Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/PHD-RelE |
| Location | 3177537..3178085 | Replicon | chromosome |
| Accession | NC_000962 | ||
| Organism | Mycobacterium tuberculosis H37Rv | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | O33348 |
| Locus tag | Rv2866 | Protein ID | NP_217382.1 |
| Coordinates | 3177822..3178085 (+) | Length | 88 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | O33347 |
| Locus tag | Rv2865 | Protein ID | NP_217381.1 |
| Coordinates | 3177537..3177818 (+) | Length | 94 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| Rv2861c | 3173160..3174017 | - | 858 | YP_177911.1 | methionine aminopeptidase | - |
| Rv2862c | 3174059..3174643 | - | 585 | NP_217378.1 | hypothetical protein | - |
| Rv2862A | 3174747..3174995 | + | 249 | YP_007411570.1 | antitoxin VapB23 | - |
| Rv2863 | 3174992..3175372 | + | 381 | NP_217379.1 | ribonuclease VapC23 | - |
| Rv2864c | 3175454..3177265 | - | 1812 | NP_217380.1 | penicillin-binding lipoprotein | - |
| Rv2865 | 3177537..3177818 | + | 282 | NP_217381.1 | antitoxin RelF | Antitoxin |
| Rv2866 | 3177822..3178085 | + | 264 | NP_217382.1 | toxin RelG | Toxin |
| Rv2867c | 3178458..3179312 | - | 855 | NP_217383.1 | GCN5-like N-acetyltransferase | - |
| Rv2868c | 3179368..3180531 | - | 1164 | NP_217384.1 | 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) | - |
| Rv2869c | 3180548..3181762 | - | 1215 | NP_217385.1 | zinc metalloprotease | - |
| Rv2870c | 3181770..3183011 | - | 1242 | NP_217386.2 | 1-deoxy-D-xylulose 5-phosphate reductoisomerase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 88 a.a. Molecular weight: 10204.82 Da Isoelectric Point: 11.5078
>T124 NP_217382.1 NC_000962:3177822-3178085 [Mycobacterium tuberculosis H37Rv]
VPYTVRFTTTARRDLHKLPPRILAAVVEFAFGDLSREPLRVGKPLRRELAGTFSARRGTYRLLYRIDDEHTTVVILRVDH
RADIYRR
VPYTVRFTTTARRDLHKLPPRILAAVVEFAFGDLSREPLRVGKPLRRELAGTFSARRGTYRLLYRIDDEHTTVVILRVDH
RADIYRR
Download Length: 264 bp
>T124 NC_000962:3177822-3178085 [Mycobacterium tuberculosis H37Rv]
GTGCCTTACACCGTGCGGTTCACCACAACCGCGCGTCGAGACCTCCACAAGCTGCCACCGCGCATCCTCGCGGCAGTGGT
CGAATTCGCGTTCGGCGATCTGTCGCGCGAGCCCCTGCGGGTGGGCAAGCCCCTTCGGCGCGAGTTGGCCGGCACGTTCA
GCGCGCGTCGCGGAACGTACCGCCTGCTGTACCGGATTGACGACGAGCACACAACGGTAGTGATCCTGCGCGTCGATCAC
CGCGCGGACATCTACCGCCGATAG
GTGCCTTACACCGTGCGGTTCACCACAACCGCGCGTCGAGACCTCCACAAGCTGCCACCGCGCATCCTCGCGGCAGTGGT
CGAATTCGCGTTCGGCGATCTGTCGCGCGAGCCCCTGCGGGTGGGCAAGCCCCTTCGGCGCGAGTTGGCCGGCACGTTCA
GCGCGCGTCGCGGAACGTACCGCCTGCTGTACCGGATTGACGACGAGCACACAACGGTAGTGATCCTGCGCGTCGATCAC
CGCGCGGACATCTACCGCCGATAG
Antitoxin
Download Length: 94 a.a. Molecular weight: 10183.40 Da Isoelectric Point: 4.3440
>AT124 NP_217381.1 NC_000962:3177537-3177818 [Mycobacterium tuberculosis H37Rv]
MRILPISTIKGKLNEFVDAVSSTQDQITITKNGAPAAVLVGADEWESLQETLYWLAQPGIRESIAEADADIASGRTYGED
EIRAEFGVPRRPH
MRILPISTIKGKLNEFVDAVSSTQDQITITKNGAPAAVLVGADEWESLQETLYWLAQPGIRESIAEADADIASGRTYGED
EIRAEFGVPRRPH
Download Length: 282 bp
>AT124 NC_000962:3177537-3177818 [Mycobacterium tuberculosis H37Rv]
ATGCGGATACTGCCGATTTCGACGATCAAGGGCAAGCTCAATGAGTTCGTCGACGCGGTCTCGTCGACACAGGACCAGAT
CACCATCACCAAGAACGGTGCACCCGCAGCCGTTCTGGTCGGCGCCGACGAGTGGGAATCGTTGCAGGAGACGCTGTACT
GGCTGGCGCAACCCGGAATCAGGGAGTCGATCGCTGAAGCCGACGCCGACATTGCCTCCGGCCGCACCTACGGCGAAGAC
GAGATCCGCGCCGAATTCGGCGTCCCGCGACGCCCCCACTGA
ATGCGGATACTGCCGATTTCGACGATCAAGGGCAAGCTCAATGAGTTCGTCGACGCGGTCTCGTCGACACAGGACCAGAT
CACCATCACCAAGAACGGTGCACCCGCAGCCGTTCTGGTCGGCGCCGACGAGTGGGAATCGTTGCAGGAGACGCTGTACT
GGCTGGCGCAACCCGGAATCAGGGAGTCGATCGCTGAAGCCGACGCCGACATTGCCTCCGGCCGCACCTACGGCGAAGAC
GAGATCCGCGCCGAATTCGGCGTCCCGCGACGCCCCCACTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T93 | Mycobacterium tuberculosis H37Rv |
54.118 |
97.701 |
0.529 |
| T6285 | Streptomyces cattleya NRRL 8057 = DSM 46488 |
31.461 |
100 |
0.322 |
| T10158 | Streptococcus suis SC84 |
29.885 |
100 |
0.302 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT93 | Mycobacterium tuberculosis H37Rv |
44.186 |
96.629 |
0.427 |
Multiple sequence alignment
References
(1) Shaleen B Korch et al. (2009) Three Mycobacterium tuberculosis Rel toxin-antitoxin modules inhibit mycobacterial growth and are expressed in infected human macrophages. Journal of Bacteriology 191(5):1618-30. [PubMed:19114484]