70647

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Txe-Axe/Txe-RelB
Location 40112..40631 Replicon plasmid p2_ISMMS_VRE9
Accession NZ_CP018831
Organism Enterococcus faecium strain ISMMS_VRE_9

Toxin (Protein)


Gene name Txe Uniprot ID Q848U6
Locus tag AL023_RS15050 Protein ID WP_000588503.1
Coordinates 40374..40631 (+) Length 86 a.a.

Antitoxin (Protein)


Gene name Axe Uniprot ID Q83WJ8
Locus tag AL023_RS15045 Protein ID WP_000388479.1
Coordinates 40112..40381 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AL023_RS15015 36088..36768 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -
AL023_RS15020 36886..37566 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -
AL023_RS15025 38099..38779 - 681 WP_002287920.1 IS6-like element IS1216 family transposase -
AL023_RS15030 38855..39421 + 567 Protein_44 hypothetical protein -
AL023_RS15040 39802..39981 + 180 WP_001120991.1 hypothetical protein -
AL023_RS15045 40112..40381 + 270 WP_000388479.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
AL023_RS15050 40374..40631 + 258 WP_000588503.1 Txe/YoeB family addiction module toxin Toxin
AL023_RS15055 41090..42094 + 1005 WP_000455809.1 hypothetical protein -
AL023_RS15060 42259..42873 - 615 WP_001261742.1 recombinase family protein -
AL023_RS15065 43323..44648 + 1326 WP_000969590.1 Y-family DNA polymerase -
AL023_RS15070 44641..44991 + 351 WP_000568378.1 hypothetical protein -
AL023_RS15075 44988..45167 + 180 WP_001021554.1 hypothetical protein -
AL023_RS15080 45303..45593 + 291 WP_000026576.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Non-Mobilizable plasmid erm(B) / ant(6)-Ia / aph(3')-III / VanHAX - 1..46747 46747


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-85)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-72)


Sequences

Toxin        


Download         Length: 86 a.a.        Molecular weight: 10206.53 Da        Isoelectric Point: 8.4369

>T70647 WP_000588503.1 NZ_CP018831:40374-40631 [Enterococcus faecium]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY

Download         Length: 258 bp

>T70647 NZ_CP018831:40374-40631 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10373.51 Da        Isoelectric Point: 4.4346

>AT70647 WP_000388479.1 NZ_CP018831:40112-40381 [Enterococcus faecium]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD

Download         Length: 270 bp

>AT70647 NZ_CP018831:40112-40381 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A828ZXQ8

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZWU7

References