Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | parDE/RHH-RelE |
| Location | 143567..144072 | Replicon | chromosome |
| Accession | NC_002516 | ||
| Organism | Pseudomonas aeruginosa PAO1 | ||
Toxin (Protein)
| Gene name | parE | Uniprot ID | Q9I708 |
| Locus tag | PA0124 | Protein ID | 15595322 |
| Coordinates | 143567..143848 (-) | Length | 94 a.a. |
Antitoxin (Protein)
| Gene name | parD | Uniprot ID | Q9I707 |
| Locus tag | PA0125 | Protein ID | 15595323 |
| Coordinates | 143845..144072 (-) | Length | 76 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| PA0119 | 138818..140167 | + | 1350 | NP_248809.1 | C4-dicarboxylate transporter DctA | - |
| PA0120 | 140216..140902 | + | 687 | NP_248810.1 | transcriptional regulator | - |
| PA0121 | 141003..141737 | + | 735 | NP_248811.1 | hypothetical protein | - |
| PA0122 | 141917..142327 | + | 411 | NP_248812.1 | hypothetical protein | - |
| PA0123 | 142359..143267 | - | 909 | NP_248813.1 | transcriptional regulator | - |
| PA0124 | 143567..143848 | - | 282 | NP_248814.1 | hypothetical protein | Toxin |
| PA0125 | 143845..144072 | - | 228 | NP_248815.1 | hypothetical protein | Antitoxin |
| PA0126 | 144248..144868 | - | 621 | NP_248816.1 | hypothetical protein | - |
| PA0127 | 144969..145469 | + | 501 | NP_248817.1 | hypothetical protein | - |
| PA0128 | 145542..145883 | + | 342 | NP_248818.1 | hypothetical protein | - |
| PA0129 | 145965..147392 | - | 1428 | NP_248819.1 | gamma-aminobutyrate permease | - |
| PA0130 | 147561..149054 | - | 1494 | NP_248820.1 | 3-oxopropanoate dehydrogenase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 94 a.a. Molecular weight: 10462.19 Da Isoelectric Point: 10.0435
>T180 15595322 NC_002516:c143848-143567 [Pseudomonas aeruginosa PAO1]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP
Download Length: 282 bp
>T180 NC_002516:c143848-143567 [Pseudomonas aeruginosa PAO1]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGAGTCGAAATTACCCCCTGA
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGAGTCGAAATTACCCCCTGA
Antitoxin
Download Length: 76 a.a. Molecular weight: 8568.64 Da Isoelectric Point: 5.5740
>AT180 15595323 NC_002516:c144072-143845 [Pseudomonas aeruginosa PAO1]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHIEIEKRWGLQ
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHIEIEKRWGLQ
Download Length: 228 bp
>AT180 NC_002516:c144072-143845 [Pseudomonas aeruginosa PAO1]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGACCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACATCGAGATCGAGAAGCGCTGGGGGCTGCAATGA
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGACCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACATCGAGATCGAGAAGCGCTGGGGGCTGCAATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T6097 | Sinorhizobium meliloti 1021 |
33.721 |
92.473 |
0.312 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT6134 | Paracoccus aminophilus |
40.351 |
76 |
0.307 |
Multiple sequence alignment
References
(1) Yingjie Song et al. (2023) Comparative analysis of five type II TA systems identified in Pseudomonas aeruginosa reveals their contributions to persistence and intracellular survival. Frontiers in Cellular And Infection Microbiology 13:1127786. [PubMed:36844395]
(2) Inga Songailiene et al. (2020) HEPN-MNT Toxin-Antitoxin System: The HEPN Ribonuclease Is Neutralized by OligoAMPylation. Molecular Cell 80(6):955-970.e7. [PubMed:33290744]
(3) Meenakumari Muthuramalingam et al. (2019) The toxin from a ParDE toxin-antitoxin system found in Pseudomonas aeruginosa offers protection to cells challenged with anti-gyrase antibiotics. Molecular Microbiology 111(2):441-454. [PubMed:30427086]
(4) Kevin J Snead et al. (2022) ParD Antitoxin Hotspot Alters a Disorder-to-Order Transition upon Binding to Its Cognate ParE Toxin, Lessening Its Interaction Affinity and Increasing Its Protease Degradation Kinetics. Biochemistry 61(1):34-45. [PubMed:34914378]