Detailed information of TA system TA000130

Experimentally validated

Overview

TA module

• Type: II
• Classification (family/domain): relBE/PHD-RelE
• Location: 3770773..3771302
• Replicon: chromosome
• Accession: NC_000962
• Organism: Mycobacterium tuberculosis H37Rv

Toxin (Protein)

• Gene name: relE
• Uniprot ID: P9WF09
• Locus tag: Rv3358
• Protein ID: NP_217875.1
• Coordinates: 3771045..3771302 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: P9WF25
• Locus tag: Rv3357
• Protein ID: NP_217874.1
• Coordinates: 3770773..3771048 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Rv3351c	3767346..3768140	-	795	NP_217868.1	hypothetical protein	-
Rv3352c	3768222..3768593	-	372	NP_217869.1	oxidoreductase	-
Rv3353c	3768736..3768996	-	261	NP_217870.2	hypothetical protein	-
Rv3354	3769111..3769500	+	390	NP_217871.1	hypothetical protein	-
Rv3355c	3769514..3769807	-	294	NP_217872.1	integral membrane protein	-
Rv3356c	3769804..3770649	-	846	NP_217873.1	bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase	-
Rv3357	3770773..3771048	+	276	NP_217874.1	antitoxin RelJ	Antitoxin
Rv3358	3771045..3771302	+	258	NP_217875.1	toxin RelK	Toxin
Rv3359	3771344..3772534	+	1191	NP_217876.1	oxidoreductase	-
Rv3360	3772651..3773019	+	369	NP_217877.1	hypothetical protein	-
Rv3361c	3773016..3773567	-	552	NP_217878.1	hypothetical protein	-
Rv3362c	3773574..3774155	-	582	NP_217879.1	ATP/GTP-binding protein	-
Rv3363c	3774136..3774504	-	369	NP_217880.1	hypothetical protein	-
Rv3364c	3774482..3774874	-	393	NP_217881.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (6-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10070.30 Da
• Isoelectric Point: 6.4693

>T130 NP_217875.1 NC_000962:3771045-3771302 [Mycobacterium tuberculosis H37Rv]
VRSVNFDPDAWEDFLFWLAADRKTARRITRLIGEIQRDPFSGIGKPEPLQGELSGYWSRRIDDEHRLVYRAGDDEVTMLK
ARYHY

Nucleotide

• Length: 258 bp

>T130 NC_000962:3771045-3771302 [Mycobacterium tuberculosis H37Rv]
GTGAGAAGCGTCAACTTCGATCCCGATGCCTGGGAGGACTTCTTGTTCTGGCTGGCCGCTGATCGCAAAACGGCCCGTCG
GATCACCCGGTTGATCGGAGAAATTCAGCGTGATCCGTTCAGCGGGATCGGCAAACCCGAGCCGCTCCAAGGTGAGTTGT
CGGGATACTGGTCGCGCCGGATCGACGACGAACACCGGCTGGTGTATCGAGCGGGCGACGACGAAGTCACGATGCTGAAG
GCCCGATACCACTACTGA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10194.35 Da
• Isoelectric Point: 4.6652

>AT130 NP_217874.1 NC_000962:3770773-3771048 [Mycobacterium tuberculosis H37Rv]
MSISASEARQRLFPLIEQVNTDHQPVRITSRAGDAVLMSADDYDAWQETVYLLRSPENARRLMEAVARDKAGHSAFTKSV
DELREMAGGEE

Nucleotide

• Length: 276 bp

>AT130 NC_000962:3770773-3771048 [Mycobacterium tuberculosis H37Rv]
ATGAGCATCAGTGCGAGCGAGGCGAGGCAGCGCCTGTTTCCACTCATCGAACAGGTCAATACCGATCACCAGCCGGTGCG
GATCACCTCCCGGGCCGGCGATGCGGTGCTGATGTCCGCCGACGACTACGACGCGTGGCAGGAAACGGTCTATCTGCTGC
GCTCACCGGAGAACGCCAGGCGGTTGATGGAAGCGGTTGCCCGGGATAAGGCTGGGCACTCGGCTTTCACCAAGTCTGTA
GATGAGCTGCGGGAGATGGCCGGCGGCGAGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Toxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
T6122	Streptomyces coelicolor A3(2)	59.756	97.619	0.583
T310	Pseudomonas putida KT2440	54.217	97.647	0.529
T728	Streptococcus pneumoniae R6	52.439	97.619	0.512
T4918	Actinobacillus pleuropneumoniae serovar 3 str. JL03	51.22	96.471	0.494
T6281	Streptococcus suis SC84	48.78	96.471	0.471
T1043	Escherichia coli str. K-12 substr. MG1655	49.351	91.667	0.452
T6115	Enterococcus faecium	48.718	91.765	0.447
T6022	Enterococcus faecium	48.718	91.765	0.447
T10021	Lacticaseibacillus rhamnosus 51B	47.436	91.765	0.435
T10020	Lacticaseibacillus rhamnosus strain 24	47.436	91.765	0.435
T6138	Lactobacillus rhamnosus Lc 705	47.436	91.765	0.435
T6143	Lactobacillus rhamnosus ATCC 8530	47.436	91.765	0.435
T6237	Lactobacillus rhamnosus Lc 705	47.436	91.765	0.435
T10072	Edwardsiella tarda EIB202	42.857	98.824	0.424
T613	Staphylococcus aureus subsp. aureus MW2	41.667	98.824	0.412
T2427	Staphylococcus aureus subsp. aureus NCTC 8325	41.667	98.824	0.412
T10077	Agrobacterium tumefaciens str. C58	43.21	95.294	0.412
T1074	Staphylococcus aureus subsp. aureus N315	40.476	98.824	0.4
T10182	Aggregatibacter actinomycetemcomitans D11S 1	45.07	85.542	0.386

Multiple sequence alignment

Antitoxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
AT6122	Streptomyces coelicolor A3(2)	70.833	82.759	0.586
AT10182	Aggregatibacter actinomycetemcomitans D11S 1	57.895	90.476	0.524
AT1043	Escherichia coli str. K-12 substr. MG1655	50	86.747	0.434
AT4918	Actinobacillus pleuropneumoniae serovar 3 str. JL03	43.836	87.952	0.386
AT2427	Staphylococcus aureus subsp. aureus NCTC 8325	38.961	92.771	0.361
AT1074	Staphylococcus aureus subsp. aureus N315	38.961	92.771	0.361
AT613	Staphylococcus aureus subsp. aureus MW2	38.961	92.771	0.361
AT310	Pseudomonas putida KT2440	38.75	87.912	0.341
AT93	Mycobacterium tuberculosis H37Rv	35.714	94.382	0.337
AT10072	Edwardsiella tarda EIB202	34.722	88.889	0.309
AT6022	Enterococcus faecium	34.177	88.764	0.303
AT6115	Enterococcus faecium	34.177	88.764	0.303

Multiple sequence alignment

Structures

Toxin

• PDB: 3OEI

Antitoxin

• AlphaFold DB: P9WF25

References

(1) Shaleen B Korch et al. (2009) Three Mycobacterium tuberculosis Rel toxin-antitoxin modules inhibit mycobacterial growth and are expressed in infected human macrophages. Journal of Bacteriology 191(5):1618-30. [PubMed:19114484]


 experimental literature