10020

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/-
Location 15154..15676 Replicon chromosome
Accession NZ_JPZB01000034
Organism Lacticaseibacillus rhamnosus strain 24

Toxin (Protein)


Gene name yoeB Uniprot ID C2JUK2
Locus tag LR24_RS01695 Protein ID WP_005687756.1
Coordinates 15416..15676 (+) Length 87 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID C2JUK3
Locus tag LR24_RS01700 Protein ID WP_005687754.1
Coordinates 15154..15423 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LR24_RS01720 (LR24_12470) 10741..11310 - 570 WP_005687747.1 PTS glucitol/sorbitol transporter subunit IIC -
LR24_RS01715 (LR24_12475) 11323..11832 - 510 WP_005687749.1 transcriptional regulator GutM -
LR24_RS01710 (LR24_12480) 11833..13701 - 1869 WP_005687750.1 helix-turn-helix domain-containing protein -
LR24_RS01705 (LR24_12485) 13728..14528 - 801 WP_005687752.1 SDR family oxidoreductase -
LR24_RS01700 (LR24_12490) 15154..15423 + 270 WP_005687754.1 type II toxin-antitoxin system prevent-host-death family antitoxin Antitoxin
LR24_RS01695 (LR24_12495) 15416..15676 + 261 WP_005687756.1 Txe/YoeB family addiction module toxin Toxin
LR24_RS01690 (LR24_12500) 15887..16615 - 729 WP_005687757.1 L-ribulose-5-phosphate 4-epimerase -
LR24_RS01685 (LR24_12505) 16812..17633 + 822 WP_005687759.1 Cof-type HAD-IIB family hydrolase -
LR24_RS01680 (LR24_12510) 17700..18587 - 888 WP_005687761.1 L-ribulose-5-phosphate 3-epimerase -
LR24_RS01675 (LR24_12515) 18771..19550 - 780 WP_005690765.1 DeoR/GlpR family DNA-binding transcription regulator -
LR24_RS01670 (LR24_12520) 19618..20259 - 642 WP_005687764.1 3-keto-L-gulonate-6-phosphate decarboxylase UlaD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-85)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-74)


Sequences

Toxin        


Download         Length: 87 a.a.        Molecular weight: 10485.91 Da        Isoelectric Point: 9.6577

>T10020 WP_005687756.1 NZ_JPZB01000034:15416-15676 [Lacticaseibacillus rhamnosus]
MIKTWTDDAWADYMYWHDQNDKRTIKRINQLIQAIDRDPYKGIGKPEPLRYALTGKWSRRIDQENRIIYSIEKNHINIFA
CRTHYS

Download         Length: 261 bp

>T10020 NZ_JPZB01000034:15416-15676 [Lacticaseibacillus rhamnosus]
ATGATTAAAACCTGGACCGATGATGCTTGGGCGGACTACATGTATTGGCATGATCAAAACGACAAGCGGACAATCAAACG
AATTAATCAACTCATTCAAGCCATTGACCGTGACCCTTATAAAGGCATCGGAAAACCTGAGCCACTTAGATATGCGCTAA
CCGGAAAATGGTCACGTCGGATTGATCAGGAAAATCGCATCATCTACAGCATTGAAAAGAACCACATTAATATTTTCGCC
TGCCGCACTCACTACAGTTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10181.39 Da        Isoelectric Point: 4.6755

>AT10020 WP_005687754.1 NZ_JPZB01000034:15154-15423 [Lacticaseibacillus rhamnosus]
MEATNYSDFRRNLKHYMSQVNEDAEPLLVTAKDDDDNVVVMSKHDFDAIEETLYLLSNPKLMAKIKRGDAQIAAGKAKQH
ELLTDFDHD

Download         Length: 270 bp

>AT10020 NZ_JPZB01000034:15154-15423 [Lacticaseibacillus rhamnosus]
ATGGAAGCAACGAATTATAGTGATTTCCGCCGCAACCTTAAGCATTATATGAGTCAAGTCAACGAAGACGCCGAACCGCT
ACTGGTTACCGCTAAAGATGATGATGACAATGTGGTGGTTATGAGCAAGCACGATTTTGACGCCATCGAAGAAACCCTGT
ATTTACTCAGCAATCCCAAGCTGATGGCCAAAATCAAACGTGGTGATGCCCAAATTGCCGCTGGAAAGGCTAAACAGCAC
GAGTTGTTAACGGACTTCGATCATGATTAA

Similar Proteins

Only experimentally validated proteins are listed.


Structures

Toxin

Source ID Structure
AlphaFold DB A0A249N526

Antitoxin

Source ID Structure
AlphaFold DB A0A249N594

References

(1) K M Klimina et al. (2013) Identification and characterization of toxin-antitoxin systems in strains of Lactobacillus rhamnosus isolated from humans. Anaerobe 22:82-9. [PubMed:23727113] experimental

(2) Hans Krügel et al. (2015) Expression of the toxin-antitoxin genes yefM(Lrh), yoeB(Lrh) in human Lactobacillus rhamnosus isolates. Journal of Basic Microbiology 55(8):982-91. [PubMed:25832734] experimental


experimental experimental literature