGCGCCGAGGCAAGCAGGTGGCTGCCGTGGTGTCCATCGAAGTGCTCCGGAAGTACCAGG
AGTGGGAAGAGCGCGAGATCAACCGAATCATCGATGAGCGCATGGCCAACCCGACACCCGGCGTCCCGATCGAGGACATC
ATGAGGGAGACGCTGGCGCGCAGTGAGTGA
ATGACACAACCGCTGCCCATGGAGTCCATCCGCGATGTCCGGGCACACCTCGCCGAGGTCGTGGAGCGGGCTGACCGCGA
CGACGTGCCCACGGTGATCACGCGCCGAGGCAAGCAGGTGGCTGCCGTGGTGTCCATCGAAGTGCTCCGGAAGTACCAGG
AGTGGGAAGAGCGCGAGATCAACCGAATCATCGATGAGCGCATGGCCAACCCGACACCCGGCGTCCCGATCGAGGACATC
ATGAGGGAGACGCTGGCGCGCAGTGAGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T10191 | Synechococcus sp. CB0101 |
40 |
95.238 |
0.381 |
| T124 | Mycobacterium tuberculosis H37Rv |
31.461 |
100 |
0.322 |
| T10157 | Streptococcus suis SC84 |
32.184 |
100 |
0.322 |
| T1729 | Acidithiobacillus caldus |
32.558 |
98.851 |
0.322 |
| T93 | Mycobacterium tuberculosis H37Rv |
31.818 |
100 |
0.322 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT172 | Vibrio cholerae O1 biovar El Tor str. N16961 |
31.395 |
96.629 |
0.303 |
Multiple sequence alignment
References
(1) Peng Li et al. (2016) Identification and characterization of chromosomal relBE toxin-antitoxin locus in Streptomyces cattleya DSM46488. Scientific Reports 6:32047. [PubMed:27534445]