30466

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 46461..46725 Replicon plasmid pJIE512b
Accession NC_025198
Organism Escherichia coli

Toxin (Protein)


Gene name pndA Uniprot ID E6BRV3
Locus tag H4J97_RS00295 Protein ID WP_001303307.1
Coordinates 46573..46725 (+) Length 51 a.a.

Antitoxin (RNA)


Gene name pndB
Locus tag -
Coordinates 46461..46523 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4J97_RS00280 (D616_p157067) 41700..43991 - 2292 WP_072107381.1 F-type conjugative transfer protein TrbC -
H4J97_RS00285 (D616_p157068) 43984..45054 - 1071 WP_000151576.1 IncI1-type conjugal transfer protein TrbB -
H4J97_RS00290 (D616_p157069) 45073..46281 - 1209 WP_000121273.1 IncI1-type conjugal transfer protein TrbA -
- 46461..46523 - 63 - - Antitoxin
H4J97_RS00295 (D616_p157071) 46573..46725 + 153 WP_001303307.1 Hok/Gef family protein Toxin
H4J97_RS00300 (D616_p157072) 46797..47048 - 252 WP_001291967.1 hypothetical protein -
H4J97_RS00565 47547..47642 + 96 WP_001303310.1 DinQ-like type I toxin DqlB -
H4J97_RS00305 (D616_p157074) 47707..48051 - 345 WP_181394993.1 hypothetical protein -
H4J97_RS00310 (D616_p157075) 48226..48435 + 210 WP_000062602.1 HEAT repeat domain-containing protein -
H4J97_RS00315 (D616_p157076) 48507..49169 - 663 WP_032073207.1 plasmid IncI1-type surface exclusion protein ExcA -
H4J97_RS00320 (D616_p157077) 49234..51396 - 2163 WP_000698351.1 DotA/TraY family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Conjugative plasmid blaCMY-2 - 1..92339 92339


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(7-46)


Sequences

Toxin        


Download         Length: 51 a.a.        Molecular weight: 5817.21 Da        Isoelectric Point: 8.7948

>T30466 WP_001303307.1 NC_025198:46573-46725 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Download         Length: 153 bp

>T30466 NC_025198:46573-46725 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 63 bp

>AT30466 NC_025198:c46523-46461 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A634Q5L1

Antitoxin

Download structure file

References