Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) Txe-Axe/-
Location 4963..5482 Replicon plasmid pS177
Accession NC_014959
Organism Enterococcus faecium

Toxin (Protein)


Gene name Txe Uniprot ID Q848U6
Locus tag HS770_RS00035 Protein ID WP_000588503.1
Coordinates 4963..5220 (-) Length 86 a.a.

Antitoxin (Protein)


Gene name Axe Uniprot ID Q83WJ8
Locus tag HS770_RS00040 Protein ID WP_000388479.1
Coordinates 5213..5482 (-) Length 90 a.a.

Genomic Context

HS770_RS00025HS770_RS00055HS770_RS00005HS770_RS00010HS770_RS00015HS770_RS00020HS770_RS00030HS770_RS00035HS770_RS00040HS770_RS00045HS770_RS00230HS770_RS00050HS770_RS00060HS770_RS00065
Location: 2721..3335 (615 bp)
Type: Others
Protein ID: WP_001261742.1
Location: 6565..7245 (681 bp)
Type: Others
Protein ID: WP_001015311.1
Location: 1..291 (291 bp)
Type: Others
Protein ID: WP_000026576.1
Location: 427..606 (180 bp)
Type: Others
Protein ID: WP_001021554.1
Location: 603..953 (351 bp)
Type: Others
Protein ID: WP_000568378.1
Location: 946..2271 (1326 bp)
Type: Others
Protein ID: WP_000969590.1
Location: 3500..4504 (1005 bp)
Type: Others
Protein ID: WP_000455809.1
Location: 4963..5220 (258 bp)
Type: Toxin
Protein ID: WP_000588503.1
Location: 5213..5482 (270 bp)
Type: Antitoxin
Protein ID: WP_000388479.1
Location: 5613..5792 (180 bp)
Type: Others
Protein ID: WP_001120991.1
Location: 5808..6008 (201 bp)
Type: Others
Protein ID: WP_001033544.1
Location: 6173..6493 (321 bp)
Type: Others
Protein ID: Protein_10
Location: 7536..9011 (1476 bp)
Type: Others
Protein ID: WP_000443455.1
Location: 8989..9942 (954 bp)
Type: Others
Protein ID: WP_000222571.1
ToxinAntitoxinOther genes
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Locus tag
Coordinates
Strand
Size (bp)
Protein ID
Product
Description
HS770_RS00005 (pS177_p01)1..291-291WP_000026576.1hypothetical protein-
HS770_RS00010427..606-180WP_001021554.1hypothetical protein-
HS770_RS00015 (pS177_p02)603..953-351WP_000568378.1hypothetical protein-
HS770_RS00020 (pS177_p03)946..2271-1326WP_000969590.1Y-family DNA polymerase-
HS770_RS00025 (pS177_p04)2721..3335+615WP_001261742.1recombinase family protein-
HS770_RS00030 (pS177_p05)3500..4504-1005WP_000455809.1hypothetical protein-
HS770_RS00035 (pS177_p06)4963..5220-258WP_000588503.1Txe/YoeB family addiction module toxinToxin
HS770_RS00040 (pS177_p07)5213..5482-270WP_000388479.1type II toxin-antitoxin system Phd/YefM family antitoxinAntitoxin
HS770_RS000455613..5792-180WP_001120991.1hypothetical protein-
HS770_RS002305808..6008-201WP_001033544.14-hydroxybenzoate octaprenyltransferase-
HS770_RS000506173..6493-321Protein_10hypothetical protein-
HS770_RS00055 (pS177_p10)6565..7245+681WP_001015311.1IS6-like element IS1216 family transposase-
HS770_RS00060 (pS177_p11)7536..9011-1476WP_000443455.1RNA-directed DNA polymerase-
HS770_RS00065 (pS177_p12)8989..9942-954WP_000222571.1IS30 family transposase-

Associated MGEs

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MGE
detail
Similar
MGEs
Relative
position
MGE Type
Cargo ARG
Virulence gene
Coordinates
Length (bp)
insideNon-Mobilizable plasmidVanHAX / aph(3')-III / ant(6)-Ia / erm(B)-1..3903239032


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-85)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-72)


Sequences

Toxin        


Download         Length: 86 a.a.        Molecular weight: 10206.53 Da        Isoelectric Point: 8.4369

>T6115 WP_000588503.1 NC_014959:c5220-4963 [Enterococcus faecium]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY

Download         Length: 258 bp

>T6115 NC_014959:c5220-4963 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10373.51 Da        Isoelectric Point: 4.4346

>AT6115 WP_000388479.1 NC_014959:c5482-5213 [Enterococcus faecium]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD

Download         Length: 270 bp

>AT6115 NC_014959:c5482-5213 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

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Protein
Organism
Identities (%)
Coverage (%)
Ha-value
T6022Enterococcus faecium

100

100

1

T1043Escherichia coli str. K-12 substr. MG1655

60.494

96.429

0.583

T10020Lacticaseibacillus rhamnosus strain 24

57.647

100

0.576

T10021Lacticaseibacillus rhamnosus 51B

57.647

100

0.576

T4918Actinobacillus pleuropneumoniae serovar 3 str. JL03

57.647

100

0.576

T6138Lactobacillus rhamnosus Lc 705

57.647

100

0.576

T6143Lactobacillus rhamnosus ATCC 8530

57.647

100

0.576

T6237Lactobacillus rhamnosus Lc 705

57.647

100

0.576

T728Streptococcus pneumoniae R6

55.294

100

0.56

T6281Streptococcus suis SC84

58.025

95.294

0.553
Showing 1 to 10 of 18 rows
1025
 rows per page

Multiple sequence alignment    


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Protein
Organism
Identities (%)
Coverage (%)
Ha-value
AT6022Enterococcus faecium

100

100

1

AT10182Aggregatibacter actinomycetemcomitans D11S 1

56.471

100

0.571

AT10021Lacticaseibacillus rhamnosus 51B

57.831

93.258

0.539

AT6138Lactobacillus rhamnosus Lc 705

57.831

93.258

0.539

AT6143Lactobacillus rhamnosus ATCC 8530

57.831

93.258

0.539

AT10020Lacticaseibacillus rhamnosus strain 24

57.831

93.258

0.539

AT6237Lactobacillus rhamnosus Lc 705

57.831

93.258

0.539

AT6281Streptococcus suis SC84

45.238

97.674

0.442

AT728Streptococcus pneumoniae R6

46.25

95.238

0.44

AT613Staphylococcus aureus subsp. aureus MW2

37.662

92.771

0.349
Showing 1 to 10 of 14 rows
1025
 rows per page

Multiple sequence alignment    




Structures

Toxin

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Source
ID
Structure
AlphaFold DBA0A828ZXQ8

Antitoxin

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Source
ID
Structure
AlphaFold DBA0A828ZWU7

References

(1) Elizabeth M Halvorsen et al. (2011) Txe, an endoribonuclease of the enterococcal Axe-Txe toxin-antitoxin system, cleaves mRNA and inhibits protein synthesis. Microbiology (Reading, England) 157(Pt 2):387-397. [PubMed:21030436] experimental


experimental experimental literature