121490

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2471761..2472232 Replicon chromosome
Accession NZ_CP038324
Organism Escherichia coli O157:H7 strain NE 1169-1

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag E4U74_RS12225 Protein ID WP_001303511.1
Coordinates 2471761..2472039 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag E4U74_RS12230 Protein ID WP_001302048.1
Coordinates 2472041..2472232 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4U74_RS12190 2466986..2467237 - 252 WP_000005552.1 excisionase family protein -
E4U74_RS12195 2467310..2469781 - 2472 WP_000048458.1 exonuclease -
E4U74_RS12200 2469874..2470065 - 192 WP_001090200.1 DUF1482 family protein -
E4U74_RS12205 2470062..2470250 - 189 WP_000449192.1 cell division inhibition protein DicB -
E4U74_RS12210 2470651..2470815 + 165 WP_001331716.1 hypothetical protein -
E4U74_RS12215 2470819..2471037 - 219 WP_001171930.1 hypothetical protein -
E4U74_RS12220 2471109..2471408 - 300 WP_001240334.1 hypothetical protein -
E4U74_RS12225 2471761..2472039 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
E4U74_RS12230 2472041..2472232 - 192 WP_001302048.1 hypothetical protein Antitoxin
E4U74_RS12235 2472253..2472624 - 372 WP_001169686.1 hypothetical protein -
E4U74_RS12240 2472722..2473024 + 303 WP_000172738.1 transcriptional regulator -
E4U74_RS12245 2473021..2473446 + 426 WP_000693943.1 Rha family transcriptional regulator -
E4U74_RS12250 2473469..2474431 + 963 WP_000095669.1 helix-turn-helix domain-containing protein -
E4U74_RS12255 2474438..2475178 + 741 WP_000788938.1 ATP-binding protein -
E4U74_RS12260 2475204..2475973 + 770 Protein_2399 DUF1627 domain-containing protein -
E4U74_RS12265 2475989..2476384 + 396 WP_001118159.1 DUF977 family protein -
E4U74_RS12270 2476441..2477025 + 585 WP_000206793.1 DUF551 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - nleG7' 2456881..2561031 104150


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T121490 WP_001303511.1 NZ_CP038324:c2472039-2471761 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T121490 NZ_CP038324:c2472039-2471761 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT121490 WP_001302048.1 NZ_CP038324:c2472232-2472041 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT121490 NZ_CP038324:c2472232-2472041 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References