140164

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 35280..35544 Replicon plasmid pAR-0430-1
Accession NZ_CP044137
Organism Escherichia coli O157 strain AR-0430

Toxin (Protein)


Gene name pndA Uniprot ID U9Y6M3
Locus tag F4V42_RS00195 Protein ID WP_001331364.1
Coordinates 35280..35432 (-) Length 51 a.a.

Antitoxin (RNA)


Gene name pndB
Locus tag -
Coordinates 35482..35544 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F4V42_RS00175 30559..32721 + 2163 WP_000698351.1 DotA/TraY family protein -
F4V42_RS00180 32786..33447 + 662 Protein_31 plasmid IncI1-type surface exclusion protein ExcA -
F4V42_RS00185 33519..33728 - 210 WP_000062603.1 HEAT repeat domain-containing protein -
F4V42_RS29895 34120..34296 + 177 WP_001054904.1 hypothetical protein -
F4V42_RS00190 34361..34657 - 297 WP_001275298.1 hypothetical protein -
F4V42_RS29900 35068..35208 + 141 WP_000880644.1 hypothetical protein -
F4V42_RS00195 35280..35432 - 153 WP_001331364.1 Hok/Gef family protein Toxin
- 35482..35544 + 63 NuclAT_0 - Antitoxin
- 35482..35544 + 63 NuclAT_0 - Antitoxin
- 35482..35544 + 63 NuclAT_0 - Antitoxin
- 35482..35544 + 63 NuclAT_0 - Antitoxin
F4V42_RS00200 35724..36932 + 1209 WP_000121273.1 IncI1-type conjugal transfer protein TrbA -
F4V42_RS00205 36951..38021 + 1071 WP_000151583.1 IncI1-type conjugal transfer protein TrbB -
F4V42_RS00210 38014..40305 + 2292 WP_001289276.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Conjugative plasmid blaCMY-2 - 1..88229 88229


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(7-46)


Sequences

Toxin        


Download         Length: 51 a.a.        Molecular weight: 5775.13 Da        Isoelectric Point: 8.7948

>T140164 WP_001331364.1 NZ_CP044137:c35432-35280 [Escherichia coli O157]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Download         Length: 153 bp

>T140164 NZ_CP058994:c856751-856458 [Pasteurella multocida]
ATGAGTAAGAAAAAAATAAAATTAAAACTGTTTGATATTGCTGAACATCTAGACAGTGAAGAAATGATTGCGGCATATTT
AAGTGAAGTTTTAGAAACATGTGATACTAATGAATTTATTTCCGCTTTAGCTGATGTTGTAAGAGCAAAAGGAATGACGG
AGTTAGCAGCAAAAACAGGGTTAAGAAGAGAAAGCCTCTATAAAACATTATCATACGGTAGCAAGCCACGTTTTGATACG
ATAATGAAAATTACGCATTCATTAGGTATTAAACTTGTCCCAACGCACATCTAA

Antitoxin


Download         Length: 63 bp

>AT140164 NZ_CP044137:35482-35544 [Escherichia coli O157]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5W9M382

Antitoxin

Download structure file

References