Detailed information of TA system TA000614

Experimentally validated

Overview

TA module

• Type: II
• Classification (family/domain): relBE/PHD-RelE
• Location: 2535956..2536479
• Replicon: chromosome
• Accession: NC_003923
• Organism: Staphylococcus aureus subsp. aureus MW2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: MW2380
• Protein ID: 21284109
• Coordinates: 2535956..2536222 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: MW2381
• Protein ID: 21284110
• Coordinates: 2536222..2536479 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW_RS12895 (MW2376)	2531262..2532449	-	1188	WP_000026194.1	MFS transporter	-
MW_RS12900 (MW2377)	2532845..2533621	-	777	WP_000072145.1	iron export ABC transporter permease subunit FetB	-
MW_RS12905 (MW2378)	2533614..2534276	-	663	WP_000923523.1	ATP-binding cassette domain-containing protein	-
MW_RS12910 (MW2379)	2534524..2535600	-	1077	WP_001076671.1	M42 family metallopeptidase	-
MW_RS12915 (MW2380)	2535956..2536222	-	267	WP_000074048.1	Txe/YoeB family addiction module toxin	Toxin
MW_RS12920 (MW2381)	2536222..2536479	-	258	WP_000587616.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MW_RS12930 (MW2382)	2536838..2537293	+	456	WP_000732082.1	DUF1307 domain-containing protein	-
MW_RS12935 (MW2383)	2537461..2539038	-	1578	WP_000143496.1	FMN-binding glutamate synthase family protein	-
MW_RS12940 (MW2384)	2539234..2539983	+	750	WP_000072445.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (25-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-68)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10648.23 Da
• Isoelectric Point: 10.1384

>T614 21284109 NC_003923:c2536222-2535956 [Staphylococcus aureus subsp. aureus MW2]
MSNYTVKIKNSAKSDLKKIKHSYLKKSFLEIVETLKNDPYKITQSFEKLEPKYLERYSRRINHQHRVVYTVDDRNKEVLI
LSAWSHYD

Nucleotide

• Length: 267 bp

>T614 NC_003923:c2536222-2535956 [Staphylococcus aureus subsp. aureus MW2]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAAGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9774.73 Da
• Isoelectric Point: 4.1353

>AT614 21284110 NC_003923:c2536479-2536222 [Staphylococcus aureus subsp. aureus MW2]
MIITSPTEARKDFYQLLKNVNNNHEPIYISGNNAENNAVIIGLEDWKSIQETIYLESTGTMDKVREREKDNSGTTNIDDI
DWDNL

Nucleotide

• Length: 258 bp

>AT614 NC_003923:c2536479-2536222 [Staphylococcus aureus subsp. aureus MW2]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Toxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
T10043	Staphylococcus aureus subsp. aureus NCTC 8325	100	100	1
T1075	Staphylococcus aureus subsp. aureus N315	98.864	100	0.989

Multiple sequence alignment

Antitoxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
AT10043	Staphylococcus aureus subsp. aureus NCTC 8325	100	100	1
AT1075	Staphylococcus aureus subsp. aureus N315	100	100	1

Multiple sequence alignment

References

(1) Gul Habib et al. (2018) Bioinformatics and Functional Assessment of Toxin-Antitoxin Systems in Staphylococcus aureus. Toxins 10(11):473. [PubMed:30441856]


 experimental literature