Detailed information of TA system
Experimentally validatedOverview
TA module
Type | II | Classification (family/domain) | Txe-Axe/- |
Location | 4963..5482 | Replicon | plasmid pS177 |
Accession | NC_014959 | ||
Organism | Enterococcus faecium |
Toxin (Protein)
Gene name | Txe | Uniprot ID | Q848U6 |
Locus tag | HS770_RS00035 | Protein ID | WP_000588503.1 |
Coordinates | 4963..5220 (-) | Length | 86 a.a. |
Antitoxin (Protein)
Gene name | Axe | Uniprot ID | Q83WJ8 |
Locus tag | HS770_RS00040 | Protein ID | WP_000388479.1 |
Coordinates | 5213..5482 (-) | Length | 90 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
HS770_RS00005 (pS177_p01) | 1..291 | - | 291 | WP_000026576.1 | hypothetical protein | - |
HS770_RS00010 | 427..606 | - | 180 | WP_001021554.1 | hypothetical protein | - |
HS770_RS00015 (pS177_p02) | 603..953 | - | 351 | WP_000568378.1 | hypothetical protein | - |
HS770_RS00020 (pS177_p03) | 946..2271 | - | 1326 | WP_000969590.1 | Y-family DNA polymerase | - |
HS770_RS00025 (pS177_p04) | 2721..3335 | + | 615 | WP_001261742.1 | recombinase family protein | - |
HS770_RS00030 (pS177_p05) | 3500..4504 | - | 1005 | WP_000455809.1 | hypothetical protein | - |
HS770_RS00035 (pS177_p06) | 4963..5220 | - | 258 | WP_000588503.1 | Txe/YoeB family addiction module toxin | Toxin |
HS770_RS00040 (pS177_p07) | 5213..5482 | - | 270 | WP_000388479.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | Antitoxin |
HS770_RS00045 | 5613..5792 | - | 180 | WP_001120991.1 | hypothetical protein | - |
HS770_RS00230 | 5808..6008 | - | 201 | WP_001033544.1 | 4-hydroxybenzoate octaprenyltransferase | - |
HS770_RS00050 | 6173..6493 | - | 321 | Protein_10 | hypothetical protein | - |
HS770_RS00055 (pS177_p10) | 6565..7245 | + | 681 | WP_001015311.1 | IS6-like element IS1216 family transposase | - |
HS770_RS00060 (pS177_p11) | 7536..9011 | - | 1476 | WP_000443455.1 | RNA-directed DNA polymerase | - |
HS770_RS00065 (pS177_p12) | 8989..9942 | - | 954 | WP_000222571.1 | IS30 family transposase | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|---|---|---|---|---|---|---|
inside | Non-Mobilizable plasmid | VanHAX / aph(3')-III / ant(6)-Ia / erm(B) | - | 1..39032 | 39032 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 86 a.a. Molecular weight: 10206.53 Da Isoelectric Point: 8.4369
>T6115 WP_000588503.1 NC_014959:c5220-4963 [Enterococcus faecium]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY
Download Length: 258 bp
>T6115 NC_014959:c5220-4963 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA
Antitoxin
Download Length: 90 a.a. Molecular weight: 10373.51 Da Isoelectric Point: 4.4346
>AT6115 WP_000388479.1 NC_014959:c5482-5213 [Enterococcus faecium]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD
Download Length: 270 bp
>AT6115 NC_014959:c5482-5213 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA
Similar Proteins
Only experimentally validated proteins are listed.
Multiple sequence alignment
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|---|---|---|---|
AT6022 | Enterococcus faecium |
100 |
100 |
1 |
AT10182 | Aggregatibacter actinomycetemcomitans D11S 1 |
56.471 |
100 |
0.571 |
AT10021 | Lacticaseibacillus rhamnosus 51B |
57.831 |
93.258 |
0.539 |
AT6138 | Lactobacillus rhamnosus Lc 705 |
57.831 |
93.258 |
0.539 |
AT6143 | Lactobacillus rhamnosus ATCC 8530 |
57.831 |
93.258 |
0.539 |
AT10020 | Lacticaseibacillus rhamnosus strain 24 |
57.831 |
93.258 |
0.539 |
AT6237 | Lactobacillus rhamnosus Lc 705 |
57.831 |
93.258 |
0.539 |
AT6281 | Streptococcus suis SC84 |
45.238 |
97.674 |
0.442 |
AT728 | Streptococcus pneumoniae R6 |
46.25 |
95.238 |
0.44 |
AT613 | Staphylococcus aureus subsp. aureus MW2 |
37.662 |
92.771 |
0.349 |
AT1074 | Staphylococcus aureus subsp. aureus N315 |
37.662 |
92.771 |
0.349 |
AT2427 | Staphylococcus aureus subsp. aureus NCTC 8325 |
37.662 |
92.771 |
0.349 |
AT10077 | Agrobacterium tumefaciens str. C58 |
37.333 |
89.286 |
0.333 |
AT130 | Mycobacterium tuberculosis H37Rv |
34.177 |
88.764 |
0.303 |
Multiple sequence alignment
Structures
Toxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A828ZXQ8 |
Antitoxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A828ZWU7 |
References
(1) Elizabeth M Halvorsen et al. (2011) Txe, an endoribonuclease of the enterococcal Axe-Txe toxin-antitoxin system, cleaves mRNA and inhibits protein synthesis. Microbiology (Reading, England) 157(Pt 2):387-397. [PubMed:21030436]
experimental literature