88860

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 4683..5230 Replicon plasmid tig00000311
Accession NZ_CP024854
Organism Escherichia coli strain AR_0006

Toxin (Protein)


Gene name parE Uniprot ID E9LLH3
Locus tag AM333_RS27330 Protein ID WP_000969996.1
Coordinates 4949..5230 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID K4JYR0
Locus tag AM333_RS27325 Protein ID WP_000079928.1
Coordinates 4683..4952 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM333_RS27290 555..1754 - 1200 WP_000948429.1 IS91 family transposase -
AM333_RS27295 1764..1952 - 189 WP_000957857.1 hypothetical protein -
AM333_RS27310 2743..3774 + 1032 WP_000907874.1 replication initiation protein -
AM333_RS28435 4470..4628 + 159 WP_000841008.1 hypothetical protein -
AM333_RS27325 4683..4952 + 270 WP_000079928.1 hypothetical protein Antitoxin
AM333_RS27330 4949..5230 + 282 WP_000969996.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AM333_RS27335 5270..6118 + 849 WP_001057996.1 3'-5' exonuclease -
AM333_RS27340 6476..7675 - 1200 WP_000948429.1 IS91 family transposase -
AM333_RS27345 7685..7873 - 189 WP_000957857.1 hypothetical protein -
AM333_RS27350 8270..9415 + 1146 WP_000976514.1 class C beta-lactamase CMY-2 -
AM333_RS27355 9509..10042 + 534 WP_001221666.1 lipocalin family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Conjugative plasmid blaCMY-2 - 1..96413 96413
- inside IScluster/Tn blaCMY-2 - 555..9415 8860


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10893.57 Da        Isoelectric Point: 8.4615

>T88860 WP_000969996.1 NZ_CP024854:4949-5230 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T88860 NZ_CP024854:4949-5230 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCTGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10175.50 Da        Isoelectric Point: 7.3210

>AT88860 WP_000079928.1 NZ_CP024854:4683-4952 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT88860 NZ_CP024854:4683-4952 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCACGGCGATGTGCTTCCCCATGACATGGTCGAATCCTCTGCTGCAGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E9LLH3

Antitoxin

Source ID Structure
AlphaFold DB A0A5W2J4D0

References