1074

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/PHD-RelE
Location 2466001..2466518 Replicon chromosome
Accession NC_002745
Organism Staphylococcus aureus subsp. aureus N315

Toxin (Protein)


Gene name yoeB Uniprot ID A0A3A3ATA4
Locus tag SA_RS12585 Protein ID WP_000113265.1
Coordinates 2466001..2466267 (-) Length 89 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID T1YB60
Locus tag SA_RS12590 Protein ID WP_000584499.1
Coordinates 2466267..2466518 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SA_RS12560 (SA2190) 2461429..2461965 - 537 WP_000169317.1 GNAT family N-acetyltransferase -
SA_RS12565 (SA2191) 2462199..2463023 - 825 WP_000572038.1 formate/nitrite transporter family protein -
SA_RS12570 (SA2192) 2463240..2463422 - 183 WP_000230292.1 hypothetical protein -
SA_RS12575 (SA2193) 2463506..2463973 - 468 WP_000153347.1 SRPBCC domain-containing protein -
SA_RS12580 (SA2194) 2464159..2465706 - 1548 WP_000727772.1 zinc ABC transporter substrate-binding lipoprotein AdcA -
SA_RS14935 2465895..2465966 - 72 Protein_2358 hypothetical protein -
SA_RS12585 (SA2195) 2466001..2466267 - 267 WP_000113265.1 Txe/YoeB family addiction module toxin Toxin
SA_RS12590 (SA2196) 2466267..2466518 - 252 WP_000584499.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
SA_RS12595 2466683..2466784 - 102 Protein_2361 hypothetical protein -
SA_RS12600 (SA2197) 2466791..2467390 - 600 WP_000162810.1 DsbA family protein -
SA_RS12605 (SA2198) 2467409..2467771 - 363 WP_000819843.1 DUF4467 domain-containing protein -
SA_RS12610 (SA2199) 2468026..2469276 + 1251 WP_001012225.1 FemA/FemB family glycyltransferase FmhA -
SA_RS12615 (SA2200) 2469373..2470104 - 732 WP_000615461.1 amino acid ABC transporter ATP-binding protein -
SA_RS12620 2470101..2470820 - 720 WP_000479576.1 amino acid ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-81)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-74)


Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10436.96 Da        Isoelectric Point: 10.0573

>T1074 WP_000113265.1 NC_002745:c2466267-2466001 [Staphylococcus aureus subsp. aureus N315]
MARLNITFSPQAFEDYKYFQQNNKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Download         Length: 267 bp

>T1074 NC_002745:c2466267-2466001 [Staphylococcus aureus subsp. aureus N315]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACAATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9433.60 Da        Isoelectric Point: 4.3407

>AT1074 WP_000584499.1 NC_002745:c2466518-2466267 [Staphylococcus aureus subsp. aureus N315]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Download         Length: 252 bp

>AT1074 NC_002745:c2466518-2466267 [Staphylococcus aureus subsp. aureus N315]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T2427 Staphylococcus aureus subsp. aureus NCTC 8325

98.864

100

0.989
T613 Staphylococcus aureus subsp. aureus MW2

98.864

100

0.989
T4918 Actinobacillus pleuropneumoniae serovar 3 str. JL03

54.118

98.837

0.535
T1043 Escherichia coli str. K-12 substr. MG1655

51.765

100

0.524
T6022 Enterococcus faecium

50.602

97.647

0.494
T6115 Enterococcus faecium

50.602

97.647

0.494
T10021 Lacticaseibacillus rhamnosus 51B

46.988

96.512

0.453
T10020 Lacticaseibacillus rhamnosus strain 24

46.988

96.512

0.453
T6138 Lactobacillus rhamnosus Lc 705

46.988

96.512

0.453
T6143 Lactobacillus rhamnosus ATCC 8530

46.988

96.512

0.453
T6237 Lactobacillus rhamnosus Lc 705

46.988

96.512

0.453
T728 Streptococcus pneumoniae R6

44.578

98.81

0.44
T6122 Streptomyces coelicolor A3(2)

43.529

100

0.44
T6281 Streptococcus suis SC84

43.529

100

0.435
T130 Mycobacterium tuberculosis H37Rv

40.476

98.824

0.4
T10072 Edwardsiella tarda EIB202

37.079

100

0.375
T310 Pseudomonas putida KT2440

38.372

97.727

0.375
T10077 Agrobacterium tumefaciens str. C58

35.955

100

0.364
T10182 Aggregatibacter actinomycetemcomitans D11S 1

34.568

97.59

0.337

Multiple sequence alignment    



Structures

Toxin

Source ID Structure
PDB 7BWF

Antitoxin

Source ID Structure
AlphaFold DB T1YB60

References

(1) Niles P Donegan et al. (2010) Proteolytic regulation of toxin-antitoxin systems by ClpPC in Staphylococcus aureus. Journal of Bacteriology 192(5):1416-22. [PubMed:20038589] experimental

(2) Hyun-Jong Eun et al. (2020) Crystal structure of the YoeBSa1-YefMSa1 complex from Staphylococcus aureus. Biochemical And Biophysical Research Communications 527(1):264-269. [PubMed:32446378] 3D_structure


experimental experimental literature
3D_structure protein structure literature