10021

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/-
Location 190881..191403 Replicon chromosome
Accession NZ_JMSI01000003
Organism Lacticaseibacillus rhamnosus 51B

Toxin (Protein)


Gene name yoeB Uniprot ID C2JUK2
Locus tag LR51B_RS07265 Protein ID WP_005687756.1
Coordinates 190881..191141 (-) Length 87 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID C2JUK3
Locus tag LR51B_RS07260 Protein ID WP_005687754.1
Coordinates 191134..191403 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LR51B_RS07290 (LR51B_06505) 186297..186938 + 642 WP_005690763.1 3-keto-L-gulonate-6-phosphate decarboxylase UlaD -
LR51B_RS07285 (LR51B_06510) 187006..187785 + 780 WP_005690765.1 DeoR/GlpR family DNA-binding transcription regulator -
LR51B_RS07280 (LR51B_06515) 187970..188857 + 888 WP_014571690.1 L-ribulose-5-phosphate 3-epimerase -
LR51B_RS07275 (LR51B_06520) 188924..189745 - 822 WP_005690770.1 Cof-type HAD-IIB family hydrolase -
LR51B_RS07270 (LR51B_06525) 189942..190670 + 729 WP_014571689.1 L-ribulose-5-phosphate 4-epimerase -
LR51B_RS07265 (LR51B_06530) 190881..191141 - 261 WP_005687756.1 Txe/YoeB family addiction module toxin Toxin
LR51B_RS07260 (LR51B_06535) 191134..191403 - 270 WP_005687754.1 type II toxin-antitoxin system prevent-host-death family antitoxin Antitoxin
LR51B_RS07255 (LR51B_06540) 192029..192829 + 801 WP_005690776.1 SDR family oxidoreductase -
LR51B_RS07250 (LR51B_06545) 192856..194724 + 1869 WP_014571687.1 HTH domain-containing protein -
LR51B_RS07245 (LR51B_06550) 194725..195234 + 510 WP_005690780.1 transcriptional regulator GutM -
LR51B_RS07240 (LR51B_06555) 195247..195816 + 570 WP_005687747.1 PTS glucitol/sorbitol transporter subunit IIC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-85)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-74)


Sequences

Toxin        


Download         Length: 87 a.a.        Molecular weight: 10485.91 Da        Isoelectric Point: 9.6577

>T10021 WP_005687756.1 NZ_JMSI01000003:c191141-190881 [Lacticaseibacillus rhamnosus 51B]
MIKTWTDDAWADYMYWHDQNDKRTIKRINQLIQAIDRDPYKGIGKPEPLRYALTGKWSRRIDQENRIIYSIEKNHINIFA
CRTHYS

Download         Length: 261 bp

>T10021 NZ_JMSI01000003:c191141-190881 [Lacticaseibacillus rhamnosus 51B]
ATGATTAAAACCTGGACCGATGATGCTTGGGCGGACTACATGTATTGGCATGATCAAAACGACAAGCGGACAATCAAACG
AATTAATCAACTCATTCAAGCCATTGACCGTGACCCTTATAAAGGCATCGGAAAACCTGAGCCACTTAGATATGCGCTAA
CCGGAAAATGGTCACGTCGGATTGATCAGGAAAATCGCATCATCTACAGCATTGAAAAGAACCACATTAATATTTTCGCC
TGCCGCACTCACTACAGTTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10181.39 Da        Isoelectric Point: 4.6755

>AT10021 WP_005687754.1 NZ_JMSI01000003:c191403-191134 [Lacticaseibacillus rhamnosus 51B]
MEATNYSDFRRNLKHYMSQVNEDAEPLLVTAKDDDDNVVVMSKHDFDAIEETLYLLSNPKLMAKIKRGDAQIAAGKAKQH
ELLTDFDHD

Download         Length: 270 bp

>AT10021 NZ_JMSI01000003:c191403-191134 [Lacticaseibacillus rhamnosus 51B]
ATGGAAGCAACGAATTATAGTGATTTCCGCCGCAACCTTAAGCATTATATGAGTCAAGTCAACGAAGACGCCGAACCGCT
ACTGGTTACCGCTAAAGATGATGATGACAATGTGGTGGTTATGAGCAAGCACGATTTTGACGCCATCGAAGAAACCCTGT
ATTTACTCAGCAATCCCAAGCTGATGGCCAAAATCAAACGTGGTGATGCCCAAATTGCCGCTGGAAAGGCTAAACAGCAC
GAGTTGTTAACGGACTTCGATCATGATTAA

Similar Proteins

Only experimentally validated proteins are listed.


Structures

Toxin

Source ID Structure
AlphaFold DB A0A249N526

Antitoxin

Source ID Structure
AlphaFold DB A0A249N594

References

(1) K M Klimina et al. (2013) Identification and characterization of toxin-antitoxin systems in strains of Lactobacillus rhamnosus isolated from humans. Anaerobe 22:82-9. [PubMed:23727113] experimental

(2) Hans Krügel et al. (2015) Expression of the toxin-antitoxin genes yefM(Lrh), yoeB(Lrh) in human Lactobacillus rhamnosus isolates. Journal of Basic Microbiology 55(8):982-91. [PubMed:25832734] experimental


experimental experimental literature