Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2511330..2511801 Replicon chromosome
Accession NZ_CP017438
Organism Escherichia coli O157:H7 strain 2159

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag A4C45_RS12910 Protein ID WP_001303511.1
Coordinates 2511330..2511608 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag A4C45_RS12915 Protein ID WP_001302048.1
Coordinates 2511610..2511801 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A4C45_RS30975 2506336..2506520 - 185 Protein_2436 DUF3596 domain-containing protein -
A4C45_RS12875 2506555..2506806 - 252 WP_000005552.1 excisionase family protein -
A4C45_RS12880 2506879..2509350 - 2472 WP_070080217.1 exonuclease -
A4C45_RS12885 2509443..2509634 - 192 WP_001090200.1 DUF1482 family protein -
A4C45_RS12890 2509631..2509819 - 189 WP_000449192.1 cell division inhibition protein DicB -
A4C45_RS12895 2510388..2510606 - 219 WP_001171930.1 hypothetical protein -
A4C45_RS12900 2510678..2510977 - 300 WP_001240334.1 hypothetical protein -
A4C45_RS12910 2511330..2511608 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
A4C45_RS12915 2511610..2511801 - 192 WP_001302048.1 hypothetical protein Antitoxin
A4C45_RS29125 2511822..2512193 - 372 WP_001169686.1 hypothetical protein -
A4C45_RS12925 2512291..2512593 + 303 WP_000172738.1 transcriptional regulator -
A4C45_RS12930 2512590..2513015 + 426 WP_000693943.1 Rha family transcriptional regulator -
A4C45_RS12935 2513038..2514000 + 963 WP_000095669.1 helix-turn-helix domain-containing protein -
A4C45_RS12940 2514007..2514747 + 741 WP_000788938.1 ATP-binding protein -
A4C45_RS12950 2514773..2515542 + 770 Protein_2450 DUF1627 domain-containing protein -
A4C45_RS12955 2515558..2515953 + 396 WP_001118159.1 DUF977 family protein -
A4C45_RS12960 2516010..2516594 + 585 WP_000206793.1 DUF551 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - nleG7' 2506365..2605476 99111


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T66611 WP_001303511.1 NZ_CP017438:c2511608-2511330 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T66611 NZ_CP017438:c2511608-2511330 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT66611 WP_001302048.1 NZ_CP017438:c2511801-2511610 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT66611 NZ_CP017438:c2511801-2511610 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References