Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) relBE/PHD-RelE
Location 1417602..1418110 Replicon chromosome
Accession NC_010278
Organism Actinobacillus pleuropneumoniae serovar 3 str. JL03

Toxin (Protein)


Gene name relE Uniprot ID B0BQK9
Locus tag APJL_1288 Protein ID WP_005605064.1
Coordinates 1417850..1418110 (+) Length 87 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID B0BQK8
Locus tag APJL_1287 Protein ID WP_005598318.1
Coordinates 1417602..1417853 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
APJL_RS06335 (APJL_1284) 1413137..1413685 - 549 WP_012263163.1 type IV pilus biogenesis/stability protein PilW -
APJL_RS06340 (APJL_1285) 1413739..1414920 - 1182 WP_005608495.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
APJL_RS06365 (APJL_1286) 1415825..1417264 + 1440 WP_005605060.1 glutamate--tRNA ligase -
APJL_RS06375 (APJL_1287) 1417602..1417853 + 252 WP_005598318.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
APJL_RS06380 (APJL_1288) 1417850..1418110 + 261 WP_005605064.1 Txe/YoeB family addiction module toxin Toxin
APJL_RS06385 (APJL_1289) 1418268..1418435 - 168 WP_009875287.1 Trm112 family protein -
APJL_RS06390 (APJL_1290) 1418437..1419417 - 981 WP_005608502.1 tetraacyldisaccharide 4'-kinase -
APJL_RS06395 (APJL_1291) 1419511..1420770 - 1260 WP_005598324.1 ATP-dependent protease ATP-binding subunit ClpX -
APJL_RS06400 (APJL_1292) 1420770..1421360 - 591 WP_005598326.1 ATP-dependent Clp endopeptidase proteolytic subunit ClpP -
APJL_RS06405 (APJL_1293) 1421484..1421876 + 393 WP_012263165.1 SufE family protein -
APJL_RS06420 (APJL_1294) 1422235..1423008 - 774 WP_012263166.1 DNA polymerase III subunit epsilon -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 87 a.a.        Molecular weight: 10423.99 Da        Isoelectric Point: 8.0109

>T4918 WP_005605064.1 NC_010278:1417850-1418110 [Actinobacillus pleuropneumoniae serovar 3 str. JL03]
MKLTFSSNAWEDYLYWQKTDKIILKRINSLIKDIQRQPFEGIGKLEPLKFNLSGFWSRRINEEHRLIYSVEDEAILIVAC
RYHYDQ

Download         Length: 261 bp

>T4918 NC_010278:1417850-1418110 [Actinobacillus pleuropneumoniae serovar 3 str. JL03]
ATGAAGCTAACTTTCTCGTCTAATGCGTGGGAAGATTATTTATACTGGCAAAAAACCGATAAAATAATTTTGAAACGTAT
AAACAGCCTGATCAAAGATATTCAACGCCAACCATTCGAAGGCATAGGAAAACTGGAGCCGCTAAAATTCAATCTTTCGG
GATTTTGGTCAAGGCGAATCAATGAAGAACACCGCTTAATTTACAGCGTGGAAGATGAAGCAATTTTAATAGTAGCGTGT
AGATACCATTATGATCAGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9362.66 Da        Isoelectric Point: 4.5659

>AT4918 WP_005598318.1 NC_010278:1417602-1417853 [Actinobacillus pleuropneumoniae serovar 3 str. JL03]
MQAITYTEARQNLAGTMSKVAEDFEPILITRSKGGNCVLMSYEQYCSLEETAYLMRSPTNAKRLLDSVAELRQGQGIERE
LIE

Download         Length: 252 bp

>AT4918 NC_010278:1417602-1417853 [Actinobacillus pleuropneumoniae serovar 3 str. JL03]
ATGCAAGCAATAACCTACACAGAAGCACGCCAAAATCTAGCCGGTACGATGTCAAAAGTAGCAGAAGATTTTGAGCCTAT
TTTAATTACAAGAAGCAAGGGCGGAAATTGCGTACTTATGTCGTATGAGCAGTATTGTTCACTTGAAGAAACCGCCTATT
TGATGCGTTCACCCACCAATGCAAAACGGCTATTGGATAGTGTGGCGGAATTAAGACAGGGACAAGGCATTGAAAGAGAG
CTAATCGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T1043 Escherichia coli str. K-12 substr. MG1655

69.048

100

0.69
T6022 Enterococcus faecium

57.647

100

0.576
T6115 Enterococcus faecium

57.647

100

0.576
T6281 Streptococcus suis SC84

57.143

98.824

0.565
T728 Streptococcus pneumoniae R6

55.952

100

0.56
T6122 Streptomyces coelicolor A3(2)

54.762

100

0.548
T2427 Staphylococcus aureus subsp. aureus NCTC 8325

55.294

98.837

0.547
T613 Staphylococcus aureus subsp. aureus MW2

55.294

98.837

0.547
T6237 Lactobacillus rhamnosus Lc 705

54.118

98.837

0.535
T6143 Lactobacillus rhamnosus ATCC 8530

54.118

98.837

0.535
T6138 Lactobacillus rhamnosus Lc 705

54.118

98.837

0.535
T10021 Lacticaseibacillus rhamnosus 51B

54.118

98.837

0.535
T10020 Lacticaseibacillus rhamnosus strain 24

54.118

98.837

0.535
T1074 Staphylococcus aureus subsp. aureus N315

54.118

98.837

0.535
T310 Pseudomonas putida KT2440

52.941

98.837

0.523
T130 Mycobacterium tuberculosis H37Rv

51.22

96.471

0.494
T10182 Aggregatibacter actinomycetemcomitans D11S 1

48.193

100

0.482
T10077 Agrobacterium tumefaciens str. C58

44.318

100

0.453
T10072 Edwardsiella tarda EIB202

40.964

96.512

0.395

Multiple sequence alignment    



Structures

Toxin

Source ID Structure
AlphaFold DB B0BQK9

Antitoxin

Source ID Structure
AlphaFold DB B0BQK8

References

(1) Chengkun Zheng et al. (2017) Identification of four type II toxin-antitoxin systems in Actinobacillus pleuropneumoniae. FEMS Microbiology Letters 364(12):10.1093/femsle/fnx126. [PubMed:28637172] experimental


experimental experimental literature