Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) parDE/RHH-RelE
Location 143567..144072 Replicon chromosome
Accession NC_002516
Organism Pseudomonas aeruginosa PAO1

Toxin (Protein)


Gene name parE Uniprot ID Q9I708
Locus tag PA0124 Protein ID 15595322
Coordinates 143567..143848 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID Q9I707
Locus tag PA0125 Protein ID 15595323
Coordinates 143845..144072 (-) Length 76 a.a.

Genomic Context

PA0119PA0120PA0121PA0122PA0127PA0128PA0123PA0124PA0125PA0126PA0129PA0130
Location: 138818..140167 (1350 bp)
Type: Others
Protein ID: NP_248809.1
Location: 140216..140902 (687 bp)
Type: Others
Protein ID: NP_248810.1
Location: 141003..141737 (735 bp)
Type: Others
Protein ID: NP_248811.1
Location: 141917..142327 (411 bp)
Type: Others
Protein ID: NP_248812.1
Location: 144969..145469 (501 bp)
Type: Others
Protein ID: NP_248817.1
Location: 145542..145883 (342 bp)
Type: Others
Protein ID: NP_248818.1
Location: 142359..143267 (909 bp)
Type: Others
Protein ID: NP_248813.1
Location: 143567..143848 (282 bp)
Type: Toxin
Protein ID: NP_248814.1
Location: 143845..144072 (228 bp)
Type: Antitoxin
Protein ID: NP_248815.1
Location: 144248..144868 (621 bp)
Type: Others
Protein ID: NP_248816.1
Location: 145965..147392 (1428 bp)
Type: Others
Protein ID: NP_248819.1
Location: 147561..149054 (1494 bp)
Type: Others
Protein ID: NP_248820.1
ToxinAntitoxinOther genes
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Locus tag
Coordinates
Strand
Size (bp)
Protein ID
Product
Description
PA0119138818..140167+1350NP_248809.1C4-dicarboxylate transporter DctA-
PA0120140216..140902+687NP_248810.1transcriptional regulator-
PA0121141003..141737+735NP_248811.1hypothetical protein-
PA0122141917..142327+411NP_248812.1hypothetical protein-
PA0123142359..143267-909NP_248813.1transcriptional regulator-
PA0124143567..143848-282NP_248814.1hypothetical proteinToxin
PA0125143845..144072-228NP_248815.1hypothetical proteinAntitoxin
PA0126144248..144868-621NP_248816.1hypothetical protein-
PA0127144969..145469+501NP_248817.1hypothetical protein-
PA0128145542..145883+342NP_248818.1hypothetical protein-
PA0129145965..147392-1428NP_248819.1gamma-aminobutyrate permease-
PA0130147561..149054-1494NP_248820.13-oxopropanoate dehydrogenase-

Associated MGEs

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MGE
detail
Similar
MGEs
Relative
position
MGE Type
Cargo ARG
Virulence gene
Coordinates
Length (bp)
No matching records found


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-87)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-42)


Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10462.19 Da        Isoelectric Point: 10.0435

>T180 15595322 NC_002516:c143848-143567 [Pseudomonas aeruginosa PAO1]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Download         Length: 282 bp

>T180 NC_002516:c143848-143567 [Pseudomonas aeruginosa PAO1]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGAGTCGAAATTACCCCCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8568.64 Da        Isoelectric Point: 5.5740

>AT180 15595323 NC_002516:c144072-143845 [Pseudomonas aeruginosa PAO1]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHIEIEKRWGLQ

Download         Length: 228 bp

>AT180 NC_002516:c144072-143845 [Pseudomonas aeruginosa PAO1]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGACCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACATCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

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Protein
Organism
Identities (%)
Coverage (%)
Ha-value
T6097Sinorhizobium meliloti 1021

33.721

92.473

0.312
Showing 1 to 1 of 1 rows

Multiple sequence alignment    


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Protein
Organism
Identities (%)
Coverage (%)
Ha-value
AT6134Paracoccus aminophilus

40.351

76

0.307
Showing 1 to 1 of 1 rows

Multiple sequence alignment    




Structures

Toxin

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Source
ID
Structure
PDB6XRW

Antitoxin

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Source
ID
Structure
PDB6XRW
AlphaFold DBQ9I707

References

(1) Yingjie Song et al. (2023) Comparative analysis of five type II TA systems identified in Pseudomonas aeruginosa reveals their contributions to persistence and intracellular survival. Frontiers in Cellular And Infection Microbiology 13:1127786. [PubMed:36844395] experimental

(2) Inga Songailiene et al. (2020) HEPN-MNT Toxin-Antitoxin System: The HEPN Ribonuclease Is Neutralized by OligoAMPylation. Molecular Cell 80(6):955-970.e7. [PubMed:33290744] experimental

(3) Meenakumari Muthuramalingam et al. (2019) The toxin from a ParDE toxin-antitoxin system found in Pseudomonas aeruginosa offers protection to cells challenged with anti-gyrase antibiotics. Molecular Microbiology 111(2):441-454. [PubMed:30427086] experimental

(4) Kevin J Snead et al. (2022) ParD Antitoxin Hotspot Alters a Disorder-to-Order Transition upon Binding to Its Cognate ParE Toxin, Lessening Its Interaction Affinity and Increasing Its Protease Degradation Kinetics. Biochemistry 61(1):34-45. [PubMed:34914378] experimental


experimental experimental literature