Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 3083112..3083583 Replicon chromosome
Accession NZ_CP028650
Organism Escherichia coli strain 124

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag C9E53_RS16245 Protein ID WP_001303511.1
Coordinates 3083305..3083583 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag C9E53_RS16240 Protein ID WP_001302048.1
Coordinates 3083112..3083303 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C9E53_RS16200 3078319..3078903 - 585 WP_000206793.1 DUF551 domain-containing protein -
C9E53_RS16205 3078960..3079355 - 396 WP_001118159.1 DUF977 family protein -
C9E53_RS16210 3079371..3080140 - 770 Protein_3081 DUF1627 domain-containing protein -
C9E53_RS16215 3080166..3080906 - 741 WP_000788938.1 ATP-binding protein -
C9E53_RS16220 3080913..3081875 - 963 WP_000095669.1 helix-turn-helix domain-containing protein -
C9E53_RS16225 3081898..3082323 - 426 WP_000693943.1 Rha family transcriptional regulator -
C9E53_RS16230 3082320..3082622 - 303 WP_000172738.1 transcriptional regulator -
C9E53_RS16235 3082720..3083091 + 372 WP_001169686.1 hypothetical protein -
C9E53_RS16240 3083112..3083303 + 192 WP_001302048.1 hypothetical protein Antitoxin
C9E53_RS16245 3083305..3083583 + 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C9E53_RS16250 3083936..3084235 + 300 WP_001240334.1 hypothetical protein -
C9E53_RS16255 3084307..3084525 + 219 WP_001171930.1 hypothetical protein -
C9E53_RS16265 3085094..3085282 + 189 WP_000449192.1 cell division inhibition protein DicB -
C9E53_RS16270 3085279..3085470 + 192 WP_001090200.1 DUF1482 family protein -
C9E53_RS16275 3085563..3088034 + 2472 WP_000048458.1 exonuclease -
C9E53_RS16280 3088107..3088358 + 252 WP_000005552.1 excisionase family protein -
C9E53_RS16285 3088393..3088580 + 188 Protein_3095 DUF3596 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - nleG7' 2992409..3098463 106054


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T102269 WP_001303511.1 NZ_CP028650:3083305-3083583 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T102269 NZ_CP028650:3083305-3083583 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT102269 WP_001302048.1 NZ_CP028650:3083112-3083303 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT102269 NZ_CP028650:3083112-3083303 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References