6138

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/-
Location 2763807..2764329 Replicon chromosome
Accession NC_013199
Organism Lactobacillus rhamnosus Lc 705

Toxin (Protein)


Gene name yoeB Uniprot ID C2JUK2
Locus tag LC705_RS12925 Protein ID WP_005687756.1
Coordinates 2764069..2764329 (+) Length 87 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID C2JUK3
Locus tag LC705_RS12920 Protein ID WP_005687754.1
Coordinates 2763807..2764076 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LC705_RS12900 (LC705_02722) 2759394..2759963 - 570 WP_005687747.1 PTS glucitol/sorbitol transporter subunit IIC -
LC705_RS12905 (LC705_02723) 2759976..2760485 - 510 WP_005690780.1 transcriptional regulator GutM -
LC705_RS12910 (LC705_02724) 2760486..2762354 - 1869 WP_014571687.1 HTH domain-containing protein -
LC705_RS12915 (LC705_02725) 2762381..2763181 - 801 WP_005690776.1 SDR family oxidoreductase -
LC705_RS12920 (LC705_02726) 2763807..2764076 + 270 WP_005687754.1 type II toxin-antitoxin system prevent-host-death family antitoxin Antitoxin
LC705_RS12925 (LC705_02727) 2764069..2764329 + 261 WP_005687756.1 Txe/YoeB family addiction module toxin Toxin
LC705_RS12930 (LC705_02728) 2764540..2765268 - 729 WP_014571689.1 L-ribulose-5-phosphate 4-epimerase -
LC705_RS12935 (LC705_02729) 2765465..2766286 + 822 WP_005690770.1 Cof-type HAD-IIB family hydrolase -
LC705_RS12940 (LC705_02730) 2766353..2767240 - 888 WP_014571690.1 L-ribulose-5-phosphate 3-epimerase -
LC705_RS12945 (LC705_02731) 2767425..2768204 - 780 WP_173011709.1 DeoR/GlpR family DNA-binding transcription regulator -
LC705_RS12950 (LC705_02732) 2768272..2768913 - 642 WP_005690763.1 3-keto-L-gulonate-6-phosphate decarboxylase UlaD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-85)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-74)


Sequences

Toxin        


Download         Length: 87 a.a.        Molecular weight: 10485.91 Da        Isoelectric Point: 9.6577

>T6138 WP_005687756.1 NC_013199:2764069-2764329 [Lactobacillus rhamnosus Lc 705]
MIKTWTDDAWADYMYWHDQNDKRTIKRINQLIQAIDRDPYKGIGKPEPLRYALTGKWSRRIDQENRIIYSIEKNHINIFA
CRTHYS

Download         Length: 261 bp

>T6138 NC_013199:2764069-2764329 [Lactobacillus rhamnosus Lc 705]
ATGATTAAAACCTGGACCGATGATGCTTGGGCGGACTACATGTATTGGCATGATCAAAACGACAAGCGGACAATCAAACG
AATTAATCAACTCATTCAAGCCATTGACCGTGACCCTTATAAAGGCATCGGAAAACCTGAGCCACTTAGATATGCGCTAA
CCGGAAAATGGTCACGTCGGATTGATCAGGAAAATCGCATCATCTACAGCATTGAAAAGAACCACATTAATATTTTCGCC
TGCCGCACTCACTACAGTTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10181.39 Da        Isoelectric Point: 4.6755

>AT6138 WP_005687754.1 NC_013199:2763807-2764076 [Lactobacillus rhamnosus Lc 705]
MEATNYSDFRRNLKHYMSQVNEDAEPLLVTAKDDDDNVVVMSKHDFDAIEETLYLLSNPKLMAKIKRGDAQIAAGKAKQH
ELLTDFDHD

Download         Length: 270 bp

>AT6138 NC_013199:2763807-2764076 [Lactobacillus rhamnosus Lc 705]
ATGGAAGCAACGAATTATAGTGATTTCCGCCGCAACCTTAAGCATTATATGAGTCAAGTCAACGAAGACGCCGAACCGCT
ACTGGTTACCGCTAAAGATGATGATGACAATGTGGTGGTTATGAGCAAGCACGATTTTGACGCCATCGAAGAAACCCTGT
ATTTACTCAGCAATCCCAAGCTGATGGCCAAAATCAAACGTGGTGATGCCCAAATTGCCGCTGGAAAGGCTAAACAGCAC
GAGTTGTTAACGGACTTCGATCATGATTAA

Similar Proteins

Only experimentally validated proteins are listed.


Structures

Toxin

Source ID Structure
AlphaFold DB A0A249N526

Antitoxin

Source ID Structure
AlphaFold DB A0A249N594

References

(1) K M Klimina et al. (2013) Identification and characterization of toxin-antitoxin systems in strains of Lactobacillus rhamnosus isolated from humans. Anaerobe 22:82-9. [PubMed:23727113] experimental


experimental experimental literature