Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Txe-Axe/Txe-RelB
Location 38605..39124 Replicon plasmid p2_ISMMS_VRE12
Accession NZ_CP018825
Organism Enterococcus faecium strain ISMMS_VRE_12

Toxin (Protein)


Gene name Txe Uniprot ID Q848U6
Locus tag AL026_RS00240 Protein ID WP_000588503.1
Coordinates 38867..39124 (+) Length 86 a.a.

Antitoxin (Protein)


Gene name Axe Uniprot ID Q83WJ8
Locus tag AL026_RS00235 Protein ID WP_000388479.1
Coordinates 38605..38874 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AL026_RS00205 34581..35261 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -
AL026_RS00210 35379..36059 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -
AL026_RS00215 36592..37272 - 681 WP_002287920.1 IS6-like element IS1216 family transposase -
AL026_RS00220 37348..37914 + 567 Protein_42 hypothetical protein -
AL026_RS00230 38295..38474 + 180 WP_001120991.1 hypothetical protein -
AL026_RS00235 38605..38874 + 270 WP_000388479.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
AL026_RS00240 38867..39124 + 258 WP_000588503.1 Txe/YoeB family addiction module toxin Toxin
AL026_RS00245 39583..40587 + 1005 WP_000455809.1 hypothetical protein -
AL026_RS00250 40752..41366 - 615 WP_001261742.1 recombinase family protein -
AL026_RS00255 41816..43141 + 1326 WP_000969590.1 Y-family DNA polymerase -
AL026_RS00260 43134..43484 + 351 WP_000568378.1 hypothetical protein -
AL026_RS00265 43481..43660 + 180 WP_001021554.1 hypothetical protein -
AL026_RS00270 43796..44086 + 291 WP_000026576.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Non-Mobilizable plasmid erm(B) / ant(6)-Ia / aph(3')-III / VanHAX - 1..45240 45240


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-85)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-72)


Sequences

Toxin        


Download         Length: 86 a.a.        Molecular weight: 10206.53 Da        Isoelectric Point: 8.4369

>T70640 WP_000588503.1 NZ_CP018825:38867-39124 [Enterococcus faecium]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY

Download         Length: 258 bp

>T70640 NZ_CP018825:38867-39124 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10373.51 Da        Isoelectric Point: 4.4346

>AT70640 WP_000388479.1 NZ_CP018825:38605-38874 [Enterococcus faecium]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD

Download         Length: 270 bp

>AT70640 NZ_CP018825:38605-38874 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A828ZXQ8

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZWU7

References