Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | yefM-yoeB (relBE)/- |
| Location | 2534034..2534557 | Replicon | chromosome |
| Accession | NC_007795 | ||
| Organism | Staphylococcus aureus subsp. aureus NCTC 8325 | ||
Toxin (Protein)
| Gene name | yoeB | Uniprot ID | Q2FVF8 |
| Locus tag | SAOUHSC_02756 | Protein ID | YP_501215.1 |
| Coordinates | 2534034..2534300 (-) | Length | 89 a.a. |
Antitoxin (Protein)
| Gene name | yefM | Uniprot ID | Q2FVF7 |
| Locus tag | SAOUHSC_02757 | Protein ID | YP_501216.1 |
| Coordinates | 2534300..2534557 (-) | Length | 86 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| SAOUHSC_02752 | 2529341..2530468 | - | 1128 | YP_501211.1 | hypothetical protein | - |
| SAOUHSC_02753 | 2530924..2531700 | - | 777 | YP_501212.1 | hypothetical protein | - |
| SAOUHSC_02754 | 2531693..2532355 | - | 663 | YP_501213.1 | ABC transporter ATP-binding protein | - |
| SAOUHSC_02755 | 2532603..2533679 | - | 1077 | YP_501214.1 | hypothetical protein | - |
| SAOUHSC_02756 | 2534034..2534300 | - | 267 | YP_501215.1 | hypothetical protein | Toxin |
| SAOUHSC_02757 | 2534300..2534557 | - | 258 | YP_501216.1 | hypothetical protein | Antitoxin |
| SAOUHSC_02759 | 2534916..2535371 | + | 456 | YP_501218.1 | hypothetical protein | - |
| SAOUHSC_02760 | 2535539..2537116 | - | 1578 | YP_501219.1 | glutamate synthase subunit alpha | - |
| SAOUHSC_02761 | 2537311..2538060 | + | 750 | YP_501220.1 | hypothetical protein | - |
| SAOUHSC_02762 | 2538284..2539477 | - | 1194 | YP_501221.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 89 a.a. Molecular weight: 10648.23 Da Isoelectric Point: 10.1384
>T10043 YP_501215.1 NC_007795:c2534300-2534034 [Staphylococcus aureus subsp. aureus NCTC 8325]
MSNYTVKIKNSAKSDLKKIKHSYLKKSFLEIVETLKNDPYKITQSFEKLEPKYLERYSRRINHQHRVVYTVDDRNKEVLI
LSAWSHYD
MSNYTVKIKNSAKSDLKKIKHSYLKKSFLEIVETLKNDPYKITQSFEKLEPKYLERYSRRINHQHRVVYTVDDRNKEVLI
LSAWSHYD
Download Length: 267 bp
>T10043 NC_007795:c2534300-2534034 [Staphylococcus aureus subsp. aureus NCTC 8325]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAAGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAAGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA
Antitoxin
Download Length: 86 a.a. Molecular weight: 9774.73 Da Isoelectric Point: 4.1353
>AT10043 YP_501216.1 NC_007795:c2534557-2534300 [Staphylococcus aureus subsp. aureus NCTC 8325]
MIITSPTEARKDFYQLLKNVNNNHEPIYISGNNAENNAVIIGLEDWKSIQETIYLESTGTMDKVREREKDNSGTTNIDDI
DWDNL
MIITSPTEARKDFYQLLKNVNNNHEPIYISGNNAENNAVIIGLEDWKSIQETIYLESTGTMDKVREREKDNSGTTNIDDI
DWDNL
Download Length: 258 bp
>AT10043 NC_007795:c2534557-2534300 [Staphylococcus aureus subsp. aureus NCTC 8325]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T614 | Staphylococcus aureus subsp. aureus MW2 |
100 |
100 |
1 |
| T1075 | Staphylococcus aureus subsp. aureus N315 |
98.864 |
100 |
0.989 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT614 | Staphylococcus aureus subsp. aureus MW2 |
100 |
100 |
1 |
| AT1075 | Staphylococcus aureus subsp. aureus N315 |
100 |
100 |
1 |
Multiple sequence alignment
References
(1) Lu Xue et al. (2022) The two paralogous copies of the YoeB-YefM toxin-antitoxin module in Staphylococcus aureus differ in DNA binding and recognition patterns. The Journal of Biological Chemistry 298(1):101457. [PubMed:34861238]