10077

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/-
Location 1979333..1979853 Replicon chromosome
Accession NC_003062
Organism Agrobacterium tumefaciens str. C58

Toxin (Protein)


Gene name yoeB Uniprot ID A9CID9
Locus tag ATU_RS09850 Protein ID WP_010972002.1
Coordinates 1979333..1979602 (-) Length 90 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID Q7CY23
Locus tag ATU_RS09855 Protein ID WP_010972003.1
Coordinates 1979599..1979853 (-) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ATU_RS09830 (AGR_C_3651) 1974364..1976058 - 1695 WP_010971998.1 iron ABC transporter permease -
ATU_RS09835 (AGR_C_3652) 1976068..1977051 - 984 WP_010971999.1 ABC transporter substrate-binding protein -
ATU_RS09840 (AGR_C_3654) 1977360..1978604 - 1245 WP_010972000.1 PLP-dependent aminotransferase family protein -
ATU_RS09845 (AGR_C_3656) 1978879..1979328 + 450 WP_010972001.1 RbsD/FucU family protein -
ATU_RS09850 (AGR_C_3658) 1979333..1979602 - 270 WP_010972002.1 Txe/YoeB family addiction module toxin Toxin
ATU_RS09855 (AGR_C_3659) 1979599..1979853 - 255 WP_010972003.1 type II toxin-antitoxin system prevent-host-death family antitoxin Antitoxin
ATU_RS09860 (AGR_C_3660) 1979992..1981011 - 1020 WP_010972004.1 ketol-acid reductoisomerase -
ATU_RS09865 (AGR_C_3662) 1981059..1981712 - 654 WP_006311841.1 TetR/AcrR family transcriptional regulator -
ATU_RS09870 (AGR_C_3663) 1982112..1983017 - 906 WP_010972005.1 AraC family transcriptional regulator -
ATU_RS09875 (AGR_C_3663A) 1983207..1984253 + 1047 WP_006311839.1 NAD(P)-dependent alcohol dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-88)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-74)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10612.09 Da        Isoelectric Point: 9.8742

>T10077 WP_010972002.1 NC_003062:c1979602-1979333 [Agrobacterium fabrum str. C58]
MKLVWTLSSWDDYEFWQRTDARMVEKINDLIRNAKRTPFAGLGKPEPLKGDMAGYWSRRITAEHRFVYRVSGSGSEQRLE
VIQCRFHYQ

Download         Length: 270 bp

>T10077 NC_003062:c1979602-1979333 [Agrobacterium fabrum str. C58]
ATGAAGCTCGTCTGGACGCTGAGTTCATGGGACGACTATGAGTTCTGGCAAAGAACCGATGCTCGCATGGTCGAGAAAAT
AAACGATCTTATCCGAAATGCCAAACGCACGCCCTTTGCAGGGCTTGGGAAACCGGAGCCTCTGAAGGGCGATATGGCAG
GATATTGGTCTCGGCGGATTACCGCCGAGCATCGGTTTGTCTACCGTGTATCCGGGTCCGGAAGCGAGCAACGGCTGGAA
GTCATTCAGTGCCGCTTCCATTACCAATAA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9577.70 Da        Isoelectric Point: 4.6749

>AT10077 WP_010972003.1 NC_003062:c1979853-1979599 [Agrobacterium fabrum str. C58]
MANVRFTEFRQNFATHFDRVLETRAPLLVTRQGKEAVVVLAEGEYESMQETLHLLSNPANASRLRASMGELERGDTIERD
PTEE

Download         Length: 255 bp

>AT10077 NC_003062:c1979853-1979599 [Agrobacterium fabrum str. C58]
ATGGCGAATGTGCGATTTACCGAGTTCAGGCAGAACTTCGCGACCCATTTCGACCGGGTTCTGGAAACCCGCGCGCCATT
GCTCGTCACGCGGCAAGGCAAAGAGGCGGTCGTGGTGCTTGCCGAAGGAGAATATGAGAGCATGCAGGAAACGCTGCATC
TCCTGTCCAACCCGGCAAATGCCTCAAGGCTTCGCGCGTCCATGGGCGAACTTGAGCGCGGTGACACCATCGAGCGGGAT
CCGACCGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.


Structures

Toxin

Source ID Structure
PDB 6N90

Antitoxin

Source ID Structure
AlphaFold DB Q7CY23

References

(1) Jessica R Ames et al. (2020) Identifying a Molecular Mechanism That Imparts Species-Specific Toxicity to YoeB Toxins. Frontiers in Microbiology 11:959. [PubMed:32528435] experimental

(2) Julia McGillick et al. (2021) A YoeB toxin cleaves both RNA and DNA. Scientific Reports 11(1):3592. [PubMed:33574407] experimental


experimental experimental literature