Detailed information of TA system TA140168

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 75302..75780
• Replicon: plasmid pAR-0430-1
• Accession: NZ_CP044137
• Organism: Escherichia coli O157 strain AR-0430

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: F4V42_RS00490
• Protein ID: WP_072743077.1
• Coordinates: 75302..75514 (-)
• Length: 71 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: K4JYR0
• Locus tag: F4V42_RS00495
• Protein ID: WP_000079928.1
• Coordinates: 75511..75780 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V42_RS00465	71259..71576	+	318	WP_000118520.1	quaternary ammonium compound efflux SMR transporter SugE	-
F4V42_RS00470	71573..72106	-	534	WP_001221666.1	lipocalin family protein	-
F4V42_RS00475	72200..73345	-	1146	WP_000976514.1	class C beta-lactamase CMY-2	-
F4V42_RS00480	73741..73929	+	189	WP_000957857.1	hypothetical protein	-
F4V42_RS00485	73939..75138	+	1200	WP_000948429.1	IS91 family transposase	-
F4V42_RS00490	75302..75514	-	213	WP_072743077.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F4V42_RS00495	75511..75780	-	270	WP_000079928.1	hypothetical protein	Antitoxin
F4V42_RS29910	75835..75993	-	159	WP_000841008.1	hypothetical protein	-
F4V42_RS00510	76689..77720	-	1032	WP_000907874.1	replication initiation protein	-
F4V42_RS00525	78693..78956	+	264	WP_001324596.1	ash family protein	-
F4V42_RS00530	78874..79161	+	288	WP_000074849.1	conjugal transfer protein TraA	-
F4V42_RS00540	79603..80136	+	534	WP_001303319.1	transcription termination factor NusG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_025198 Escherichia coli Conjugative plasmid TA010119, TA030466 1..92339 0.004	inside	Conjugative plasmid	blaCMY-2	-	1..88229	88229
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_025198 Escherichia coli IS/Tn TA010119 6856..9795 0	flank	IS/Tn	blaCMY-2	-	72200..75138	2938

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-66)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 8118.32 Da
• Isoelectric Point: 7.3170

>T140168 WP_072743077.1 NZ_CP044137:c75514-75302 [Escherichia coli O157]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVHV

Nucleotide

• Length: 213 bp

>T140168 NZ_CP059000:7885-8445 [Mycobacterium avium subsp. hominissuis]
GTGAGCGATGCGCTCGACGAAGATCTGCTCCAGCGCATCGACGCGCGTGGAACCACCCCCTGGTCGGCGACATGCTTCCG
ATACACCGGTGCACATCGTGACCCTCTGTCGGGCGAGGGAGCTCGCCGATTCGGCGGCAGGTGGAATCCTCCCCTGCTGT
TCTCCGCGATCTACCTTTCCGACTCGGCCCAGGCGTGCATGGTCGAAGTAGGACGAGCAGCCCAGGCAGCGTCGACGACC
GCCGAGAAGCTGCTCGAAGCGAGCTACCGACTGCACACCGTCGAAGCGACCGACCTGGCTGTCCTGGATCTCACCACCCC
CGAGGCTCGCGACACGGTAGGGCTTGAAGATGACGACATCCACGGCGAGGACTGGTCGGCCTGCCAAGCAGTGGGCCACG
CCGCATGGTTTCTGCACATGCAAGGCATACTGGTGCCCGCAGCCGGCGGAGTCGGCCTCGTCATCACCGCCTACGAACAA
CGCACCCGACCCGGCCAGCTCCAGGTCAGCCACAGCGAGGATCTGACCCCCGCCCGCTACCAAGAACTACGGCACAAATG
A

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10175.50 Da
• Isoelectric Point: 7.3210

>AT140168 WP_000079928.1 NZ_CP044137:c75780-75511 [Escherichia coli O157]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT140168 NZ_CP059000:7547-7888 [Mycobacterium avium subsp. hominissuis]
ATGGGAGTTAACGCGCTCGCCTCGACGGTGTCCAGTGCGATCGAGCGCCTGGGATTGACCTACGAGGAGGTCGGCGGCAT
CGTCGATGCGTCTGCGCGCTCGGTGGCGCGCTGGACCTCAGGGCAGGTCGTCCCGCAACGCCTCAACAAGCAACGACTGA
TCGAGCTGGCCTACGTTGCCGACGCGCTGGCCGAAGTCCTTCCCCGTGACCAGGCCAACGTGTGGATGTTCTCGCCGAAT
CGACTACTGCAGCACGCCAAACCTGCCGACCTGGTACGCGACGGTGAGTATCAGCGCGTCTTGGCGCTGATTGACGCGAT
GGCCGAAGGGATCTTCGTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A5W2J4D0