Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/RelE(antitoxin)
Location 2755711..2756233 Replicon chromosome
Accession NC_017491
Organism Lactobacillus rhamnosus ATCC 8530

Toxin (Protein)


Gene name yoeB Uniprot ID C2JUK2
Locus tag LRHK_RS12800 Protein ID WP_005687756.1
Coordinates 2755973..2756233 (+) Length 87 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID C2JUK3
Locus tag LRHK_RS12795 Protein ID WP_005687754.1
Coordinates 2755711..2755980 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRHK_RS12775 (LRHK_2831) 2751298..2751867 - 570 WP_005687747.1 PTS glucitol/sorbitol transporter subunit IIC -
LRHK_RS12780 (LRHK_2832) 2751880..2752389 - 510 WP_005690780.1 transcriptional regulator GutM -
LRHK_RS12785 (LRHK_2833) 2752390..2754258 - 1869 WP_014571687.1 HTH domain-containing protein -
LRHK_RS12790 (LRHK_2834) 2754285..2755085 - 801 WP_005690776.1 SDR family oxidoreductase -
LRHK_RS12795 (LRHK_2836) 2755711..2755980 + 270 WP_005687754.1 type II toxin-antitoxin system prevent-host-death family antitoxin Antitoxin
LRHK_RS12800 (LRHK_2837) 2755973..2756233 + 261 WP_005687756.1 Txe/YoeB family addiction module toxin Toxin
LRHK_RS12805 (LRHK_2838) 2756444..2757172 - 729 WP_014571689.1 L-ribulose-5-phosphate 4-epimerase -
LRHK_RS12810 (LRHK_2840) 2757369..2758190 + 822 WP_005690770.1 Cof-type HAD-IIB family hydrolase -
LRHK_RS12815 (LRHK_2841) 2758257..2759144 - 888 WP_014571690.1 L-ribulose-5-phosphate 3-epimerase -
LRHK_RS12820 (LRHK_2842) 2759329..2760108 - 780 WP_005690765.1 DeoR/GlpR family DNA-binding transcription regulator -
LRHK_RS12825 (LRHK_2843) 2760176..2760817 - 642 WP_005690763.1 3-keto-L-gulonate-6-phosphate decarboxylase UlaD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-85)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-74)


Sequences

Toxin        


Download         Length: 87 a.a.        Molecular weight: 10485.91 Da        Isoelectric Point: 9.6577

>T6143 WP_005687756.1 NC_017491:2755973-2756233 [Lactobacillus rhamnosus ATCC 8530]
MIKTWTDDAWADYMYWHDQNDKRTIKRINQLIQAIDRDPYKGIGKPEPLRYALTGKWSRRIDQENRIIYSIEKNHINIFA
CRTHYS

Download         Length: 261 bp

>T6143 NC_017491:2755973-2756233 [Lactobacillus rhamnosus ATCC 8530]
ATGATTAAAACCTGGACCGATGATGCTTGGGCGGACTACATGTATTGGCATGATCAAAACGACAAGCGGACAATCAAACG
AATTAATCAACTCATTCAAGCCATTGACCGTGACCCTTATAAAGGCATCGGAAAACCTGAGCCACTTAGATATGCGCTAA
CCGGAAAATGGTCACGTCGGATTGATCAGGAAAATCGCATCATCTACAGCATTGAAAAGAACCACATTAATATTTTCGCC
TGCCGCACTCACTACAGTTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10181.39 Da        Isoelectric Point: 4.6755

>AT6143 WP_005687754.1 NC_017491:2755711-2755980 [Lactobacillus rhamnosus ATCC 8530]
MEATNYSDFRRNLKHYMSQVNEDAEPLLVTAKDDDDNVVVMSKHDFDAIEETLYLLSNPKLMAKIKRGDAQIAAGKAKQH
ELLTDFDHD

Download         Length: 270 bp

>AT6143 NC_017491:2755711-2755980 [Lactobacillus rhamnosus ATCC 8530]
ATGGAAGCAACGAATTATAGTGATTTCCGCCGCAACCTTAAGCATTATATGAGTCAAGTCAACGAAGACGCCGAACCGCT
ACTGGTTACCGCTAAAGATGATGATGACAATGTGGTGGTTATGAGCAAGCACGATTTTGACGCCATCGAAGAAACCCTGT
ATTTACTCAGCAATCCCAAGCTGATGGCCAAAATCAAACGTGGTGATGCCCAAATTGCCGCTGGAAAGGCTAAACAGCAC
GAGTTGTTAACGGACTTCGATCATGATTAA

Similar Proteins

Only experimentally validated proteins are listed.


Structures

Toxin

Source ID Structure
AlphaFold DB A0A249N526

Antitoxin

Source ID Structure
AlphaFold DB A0A249N594

References

(1) K M Klimina et al. (2013) Identification and characterization of toxin-antitoxin systems in strains of Lactobacillus rhamnosus isolated from humans. Anaerobe 22:82-9. [PubMed:23727113] experimental


experimental experimental literature