6281

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/(antitoxin)
Location 1914763..1915282 Replicon chromosome
Accession NC_012924
Organism Streptococcus suis SC84

Toxin (Protein)


Gene name yoeB Uniprot ID A0A2K1SXY6
Locus tag SSUSC84_RS09420 Protein ID WP_012775363.1
Coordinates 1914763..1915020 (-) Length 86 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A0K2E7Z9
Locus tag SSUSC84_RS09425 Protein ID WP_002939010.1
Coordinates 1915022..1915282 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SSUSC84_RS09405 (SSUSC84_1814) 1911232..1912962 - 1731 WP_012775361.1 membrane protein -
SSUSC84_RS09410 (SSUSC84_1815) 1913327..1914235 - 909 WP_012027884.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
SSUSC84_RS09415 (SSUSC84_1816) 1914219..1914749 - 531 WP_009910909.1 DUF1697 domain-containing protein -
SSUSC84_RS09420 (SSUSC84_1817) 1914763..1915020 - 258 WP_012775363.1 Txe/YoeB family addiction module toxin Toxin
SSUSC84_RS09425 (SSUSC84_1818) 1915022..1915282 - 261 WP_002939010.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
SSUSC84_RS09430 (SSUSC84_1819) 1915358..1915813 - 456 WP_012028534.1 8-oxo-dGTP diphosphatase -
SSUSC84_RS09435 (SSUSC84_1820) 1915820..1916149 - 330 WP_012775364.1 type II toxin-antitoxin system RelE/ParE family toxin -
SSUSC84_RS09440 (SSUSC84_1821) 1916139..1916426 - 288 WP_012028535.1 hypothetical protein -
SSUSC84_RS09445 (SSUSC84_1822) 1916524..1916892 - 369 WP_012775365.1 hypothetical protein -
SSUSC84_RS09450 (SSUSC84_1823) 1916885..1917454 - 570 WP_012775366.1 ribonuclease M5 -
SSUSC84_RS09455 (SSUSC84_1824) 1917438..1918220 - 783 WP_012028536.1 TatD family hydrolase -
SSUSC84_RS09460 (SSUSC84_1825) 1918327..1918464 - 138 WP_002939016.1 50S ribosomal protein L34 -
SSUSC84_RS09465 (SSUSC84_1826) 1918632..1919627 - 996 WP_012775367.1 protein jag -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1874993..1918220 43227


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(1-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-74)


Sequences

Toxin        


Download         Length: 86 a.a.        Molecular weight: 10346.81 Da        Isoelectric Point: 7.2737

>T6281 WP_012775363.1 NC_012924:c1915020-1914763 [Streptococcus suis SC84]
MGIHFTDDAWEDYLYWQGQDKKTLKRINKIIQDMQRHPFEGIAKPEPLKYDYQGAWSRRIDAENRLIYMVEADRLCILSL
KDHYR

Download         Length: 258 bp

>T6281 NC_012924:c1915020-1914763 [Streptococcus suis SC84]
ATGGGAATCCATTTTACAGACGACGCCTGGGAAGACTATCTTTATTGGCAGGGTCAGGATAAGAAAACACTGAAGAGAAT
CAATAAGATTATCCAGGATATGCAGCGACATCCTTTTGAAGGCATTGCCAAACCAGAACCTTTGAAGTATGATTATCAAG
GTGCCTGGTCTCGGAGAATTGATGCGGAGAATCGGTTGATTTATATGGTGGAAGCTGATCGGTTGTGCATCCTGTCGCTA
AAGGATCATTATAGGTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9980.36 Da        Isoelectric Point: 5.7134

>AT6281 WP_002939010.1 NC_012924:c1915282-1915022 [Streptococcus suis SC84]
MEAIVYSHFRNHLKDYMKKVNDEFEPLVVVNKNPEEDIVVLSKSEWDSLQETLAVARNTYLSQKVLRGMAKVKTGQTQER
NLIEAD

Download         Length: 261 bp

>AT6281 NC_012924:c1915282-1915022 [Streptococcus suis SC84]
ATGGAAGCTATTGTATATTCCCATTTTCGAAATCACTTAAAGGACTATATGAAAAAGGTCAATGACGAGTTTGAACCTTT
GGTCGTGGTTAATAAAAATCCAGAGGAAGATATTGTTGTACTCTCAAAGAGTGAGTGGGATAGTTTACAAGAAACCCTCG
CTGTTGCTCGGAACACTTATCTGTCTCAAAAAGTCCTACGTGGTATGGCTAAAGTCAAAACGGGACAAACCCAAGAACGA
AACCTGATAGAGGCGGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T728 Streptococcus pneumoniae R6

75

100

0.75
T4918 Actinobacillus pleuropneumoniae serovar 3 str. JL03

57.143

98.824

0.565
T6022 Enterococcus faecium

58.025

95.294

0.553
T6115 Enterococcus faecium

58.025

95.294

0.553
T10021 Lacticaseibacillus rhamnosus 51B

55.556

95.294

0.529
T6138 Lactobacillus rhamnosus Lc 705

55.556

95.294

0.529
T6143 Lactobacillus rhamnosus ATCC 8530

55.556

95.294

0.529
T6237 Lactobacillus rhamnosus Lc 705

55.556

95.294

0.529
T10020 Lacticaseibacillus rhamnosus strain 24

55.556

95.294

0.529
T6122 Streptomyces coelicolor A3(2)

51.22

97.619

0.5
T1043 Escherichia coli str. K-12 substr. MG1655

51.25

95.238

0.488
T310 Pseudomonas putida KT2440

48.81

98.824

0.482
T130 Mycobacterium tuberculosis H37Rv

48.78

96.471

0.471
T613 Staphylococcus aureus subsp. aureus MW2

44.706

100

0.447
T2427 Staphylococcus aureus subsp. aureus NCTC 8325

44.706

100

0.447
T10077 Agrobacterium tumefaciens str. C58

41.573

100

0.435
T1074 Staphylococcus aureus subsp. aureus N315

43.529

100

0.435
T10072 Edwardsiella tarda EIB202

36.145

97.647

0.353

Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value
AT728 Streptococcus pneumoniae R6

79.487

92.857

0.738
AT10182 Aggregatibacter actinomycetemcomitans D11S 1

57.143

100

0.571
AT6115 Enterococcus faecium

45.238

97.674

0.442
AT6022 Enterococcus faecium

45.238

97.674

0.442
AT6143 Lactobacillus rhamnosus ATCC 8530

40.964

96.512

0.395
AT6138 Lactobacillus rhamnosus Lc 705

40.964

96.512

0.395
AT6237 Lactobacillus rhamnosus Lc 705

40.964

96.512

0.395
AT10021 Lacticaseibacillus rhamnosus 51B

40.964

96.512

0.395
AT10020 Lacticaseibacillus rhamnosus strain 24

40.964

96.512

0.395
AT4918 Actinobacillus pleuropneumoniae serovar 3 str. JL03

33.333

100

0.337

Multiple sequence alignment    




Structures

Toxin

Source ID Structure
AlphaFold DB A0A2K1SXY6

Antitoxin

Source ID Structure
AlphaFold DB A0A0K2E7Z9

References

(1) Chengkun Zheng et al. (2015) Identification and characterization of the chromosomal yefM-yoeB toxin-antitoxin system of Streptococcus suis. Scientific Reports 5:13125. [PubMed:26272287] experimental


experimental experimental literature