Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/PHD-RelE |
| Location | 1388685..1389244 | Replicon | chromosome |
| Accession | NC_000962 | ||
| Organism | Mycobacterium tuberculosis H37Rv | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | O50461 |
| Locus tag | Rv1246c | Protein ID | NP_215762.1 |
| Coordinates | 1388685..1388978 (-) | Length | 98 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | O50462 |
| Locus tag | Rv1247c | Protein ID | NP_215763.1 |
| Coordinates | 1388975..1389244 (-) | Length | 90 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| Rv1241 | 1384278..1384538 | + | 261 | NP_215757.1 | antitoxin VapB33 | - |
| Rv1242 | 1384535..1384966 | + | 432 | NP_215758.1 | ribonuclease VapC33 | - |
| Rv1243c | 1384989..1386677 | - | 1689 | YP_177798.1 | PE-PGRS family protein PE_PGRS23 | - |
| Rv1244 | 1386857..1387717 | + | 861 | NP_215760.1 | lipoprotein LpqZ | - |
| Rv1245c | 1387798..1388628 | - | 831 | NP_215761.1 | short-chain type dehydrogenase/reductase | - |
| Rv1246c | 1388685..1388978 | - | 294 | NP_215762.1 | toxin RelE | Toxin |
| Rv1247c | 1388975..1389244 | - | 270 | NP_215763.1 | antitoxin RelB | Antitoxin |
| Rv1248c | 1389357..1393052 | - | 3696 | NP_215764.2 | multifunctional 2-oxoglutarate dehydrogenase E1 component /2-oxoglutarate dehydrogenase dihydrolipoyllysine-residue succinyltransferase | - |
| Rv1249c | 1393194..1393982 | - | 789 | NP_215765.1 | membrane protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 98 a.a. Molecular weight: 11031.57 Da Isoelectric Point: 9.6275
>T93 NP_215762.1 NC_000962:c1388978-1388685 [Mycobacterium tuberculosis H37Rv]
VSDDHPYHVAITATAARDLQRLPEKIAAACVEFVFGPLLNNPHRLGKPLRNDLEGLHSARRGDYRVVYAIDDGHHRVEII
HIARRSASYRMNPCRPR
VSDDHPYHVAITATAARDLQRLPEKIAAACVEFVFGPLLNNPHRLGKPLRNDLEGLHSARRGDYRVVYAIDDGHHRVEII
HIARRSASYRMNPCRPR
Download Length: 294 bp
>T93 NC_000962:c1388978-1388685 [Mycobacterium tuberculosis H37Rv]
GTGAGCGACGACCATCCCTACCACGTGGCGATCACCGCGACAGCGGCACGCGACCTGCAACGCTTACCCGAAAAGATCGC
CGCCGCATGTGTCGAGTTTGTTTTCGGACCGCTGCTTAACAACCCGCATAGGTTGGGCAAGCCGCTGCGCAATGACCTTG
AAGGCCTCCACTCAGCCCGCCGCGGTGATTACCGCGTCGTCTACGCCATCGACGACGGCCACCACCGAGTCGAGATCATC
CACATCGCTCGTCGCAGTGCCAGCTACCGAATGAACCCGTGCCGGCCACGTTAA
GTGAGCGACGACCATCCCTACCACGTGGCGATCACCGCGACAGCGGCACGCGACCTGCAACGCTTACCCGAAAAGATCGC
CGCCGCATGTGTCGAGTTTGTTTTCGGACCGCTGCTTAACAACCCGCATAGGTTGGGCAAGCCGCTGCGCAATGACCTTG
AAGGCCTCCACTCAGCCCGCCGCGGTGATTACCGCGTCGTCTACGCCATCGACGACGGCCACCACCGAGTCGAGATCATC
CACATCGCTCGTCGCAGTGCCAGCTACCGAATGAACCCGTGCCGGCCACGTTAA
Antitoxin
Download Length: 90 a.a. Molecular weight: 9766.97 Da Isoelectric Point: 4.8615
>AT93 NP_215763.1 NC_000962:c1389244-1388975 [Mycobacterium tuberculosis H37Rv]
MAVVPLGEVRNRLSEYVAEVELTHERITITRHGHPAAVLISADDLASIEETLEVLRTPGASEAIREGLADVAAGRFVSND
EIRNRYTAR
MAVVPLGEVRNRLSEYVAEVELTHERITITRHGHPAAVLISADDLASIEETLEVLRTPGASEAIREGLADVAAGRFVSND
EIRNRYTAR
Download Length: 270 bp
>AT93 NC_000962:c1389244-1388975 [Mycobacterium tuberculosis H37Rv]
ATGGCTGTTGTCCCACTGGGCGAAGTCCGCAATCGCCTCTCTGAGTACGTCGCCGAAGTTGAGCTGACACACGAGCGCAT
CACGATAACCCGGCACGGTCATCCGGCGGCGGTATTGATCTCGGCCGATGACCTGGCGTCCATCGAGGAAACGCTGGAGG
TGCTACGCACCCCTGGCGCCAGCGAGGCCATTCGTGAAGGCCTCGCCGATGTTGCCGCAGGGCGCTTCGTGAGCAACGAC
GAGATCCGCAACCGTTACACCGCGCGGTGA
ATGGCTGTTGTCCCACTGGGCGAAGTCCGCAATCGCCTCTCTGAGTACGTCGCCGAAGTTGAGCTGACACACGAGCGCAT
CACGATAACCCGGCACGGTCATCCGGCGGCGGTATTGATCTCGGCCGATGACCTGGCGTCCATCGAGGAAACGCTGGAGG
TGCTACGCACCCCTGGCGCCAGCGAGGCCATTCGTGAAGGCCTCGCCGATGTTGCCGCAGGGCGCTTCGTGAGCAACGAC
GAGATCCGCAACCGTTACACCGCGCGGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T124 | Mycobacterium tuberculosis H37Rv |
54.118 |
97.701 |
0.529 |
| T6285 | Streptomyces cattleya NRRL 8057 = DSM 46488 |
31.818 |
100 |
0.322 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT124 | Mycobacterium tuberculosis H37Rv |
44.186 |
96.629 |
0.427 |
| AT130 | Mycobacterium tuberculosis H37Rv |
35.714 |
94.382 |
0.337 |
Multiple sequence alignment
References
(1) Min Yang et al. (2010) Characterization of the interaction and cross-regulation of three Mycobacterium tuberculosis RelBE modules. PloS One 5(5):e10672. [PubMed:20498855]
(2) Shaleen B Korch et al. (2009) Three Mycobacterium tuberculosis Rel toxin-antitoxin modules inhibit mycobacterial growth and are expressed in infected human macrophages. Journal of Bacteriology 191(5):1618-30. [PubMed:19114484]