Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Txe-Axe/Txe-RelB
Location 32298..32817 Replicon plasmid pVB3895_p2
Accession NZ_CP072569
Organism Enterococcus faecium strain VB3895

Toxin (Protein)


Gene name Txe Uniprot ID Q848U6
Locus tag J7291_RS14705 Protein ID WP_000588503.1
Coordinates 32560..32817 (+) Length 86 a.a.

Antitoxin (Protein)


Gene name Axe Uniprot ID Q83WJ8
Locus tag J7291_RS14700 Protein ID WP_000388479.1
Coordinates 32298..32567 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J7291_RS14665 (J7291_14670) 27869..28285 - 417 WP_001232859.1 hypothetical protein -
J7291_RS14670 (J7291_14675) 28288..28572 - 285 WP_000353844.1 hypothetical protein -
J7291_RS14675 (J7291_14680) 28938..29162 + 225 WP_000410576.1 hypothetical protein -
J7291_RS14685 (J7291_14690) 30285..30965 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -
J7291_RS14690 (J7291_14695) 31041..31607 + 567 Protein_36 hypothetical protein -
J7291_RS15370 31772..31972 + 201 WP_001033544.1 4-hydroxybenzoate octaprenyltransferase -
J7291_RS14695 (J7291_14700) 31988..32167 + 180 WP_001120991.1 hypothetical protein -
J7291_RS14700 (J7291_14705) 32298..32567 + 270 WP_000388479.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
J7291_RS14705 (J7291_14710) 32560..32817 + 258 WP_000588503.1 Txe/YoeB family addiction module toxin Toxin
J7291_RS14710 (J7291_14715) 33455..34063 + 609 WP_224752223.1 hypothetical protein -
J7291_RS14715 (J7291_14720) 34197..35516 + 1320 WP_000202380.1 IS1380-like element IS1678 family transposase -
J7291_RS14720 (J7291_14725) 35729..35962 + 234 WP_002296232.1 hypothetical protein -
J7291_RS14725 (J7291_14730) 36127..36741 - 615 WP_001261742.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Non-Mobilizable plasmid erm(B) / ant(6)-Ia / aph(3')-III / VanHAX - 1..41278 41278
- inside IScluster/Tn erm(B) / ant(6)-Ia / aph(3')-III / VanHAX - 1657..35516 33859


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-85)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-72)


Sequences

Toxin        


Download         Length: 86 a.a.        Molecular weight: 10206.53 Da        Isoelectric Point: 8.4369

>T198121 WP_000588503.1 NZ_CP072569:32560-32817 [Enterococcus faecium]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY

Download         Length: 258 bp

>T198121 NZ_CP096841:2773912-2774337 [Mycobacterium tuberculosis variant bovis]
GTGGCGCTGCTCGACGTCAACGCATTGGTCGCGCTGGCGTGGGACTCACACATCCACCACGCCCGGATCCGCGAGTGGTT
TACCGCCAACGCCACGCTCGGCTGGGCGACTTGCCCGCTCACCGAAGCCGGCTTCGTGCGGGTGTCGACGAACCCAAAAG
TACTTCCCAGCGCGATCGGGATCGCAGACGCTCGACGGGTCCTCGTGGCACTACGCGCCGTGGGAGGCCACCGCTTCCTG
GCTGACGACGTATCGCTCGTCGATGACGATGTTCCGTTGATCGTCGGTTATCGCCAGGTGACCGACGCCCATCTGCTGAC
ACTCGCCCGCCGGCGCGGCGTCCGCCTGGTCACCTTCGACGCCGGTGTCTTCACCCTCGCCCAACAACGCCCCAAGACGC
CAGTGGAGCTGCTGACCATCCTCTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10373.51 Da        Isoelectric Point: 4.4346

>AT198121 WP_000388479.1 NZ_CP072569:32298-32567 [Enterococcus faecium]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD

Download         Length: 270 bp

>AT198121 NZ_CP096841:2773685-2773906 [Mycobacterium tuberculosis variant bovis]
GTGCGAACGACACTTGACCTTGACGACGATGTGATCGCCGCGGCACGTGAACTTGCCTCCAGCCAGCGCCGCTCGCTCGG
CTCGGTGATTTCCGAACTCGCACGCCGTGGTCTCATGCCCGGACGCGTCGAGGCTGACGACGGGCTGCCGGTGATCCGCG
TTCCAGCCGGGACCCCGCCGATCACACCGGAGATGGTCCGTCGCGCGCTCGATGAGGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A828ZXQ8

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZWU7

References