Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) relBE/PHD-RelE
Location 3770773..3771302 Replicon chromosome
Accession NC_000962
Organism Mycobacterium tuberculosis H37Rv

Toxin (Protein)


Gene name relE Uniprot ID P9WF09
Locus tag Rv3358 Protein ID NP_217875.1
Coordinates 3771045..3771302 (+) Length 86 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID P9WF25
Locus tag Rv3357 Protein ID NP_217874.1
Coordinates 3770773..3771048 (+) Length 92 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Rv3351c 3767346..3768140 - 795 NP_217868.1 hypothetical protein -
Rv3352c 3768222..3768593 - 372 NP_217869.1 oxidoreductase -
Rv3353c 3768736..3768996 - 261 NP_217870.2 hypothetical protein -
Rv3354 3769111..3769500 + 390 NP_217871.1 hypothetical protein -
Rv3355c 3769514..3769807 - 294 NP_217872.1 integral membrane protein -
Rv3356c 3769804..3770649 - 846 NP_217873.1 bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase -
Rv3357 3770773..3771048 + 276 NP_217874.1 antitoxin RelJ Antitoxin
Rv3358 3771045..3771302 + 258 NP_217875.1 toxin RelK Toxin
Rv3359 3771344..3772534 + 1191 NP_217876.1 oxidoreductase -
Rv3360 3772651..3773019 + 369 NP_217877.1 hypothetical protein -
Rv3361c 3773016..3773567 - 552 NP_217878.1 hypothetical protein -
Rv3362c 3773574..3774155 - 582 NP_217879.1 ATP/GTP-binding protein -
Rv3363c 3774136..3774504 - 369 NP_217880.1 hypothetical protein -
Rv3364c 3774482..3774874 - 393 NP_217881.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(6-85)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(2-72)


Sequences

Toxin        


Download         Length: 86 a.a.        Molecular weight: 10070.30 Da        Isoelectric Point: 6.4693

>T130 NP_217875.1 NC_000962:3771045-3771302 [Mycobacterium tuberculosis H37Rv]
VRSVNFDPDAWEDFLFWLAADRKTARRITRLIGEIQRDPFSGIGKPEPLQGELSGYWSRRIDDEHRLVYRAGDDEVTMLK
ARYHY

Download         Length: 258 bp

>T130 NC_000962:3771045-3771302 [Mycobacterium tuberculosis H37Rv]
GTGAGAAGCGTCAACTTCGATCCCGATGCCTGGGAGGACTTCTTGTTCTGGCTGGCCGCTGATCGCAAAACGGCCCGTCG
GATCACCCGGTTGATCGGAGAAATTCAGCGTGATCCGTTCAGCGGGATCGGCAAACCCGAGCCGCTCCAAGGTGAGTTGT
CGGGATACTGGTCGCGCCGGATCGACGACGAACACCGGCTGGTGTATCGAGCGGGCGACGACGAAGTCACGATGCTGAAG
GCCCGATACCACTACTGA

Antitoxin


Download         Length: 92 a.a.        Molecular weight: 10194.35 Da        Isoelectric Point: 4.6652

>AT130 NP_217874.1 NC_000962:3770773-3771048 [Mycobacterium tuberculosis H37Rv]
MSISASEARQRLFPLIEQVNTDHQPVRITSRAGDAVLMSADDYDAWQETVYLLRSPENARRLMEAVARDKAGHSAFTKSV
DELREMAGGEE

Download         Length: 276 bp

>AT130 NC_000962:3770773-3771048 [Mycobacterium tuberculosis H37Rv]
ATGAGCATCAGTGCGAGCGAGGCGAGGCAGCGCCTGTTTCCACTCATCGAACAGGTCAATACCGATCACCAGCCGGTGCG
GATCACCTCCCGGGCCGGCGATGCGGTGCTGATGTCCGCCGACGACTACGACGCGTGGCAGGAAACGGTCTATCTGCTGC
GCTCACCGGAGAACGCCAGGCGGTTGATGGAAGCGGTTGCCCGGGATAAGGCTGGGCACTCGGCTTTCACCAAGTCTGTA
GATGAGCTGCGGGAGATGGCCGGCGGCGAGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T6122 Streptomyces coelicolor A3(2)

59.756

97.619

0.583
T310 Pseudomonas putida KT2440

54.217

97.647

0.529
T728 Streptococcus pneumoniae R6

52.439

97.619

0.512
T4918 Actinobacillus pleuropneumoniae serovar 3 str. JL03

51.22

96.471

0.494
T6281 Streptococcus suis SC84

48.78

96.471

0.471
T1043 Escherichia coli str. K-12 substr. MG1655

49.351

91.667

0.452
T6115 Enterococcus faecium

48.718

91.765

0.447
T6022 Enterococcus faecium

48.718

91.765

0.447
T10021 Lacticaseibacillus rhamnosus 51B

47.436

91.765

0.435
T10020 Lacticaseibacillus rhamnosus strain 24

47.436

91.765

0.435
T6138 Lactobacillus rhamnosus Lc 705

47.436

91.765

0.435
T6143 Lactobacillus rhamnosus ATCC 8530

47.436

91.765

0.435
T6237 Lactobacillus rhamnosus Lc 705

47.436

91.765

0.435
T10072 Edwardsiella tarda EIB202

42.857

98.824

0.424
T613 Staphylococcus aureus subsp. aureus MW2

41.667

98.824

0.412
T2427 Staphylococcus aureus subsp. aureus NCTC 8325

41.667

98.824

0.412
T10077 Agrobacterium tumefaciens str. C58

43.21

95.294

0.412
T1074 Staphylococcus aureus subsp. aureus N315

40.476

98.824

0.4
T10182 Aggregatibacter actinomycetemcomitans D11S 1

45.07

85.542

0.386

Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value
AT6122 Streptomyces coelicolor A3(2)

70.833

82.759

0.586
AT10182 Aggregatibacter actinomycetemcomitans D11S 1

57.895

90.476

0.524
AT1043 Escherichia coli str. K-12 substr. MG1655

50

86.747

0.434
AT4918 Actinobacillus pleuropneumoniae serovar 3 str. JL03

43.836

87.952

0.386
AT2427 Staphylococcus aureus subsp. aureus NCTC 8325

38.961

92.771

0.361
AT1074 Staphylococcus aureus subsp. aureus N315

38.961

92.771

0.361
AT613 Staphylococcus aureus subsp. aureus MW2

38.961

92.771

0.361
AT310 Pseudomonas putida KT2440

38.75

87.912

0.341
AT93 Mycobacterium tuberculosis H37Rv

35.714

94.382

0.337
AT10072 Edwardsiella tarda EIB202

34.722

88.889

0.309
AT6022 Enterococcus faecium

34.177

88.764

0.303
AT6115 Enterococcus faecium

34.177

88.764

0.303

Multiple sequence alignment    




Structures

Toxin

Source ID Structure
PDB 3OEI

Antitoxin

Source ID Structure
PDB 3D55
PDB 3CTO
PDB 3OEI
AlphaFold DB P9WF25

References

(1) Shaleen B Korch et al. (2009) Three Mycobacterium tuberculosis Rel toxin-antitoxin modules inhibit mycobacterial growth and are expressed in infected human macrophages. Journal of Bacteriology 191(5):1618-30. [PubMed:19114484] experimental


experimental experimental literature