Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/PHD-RelE |
| Location | 2522494..2523017 | Replicon | chromosome |
| Accession | NC_002745 | ||
| Organism | Staphylococcus aureus subsp. aureus N315 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | A0A3A5LQI3 |
| Locus tag | SA_RS12895 | Protein ID | WP_000074049.1 |
| Coordinates | 2522494..2522760 (-) | Length | 89 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | W8TQT5 |
| Locus tag | SA_RS12900 | Protein ID | WP_000587616.1 |
| Coordinates | 2522760..2523017 (-) | Length | 86 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| SA_RS12875 (SA2241) | 2517801..2518988 | - | 1188 | WP_000026189.1 | MFS transporter | - |
| SA_RS12880 (SA2242) | 2519384..2520160 | - | 777 | WP_000072149.1 | iron export ABC transporter permease subunit FetB | - |
| SA_RS12885 (SA2243) | 2520153..2520815 | - | 663 | WP_000923526.1 | ATP-binding cassette domain-containing protein | - |
| SA_RS12890 (SA2244) | 2521063..2522139 | - | 1077 | WP_001076673.1 | M42 family metallopeptidase | - |
| SA_RS12895 (SA2245) | 2522494..2522760 | - | 267 | WP_000074049.1 | Txe/YoeB family addiction module toxin | Toxin |
| SA_RS12900 (SA2246) | 2522760..2523017 | - | 258 | WP_000587616.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | Antitoxin |
| SA_RS12910 (SA2247) | 2523376..2523831 | + | 456 | WP_000732076.1 | DUF1307 domain-containing protein | - |
| SA_RS12915 (SA2248) | 2523999..2525576 | - | 1578 | WP_000143494.1 | FMN-binding glutamate synthase family protein | - |
| SA_RS12920 (SA2249) | 2525771..2526520 | + | 750 | WP_000072461.1 | CPBP family intramembrane metalloprotease | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 89 a.a. Molecular weight: 10676.24 Da Isoelectric Point: 10.1597
>T1075 WP_000074049.1 NC_002745:c2522760-2522494 [Staphylococcus aureus subsp. aureus N315]
MSNYTVKIKNSAKSDLRKIKHSYLKKSFLEIVETLKNDPYKITQSFEKLEPKYLERYSRRINHQHRVVYTVDDRNKEVLI
LSAWSHYD
MSNYTVKIKNSAKSDLRKIKHSYLKKSFLEIVETLKNDPYKITQSFEKLEPKYLERYSRRINHQHRVVYTVDDRNKEVLI
LSAWSHYD
Download Length: 267 bp
>T1075 NC_002745:c2522760-2522494 [Staphylococcus aureus subsp. aureus N315]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAGGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAGGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA
Antitoxin
Download Length: 86 a.a. Molecular weight: 9774.73 Da Isoelectric Point: 4.1353
>AT1075 WP_000587616.1 NC_002745:c2523017-2522760 [Staphylococcus aureus subsp. aureus N315]
MIITSPTEARKDFYQLLKNVNNNHEPIYISGNNAENNAVIIGLEDWKSIQETIYLESTGTMDKVREREKDNSGTTNIDDI
DWDNL
MIITSPTEARKDFYQLLKNVNNNHEPIYISGNNAENNAVIIGLEDWKSIQETIYLESTGTMDKVREREKDNSGTTNIDDI
DWDNL
Download Length: 258 bp
>AT1075 NC_002745:c2523017-2522760 [Staphylococcus aureus subsp. aureus N315]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T614 | Staphylococcus aureus subsp. aureus MW2 |
98.864 |
100 |
0.989 |
| T10043 | Staphylococcus aureus subsp. aureus NCTC 8325 |
98.864 |
100 |
0.989 |
| T10072 | Edwardsiella tarda EIB202 |
29.032 |
100 |
0.307 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT614 | Staphylococcus aureus subsp. aureus MW2 |
100 |
100 |
1 |
| AT10043 | Staphylococcus aureus subsp. aureus NCTC 8325 |
100 |
100 |
1 |
Multiple sequence alignment
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3A5LQI3 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | W8TQT5 |
References
(1) Niles P Donegan et al. (2010) Proteolytic regulation of toxin-antitoxin systems by ClpPC in Staphylococcus aureus. Journal of Bacteriology 192(5):1416-22. [PubMed:20038589]