2427

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/PHD-RelE
Location 2477181..2477698 Replicon chromosome
Accession NC_007795
Organism Staphylococcus aureus subsp. aureus NCTC 8325

Toxin (Protein)


Gene name yoeB Uniprot ID Q2G286
Locus tag SAOUHSC_02691 Protein ID 88196331
Coordinates 2477181..2477447 (-) Length 89 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID Q2G285
Locus tag SAOUHSC_02692 Protein ID 88196332
Coordinates 2477447..2477698 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SAOUHSC_02686 2472607..2473143 - 537 YP_501148.1 hypothetical protein -
SAOUHSC_02687 2473376..2474200 - 825 YP_501149.1 formate/nitrite transporter -
SAOUHSC_02688 2474417..2474599 - 183 YP_501150.1 hypothetical protein -
SAOUHSC_02689 2474683..2475150 - 468 YP_501151.1 hypothetical protein -
SAOUHSC_02690 2475336..2476886 - 1551 YP_501152.1 hypothetical protein -
SAOUHSC_02691 2477181..2477447 - 267 YP_501153.1 hypothetical protein Toxin
SAOUHSC_02692 2477447..2477698 - 252 YP_501154.1 hypothetical protein Antitoxin
SAOUHSC_02693 2477863..2477964 - 102 YP_501155.1 hypothetical protein -
SAOUHSC_02694 2477971..2478570 - 600 YP_501156.1 hypothetical protein -
SAOUHSC_02695 2478589..2478939 - 351 YP_501157.1 hypothetical protein -
SAOUHSC_02696 2479206..2480456 + 1251 YP_501158.1 methicillin resistance determinant protein FmhA -
SAOUHSC_02697 2480553..2481284 - 732 YP_501159.1 amino acid ABC transporter ATP-binding protein -
SAOUHSC_02698 2481281..2482000 - 720 YP_501160.1 amino acid ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-88)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-74)


Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10437.95 Da        Isoelectric Point: 9.9143

>T2427 88196331 NC_007795:c2477447-2477181 [Staphylococcus aureus subsp. aureus NCTC 8325]
MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Download         Length: 267 bp

>T2427 NC_007795:c2477447-2477181 [Staphylococcus aureus subsp. aureus NCTC 8325]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9433.60 Da        Isoelectric Point: 4.3407

>AT2427 88196332 NC_007795:c2477698-2477447 [Staphylococcus aureus subsp. aureus NCTC 8325]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Download         Length: 252 bp

>AT2427 NC_007795:c2477698-2477447 [Staphylococcus aureus subsp. aureus NCTC 8325]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T613 Staphylococcus aureus subsp. aureus MW2

100

100

1
T1074 Staphylococcus aureus subsp. aureus N315

98.864

100

0.989
T4918 Actinobacillus pleuropneumoniae serovar 3 str. JL03

55.294

98.837

0.547
T1043 Escherichia coli str. K-12 substr. MG1655

52.941

100

0.536
T6022 Enterococcus faecium

49.398

97.647

0.482
T6115 Enterococcus faecium

49.398

97.647

0.482
T6143 Lactobacillus rhamnosus ATCC 8530

48.193

96.512

0.465
T10020 Lacticaseibacillus rhamnosus strain 24

48.193

96.512

0.465
T6237 Lactobacillus rhamnosus Lc 705

48.193

96.512

0.465
T6138 Lactobacillus rhamnosus Lc 705

48.193

96.512

0.465
T10021 Lacticaseibacillus rhamnosus 51B

48.193

96.512

0.465
T6122 Streptomyces coelicolor A3(2)

44.706

100

0.452
T728 Streptococcus pneumoniae R6

45.783

98.81

0.452
T6281 Streptococcus suis SC84

44.706

100

0.447
T130 Mycobacterium tuberculosis H37Rv

41.667

98.824

0.412
T310 Pseudomonas putida KT2440

39.535

97.727

0.386
T10072 Edwardsiella tarda EIB202

38.202

100

0.386
T10077 Agrobacterium tumefaciens str. C58

37.079

100

0.375
T10182 Aggregatibacter actinomycetemcomitans D11S 1

34.568

97.59

0.337

Multiple sequence alignment    



Structures

Toxin

Source ID Structure
PDB 6L8F
PDB 6L8G
PDB 7CUA
PDB 6L8E

Antitoxin

Source ID Structure
AlphaFold DB Q2G285

References

(1) Lu Xue et al. (2020) Distinct oligomeric structures of the YoeB-YefM complex provide insights into the conditional cooperativity of type II toxin-antitoxin system. Nucleic Acids Research 48(18):10527-10541. [PubMed:32845304] 3D_structure


3D_structure protein structure literature