10072

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/-
Location 1725106..1725607 Replicon chromosome
Accession NC_013508
Organism Edwardsiella tarda EIB202

Toxin (Protein)


Gene name yoeB Uniprot ID -
Locus tag ETAE_RS17330 Protein ID WP_080514633.1
Coordinates 1725332..1725607 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID D0Z7P3
Locus tag ETAE_RS07650 Protein ID WP_012848468.1
Coordinates 1725106..1725351 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ETAE_RS07620 1721332..1722024 - 693 WP_041692511.1 helix-turn-helix transcriptional regulator -
ETAE_RS17320 (ETAE_1615) 1722129..1722344 + 216 WP_012848463.1 helix-turn-helix domain-containing protein -
ETAE_RS07625 (ETAE_1616) 1722442..1722798 + 357 WP_012848464.1 hypothetical protein -
ETAE_RS07630 (ETAE_1617) 1722795..1723028 + 234 WP_005286423.1 hypothetical protein -
ETAE_RS07635 (ETAE_1618) 1723060..1723311 + 252 WP_012848465.1 hypothetical protein -
ETAE_RS07640 (ETAE_1619) 1723514..1724230 + 717 Protein_1518 helix-turn-helix domain-containing protein -
ETAE_RS18200 1724228..1724512 + 285 Protein_1519 DUF1367 family protein -
ETAE_RS07645 (ETAE_1620) 1724515..1724817 + 303 WP_012848467.1 DUF1364 domain-containing protein -
ETAE_RS07650 (ETAE_1621) 1725106..1725351 + 246 WP_012848468.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
ETAE_RS17330 1725332..1725607 + 276 WP_080514633.1 Txe/YoeB family addiction module toxin Toxin
ETAE_RS07655 (ETAE_1622) 1725690..1726202 - 513 WP_012848469.1 DUF2442 domain-containing protein -
ETAE_RS07660 (ETAE_1623) 1726297..1726605 - 309 WP_005286394.1 helix-turn-helix transcriptional regulator -
ETAE_RS07665 1726595..1726924 - 330 WP_015871332.1 type II toxin-antitoxin system RelE/ParE family toxin -
ETAE_RS07670 (ETAE_1625) 1727345..1727992 + 648 Protein_1526 hypothetical protein -
ETAE_RS07675 (ETAE_1626) 1728164..1728502 + 339 WP_012848472.1 phage holin, lambda family -
ETAE_RS07680 (ETAE_1627) 1728505..1729056 + 552 WP_012848473.1 glycoside hydrolase family 108 protein -
ETAE_RS07685 (ETAE_1628) 1729192..1730028 + 837 WP_080514635.1 antA/AntB antirepressor family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1698905..1744957 46052


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-88)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-74)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10528.93 Da        Isoelectric Point: 10.1403

>T10072 WP_080514633.1 NC_013508:1725332-1725607 [Edwardsiella tarda EIB202]
MSQWTINFTLNGAADFAYWKGIDQNKVERIRTLLHSIEADPFSGIGKPERLRHHKNPALYSRRIDQQHRLVYSVSGNTVT
VVACRFHYGDK

Download         Length: 276 bp

>T10072 NC_013508:1725332-1725607 [Edwardsiella tarda EIB202]
TTGTCACAGTGGACTATTAATTTTACCCTAAACGGCGCCGCTGATTTTGCCTACTGGAAGGGGATTGATCAGAACAAGGT
TGAGCGGATACGAACCCTGTTACATTCCATCGAGGCCGACCCGTTCAGTGGGATAGGAAAGCCTGAACGCTTACGCCACC
ACAAGAACCCTGCTCTATACTCGCGCCGTATCGATCAACAGCACCGCCTCGTTTACAGTGTGTCAGGTAACACGGTTACG
GTGGTTGCCTGCCGCTTTCACTACGGGGATAAATGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9157.27 Da        Isoelectric Point: 4.5494

>AT10072 WP_012848468.1 NC_013508:1725106-1725351 [Edwardsiella tarda EIB202]
MHTVTFSDARKHFADTMNRVTNNAEPVRVMRRDAPDIVMVDAGEYEAMLETIYLFSNPANAAHLNESLAQLERGEVVTVD
Y

Download         Length: 246 bp

>AT10072 NC_013508:1725106-1725351 [Edwardsiella tarda EIB202]
ATGCACACTGTTACCTTCTCGGATGCCCGCAAGCATTTTGCGGATACCATGAACCGTGTAACCAACAACGCCGAACCGGT
TCGCGTTATGCGCCGCGATGCACCCGATATTGTGATGGTCGATGCCGGTGAATATGAGGCTATGCTGGAGACGATCTACC
TGTTCAGCAACCCGGCGAACGCGGCCCATCTCAACGAATCATTGGCACAGCTGGAGCGCGGCGAAGTTGTCACAGTGGAC
TATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T130 Mycobacterium tuberculosis H37Rv

42.857

98.824

0.424
T6143 Lactobacillus rhamnosus ATCC 8530

42.169

96.512

0.407
T6138 Lactobacillus rhamnosus Lc 705

42.169

96.512

0.407
T10021 Lacticaseibacillus rhamnosus 51B

42.169

96.512

0.407
T10020 Lacticaseibacillus rhamnosus strain 24

42.169

96.512

0.407
T6237 Lactobacillus rhamnosus Lc 705

42.169

96.512

0.407
T728 Streptococcus pneumoniae R6

40.964

98.81

0.405
T6122 Streptomyces coelicolor A3(2)

40.964

98.81

0.405
T4918 Actinobacillus pleuropneumoniae serovar 3 str. JL03

40.964

96.512

0.395
T613 Staphylococcus aureus subsp. aureus MW2

38.202

100

0.386
T2427 Staphylococcus aureus subsp. aureus NCTC 8325

38.202

100

0.386
T10077 Agrobacterium tumefaciens str. C58

40

95.506

0.382
T1074 Staphylococcus aureus subsp. aureus N315

37.079

100

0.375
T310 Pseudomonas putida KT2440

39.535

94.505

0.374
T1043 Escherichia coli str. K-12 substr. MG1655

41.892

88.095

0.369
T6281 Streptococcus suis SC84

36.145

97.647

0.353
T6115 Enterococcus faecium

36

88.235

0.318
T6022 Enterococcus faecium

36

88.235

0.318
T1075 Staphylococcus aureus subsp. aureus N315

29.032

100

0.307

Multiple sequence alignment    



Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A411H7Q5

References

(1) Dongmei Ma et al. (2021) Edwardsiella piscicida YefM-YoeB: A Type II Toxin-Antitoxin System That Is Related to Antibiotic Resistance, Biofilm Formation, Serum Survival, and Host Infection. Frontiers in Microbiology 12:646299. [PubMed:33732226] experimental


experimental experimental literature