Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | yefM-yoeB (relBE)/PHD-RelE |
| Location | 2087235..2087737 | Replicon | chromosome |
| Accession | NC_000913 | ||
| Organism | Escherichia coli str. K-12 substr. MG1655 | ||
Toxin (Protein)
| Gene name | yoeB | Uniprot ID | P69348 |
| Locus tag | b4539 | Protein ID | YP_588458.1 |
| Coordinates | 2089211..2089465 (-) | Length | 85 a.a. |
Antitoxin (Protein)
| Gene name | yefM | Uniprot ID | P69346 |
| Locus tag | b2017 | Protein ID | NP_416521.2 |
| Coordinates | 2089462..2089713 (-) | Length | 84 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| b2011 | 2082756..2084183 | + | 1428 | NP_416515.1 | exodeoxyribonuclease I | - |
| b2012 | 2084226..2084453 | - | 228 | NP_416516.1 | putative thiosulfate sulfurtransferase | - |
| b2013 | 2084467..2085525 | - | 1059 | NP_416517.1 | thiosulfate transporter | - |
| b2014 | 2085704..2087062 | - | 1359 | NP_416518.2 | putrescine:H(+) symporter PlaP | - |
| b4678 | 2087052..2087114 | - | 63 | YP_002791246.1 | uncharacterized protein YoeI | - |
| b2015 | 2087329..2088258 | - | 930 | NP_416519.4 | putative DNA-binding transcriptional regulator YeeY | - |
| b2016 | 2088304..2089128 | - | 825 | NP_416520.1 | putative epimerase YeeZ | - |
| b4539 | 2089211..2089465 | - | 255 | YP_588458.1 | ribosome-dependent mRNA interferase toxin YoeB | Toxin |
| b2017 | 2089462..2089713 | - | 252 | NP_416521.2 | YefM antitoxin of the YoeB-YefM toxin-antitoxin pair and DNA binding transcriptional repressor | Antitoxin |
| b2018 | 2089996..2090046 | + | 51 | NP_416522.1 | his operon leader peptide | - |
| b2019 | 2090192..2091091 | + | 900 | NP_416523.1 | ATP phosphoribosyltransferase | - |
| b2020 | 2091097..2092401 | + | 1305 | NP_416524.1 | histidinal/histidinol dehydrogenase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 85 a.a. Molecular weight: 10183.58 Da Isoelectric Point: 8.0353
>T1043 YP_588458.1 NC_000913:c2089465-2089211 [Escherichia coli str. K-12 substr. MG1655]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY
Download Length: 255 bp
>T1043 NC_000913:c2089465-2089211 [Escherichia coli str. K-12 substr. MG1655]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA
Antitoxin
Download Length: 84 a.a. Molecular weight: 9307.58 Da Isoelectric Point: 4.7954
>AT1043 NP_416521.2 NC_000913:c2089713-2089462 [Escherichia coli str. K-12 substr. MG1655]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE
Download Length: 252 bp
>AT1043 NC_000913:c2089713-2089462 [Escherichia coli str. K-12 substr. MG1655]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA
Similar Proteins
Only experimentally validated proteins are listed.
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT10182 | Aggregatibacter actinomycetemcomitans D11S 1 |
63.415 |
97.619 |
0.619 |
| AT4918 | Actinobacillus pleuropneumoniae serovar 3 str. JL03 |
61.446 |
100 |
0.614 |
| AT6122 | Streptomyces coelicolor A3(2) |
51.852 |
97.59 |
0.506 |
| AT310 | Pseudomonas putida KT2440 |
45.679 |
97.59 |
0.446 |
| AT130 | Mycobacterium tuberculosis H37Rv |
50 |
86.747 |
0.434 |
| AT613 | Staphylococcus aureus subsp. aureus MW2 |
44.156 |
92.771 |
0.41 |
| AT2427 | Staphylococcus aureus subsp. aureus NCTC 8325 |
44.156 |
92.771 |
0.41 |
| AT1074 | Staphylococcus aureus subsp. aureus N315 |
44.156 |
92.771 |
0.41 |
| AT10077 | Agrobacterium tumefaciens str. C58 |
40.964 |
100 |
0.41 |
| AT10072 | Edwardsiella tarda EIB202 |
31.25 |
98.765 |
0.309 |
Multiple sequence alignment
References
(1) Yonglong Zhang et al. (2009) The inhibitory mechanism of protein synthesis by YoeB, an Escherichia coli toxin. The Journal of Biological Chemistry 284(11):6627-38. [PubMed:19124462]
(2) J Paul Norton et al. (2012) Toxin-antitoxin systems are important for niche-specific colonization and stress resistance of uropathogenic Escherichia coli. PLoS Pathogens 8(10):e1002954. [PubMed:23055930]