Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2510047..2510518 Replicon chromosome
Accession NZ_CP017446
Organism Escherichia coli O157:H7 strain 9234

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag A4C47_RS12895 Protein ID WP_001303511.1
Coordinates 2510047..2510325 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag A4C47_RS12900 Protein ID WP_001302048.1
Coordinates 2510327..2510518 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A4C47_RS30910 2505053..2505237 - 185 Protein_2435 DUF3596 domain-containing protein -
A4C47_RS12860 2505272..2505523 - 252 WP_000005552.1 excisionase family protein -
A4C47_RS12865 2505596..2508067 - 2472 WP_070080217.1 exonuclease -
A4C47_RS12870 2508160..2508351 - 192 WP_001090200.1 DUF1482 family protein -
A4C47_RS12875 2508348..2508536 - 189 WP_000449192.1 cell division inhibition protein DicB -
A4C47_RS12880 2509105..2509323 - 219 WP_001171930.1 hypothetical protein -
A4C47_RS12885 2509395..2509694 - 300 WP_001240334.1 hypothetical protein -
A4C47_RS12895 2510047..2510325 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
A4C47_RS12900 2510327..2510518 - 192 WP_001302048.1 hypothetical protein Antitoxin
A4C47_RS29095 2510539..2510910 - 372 WP_001169686.1 hypothetical protein -
A4C47_RS12910 2511008..2511310 + 303 WP_000172738.1 transcriptional regulator -
A4C47_RS12915 2511307..2511732 + 426 WP_000693943.1 Rha family transcriptional regulator -
A4C47_RS12920 2511755..2512717 + 963 WP_000095669.1 helix-turn-helix domain-containing protein -
A4C47_RS12925 2512724..2513464 + 741 WP_000788938.1 ATP-binding protein -
A4C47_RS12935 2513490..2514259 + 770 Protein_2449 DUF1627 domain-containing protein -
A4C47_RS12940 2514275..2514670 + 396 WP_001118159.1 DUF977 family protein -
A4C47_RS12945 2514727..2515311 + 585 WP_000206793.1 DUF551 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - nleG7' 2495167..2608462 113295


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T66745 WP_001303511.1 NZ_CP017446:c2510325-2510047 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T66745 NZ_CP017446:c2510325-2510047 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT66745 WP_001302048.1 NZ_CP017446:c2510518-2510327 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT66745 NZ_CP017446:c2510518-2510327 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References