Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Txe-Axe/Txe-RelB
Location 36220..36739 Replicon plasmid unnamed_4
Accession NZ_CP064428
Organism Enterococcus faecium strain PR01996-12

Toxin (Protein)


Gene name Txe Uniprot ID Q848U6
Locus tag IUH71_RS15435 Protein ID WP_000588503.1
Coordinates 36482..36739 (+) Length 86 a.a.

Antitoxin (Protein)


Gene name Axe Uniprot ID Q83WJ8
Locus tag IUH71_RS15430 Protein ID WP_000388479.1
Coordinates 36220..36489 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IUH71_RS15400 (IUH71_15400) 31288..32241 + 954 WP_002287760.1 IS30 family transposase -
IUH71_RS15405 (IUH71_15405) 32219..33694 + 1476 WP_000443455.1 RNA-directed DNA polymerase -
IUH71_RS15410 (IUH71_15410) 34032..34172 + 141 Protein_37 type I restriction-modification system subunit M N-terminal domain-containing protein -
IUH71_RS15415 (IUH71_15415) 34231..34911 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -
IUH71_RS15420 (IUH71_15420) 35029..35709 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -
IUH71_RS15425 (IUH71_15425) 35910..36089 + 180 WP_001120991.1 hypothetical protein -
IUH71_RS15430 (IUH71_15430) 36220..36489 + 270 WP_000388479.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
IUH71_RS15435 (IUH71_15435) 36482..36739 + 258 WP_000588503.1 Txe/YoeB family addiction module toxin Toxin
IUH71_RS15440 (IUH71_15440) 37198..38202 + 1005 WP_000455809.1 hypothetical protein -
IUH71_RS15445 (IUH71_15445) 38367..38981 - 615 WP_001261742.1 recombinase family protein -
IUH71_RS15450 (IUH71_15450) 39431..40756 + 1326 WP_000969590.1 Y-family DNA polymerase -
IUH71_RS15455 (IUH71_15455) 40749..41099 + 351 WP_000568378.1 hypothetical protein -
IUH71_RS15460 (IUH71_15460) 41096..41275 + 180 WP_001021554.1 hypothetical protein -
IUH71_RS15465 (IUH71_15465) 41411..41701 + 291 WP_000026576.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Non-Mobilizable plasmid erm(B) / ant(6)-Ia / aph(3')-III / VanHAX - 1..43518 43518


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-85)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-72)


Sequences

Toxin        


Download         Length: 86 a.a.        Molecular weight: 10206.53 Da        Isoelectric Point: 8.4369

>T181269 WP_000588503.1 NZ_CP064428:36482-36739 [Enterococcus faecium]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY

Download         Length: 258 bp

>T181269 NZ_CP085816:c3599728-3599420 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCAATACATGGTTTATCGCAATAAAGGAAACAGCAAGGCTTATCCTTATTTACTTGATGTGCAAAGCGATATTATTGA
TGAACTGCATACTCGCATGGTTATCCCATTGTTTCCTGTGAGCAGATTGGTAAATAACCCAGTTAAGCGCCTCACGCCCA
CTCTGAATGTGGAAGGTAATGACTATCTGGTGATGACTCACGAAATGGCGAGTATCAGGCTTTCGCAGATTGGTGATGAG
GTGATGGACGTTCGTTCCCACAGGCAAACCATCAAAAATGCTCTCGATTTTATCTTTGACGGGTTCTGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10373.51 Da        Isoelectric Point: 4.4346

>AT181269 WP_000388479.1 NZ_CP064428:36220-36489 [Enterococcus faecium]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD

Download         Length: 270 bp

>AT181269 NZ_CP085816:c3599970-3599731 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCCGGGTATGAGTGTACGACATAAAAAAACAGTCAGTGTCACGCTGGAACCTGCATTGCTTCAGCAAGCCCGTGATGC
AGGTATTAATCTGTCAGCGGTGCTTACCGCCGCGCTGAAAGAGGAAATCAGTGCCACAGGCGTTGAACGCTGGAAAGCAG
AAAATAGAGCGGGTTTGCAGGAGCTCAATCGTATTACTGATGAGCACGGCCTTTTATCGGACGATTACAGGACGTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A828ZXQ8

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZWU7

References