6022

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) Txe-Axe/-
Location 4963..5482 Replicon plasmid pRUM
Accession NC_005000
Organism Enterococcus faecium

Toxin (Protein)


Gene name Txe Uniprot ID Q848U6
Locus tag HKK39_RS00035 Protein ID WP_000588503.1
Coordinates 4963..5220 (-) Length 86 a.a.

Antitoxin (Protein)


Gene name Axe Uniprot ID Q83WJ8
Locus tag HKK39_RS00040 Protein ID WP_000388479.1
Coordinates 5213..5482 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HKK39_RS00005 (pRUM_p01) 1..291 - 291 WP_000026576.1 hypothetical protein -
HKK39_RS00010 427..606 - 180 WP_001021554.1 hypothetical protein -
HKK39_RS00015 (pRUM_p02) 603..953 - 351 WP_000568378.1 hypothetical protein -
HKK39_RS00020 (pRUM_p03) 946..2271 - 1326 WP_000969590.1 Y-family DNA polymerase -
HKK39_RS00025 (pRUM_p04) 2721..3335 + 615 WP_001261742.1 recombinase family protein -
HKK39_RS00030 (pRUM_p05) 3500..4504 - 1005 WP_000455809.1 hypothetical protein -
HKK39_RS00035 (pRUM_p06) 4963..5220 - 258 WP_000588503.1 Txe/YoeB family addiction module toxin Toxin
HKK39_RS00040 (pRUM_p07) 5213..5482 - 270 WP_000388479.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
HKK39_RS00045 5613..5792 - 180 WP_001120991.1 hypothetical protein -
HKK39_RS00180 5808..6008 - 201 WP_001033544.1 4-hydroxybenzoate octaprenyltransferase -
HKK39_RS00050 (pRUM_p10) 6173..6868 - 696 WP_002326819.1 hypothetical protein -
HKK39_RS00055 (pRUM_p11) 6930..7535 + 606 Protein_11 IS6-like element IS1216 family transposase -
HKK39_RS00060 7536..7974 - 439 Protein_12 replication protein RepA -
HKK39_RS00065 7954..8139 - 186 Protein_13 APH(3') family aminoglycoside O-phosphotransferase -
HKK39_RS00070 (pRUM_p14) 8232..8774 - 543 WP_000627290.1 streptothricin N-acetyltransferase Sat4 -
HKK39_RS00075 (pRUM_p16) 8771..9679 - 909 WP_001255866.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -
HKK39_RS00080 (pRUM_p17) 9712..10446 - 735 WP_000662263.1 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Mobilizable plasmid ant(6)-Ia / erm(B) / cat(pC233) - 1..24873 24873


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-85)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-72)


Sequences

Toxin        


Download         Length: 86 a.a.        Molecular weight: 10206.53 Da        Isoelectric Point: 8.4369

>T6022 WP_000588503.1 NC_005000:c5220-4963 [Enterococcus faecium]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY

Download         Length: 258 bp

>T6022 NC_005000:c5220-4963 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10373.51 Da        Isoelectric Point: 4.4346

>AT6022 WP_000388479.1 NC_005000:c5482-5213 [Enterococcus faecium]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD

Download         Length: 270 bp

>AT6022 NC_005000:c5482-5213 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
AT6115 Enterococcus faecium

100

100

1
AT10182 Aggregatibacter actinomycetemcomitans D11S 1

56.471

100

0.571
AT6138 Lactobacillus rhamnosus Lc 705

57.831

93.258

0.539
AT10021 Lacticaseibacillus rhamnosus 51B

57.831

93.258

0.539
AT10020 Lacticaseibacillus rhamnosus strain 24

57.831

93.258

0.539
AT6237 Lactobacillus rhamnosus Lc 705

57.831

93.258

0.539
AT6143 Lactobacillus rhamnosus ATCC 8530

57.831

93.258

0.539
AT6281 Streptococcus suis SC84

45.238

97.674

0.442
AT728 Streptococcus pneumoniae R6

46.25

95.238

0.44
AT2427 Staphylococcus aureus subsp. aureus NCTC 8325

37.662

92.771

0.349
AT1074 Staphylococcus aureus subsp. aureus N315

37.662

92.771

0.349
AT613 Staphylococcus aureus subsp. aureus MW2

37.662

92.771

0.349
AT10077 Agrobacterium tumefaciens str. C58

37.333

89.286

0.333
AT130 Mycobacterium tuberculosis H37Rv

34.177

88.764

0.303

Multiple sequence alignment    




Structures

Toxin

Source ID Structure
AlphaFold DB A0A828ZXQ8

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZWU7

References

(1) Ruth Grady et al. (2003) Axe-Txe, a broad-spectrum proteic toxin-antitoxin system specified by a multidrug-resistant, clinical isolate of Enterococcus faecium. Molecular Microbiology 47(5):1419-32. [PubMed:12603745] experimental

(2) Elizabeth M Halvorsen et al. (2011) Txe, an endoribonuclease of the enterococcal Axe-Txe toxin-antitoxin system, cleaves mRNA and inhibits protein synthesis. Microbiology (Reading, England) 157(Pt 2):387-397. [PubMed:21030436] experimental

(3) Torill C S Rosvoll et al. (2010) PCR-based plasmid typing in Enterococcus faecium strains reveals widely distributed pRE25-, pRUM-, pIP501- and pHTbeta-related replicons associated with glycopeptide resistance and stabilizing toxin-antitoxin systems. FEMS Immunology And Medical Microbiology 58(2):254-68. [PubMed:20015231]


experimental experimental literature